Fast-migrating species upon agarose-gel electrophoresis was yielded at 20mM MgCl2 condition.
Correctly folded structures were yielded at 20mM of MgCl2, because they were more compact than misfolded versions.
(The structures at high concentration (30 and 40mM) of MgCl2 tend to aggregate because of positive charge.)
--->> Optimum concentration of MgCl2: 20mM
To decide optimum incubate temparature
Fast-migrating species upon agarose-gel electrophoresis was yielded at 47.7 or 51.6 degree C. The
band at 47.7 degree C is more clear than that of at 51.6 degree C.
The band of 47.7 degree C is fast migrated and strong.
--->> Optimum temparature : 47.7 degree C
To decide optimum length of incubate time
The structure assembled for 4h becomes most compact. The band for 4h is fast migrated and strong.