Biomod/2013/StJohns/introduction

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Near-Term Goals)
(Near-Term Goals)
Line 20: Line 20:
====Near-Term Goals====
====Near-Term Goals====
-
* [[Biomod/2013/StJohns/approaches#Virus-Binding Claw|The basic claw design itself:]]
+
* [[Biomod/2013/StJohns/approaches#Claw|The basic claw design itself:]]
**Synthesis of the claw with (‘sticky’) and without (‘blunt’) single-stranded binding elements.
**Synthesis of the claw with (‘sticky’) and without (‘blunt’) single-stranded binding elements.
**Synthesis of the claw with and without fluorescent tags for FRET analysis.  
**Synthesis of the claw with and without fluorescent tags for FRET analysis.  
Line 35: Line 35:
**Demonstrate that we can isolate anti-Flag FAB fragments reliably and effectively.
**Demonstrate that we can isolate anti-Flag FAB fragments reliably and effectively.
**Attach small model ketones to DNA strands as a preliminary to attaching FAB fragments.
**Attach small model ketones to DNA strands as a preliminary to attaching FAB fragments.
-
* [[Biomod/2013/StJohns/approaches#Triangles|Methods of controlling the vertex angles of DO structures for precise hinge angles:]]
+
* [[Biomod/2013/StJohns/approaches#Triangle Tiles|Methods of controlling the vertex angles of DO structures for precise hinge angles:]]
**Synthesize small DO triangle elements with precise geometries, and verify these geometries on AFM.
**Synthesize small DO triangle elements with precise geometries, and verify these geometries on AFM.

Revision as of 22:56, 26 October 2013

For optimal viewing experience, please enlarge this window to at least 1024 pixels.

Contents

Motivation

There are many ways to detect viruses, but they rely on detecting small pieces of the virus rather than the whole structure. If we could reliably detect the entire virus rather than just a part, this could be applied to the rapid diagnosis of viral diseases as well as rapid detection of infectious agents in the environment.

Project Overview

The goal of this project is to design and characterize a DNA origami[1] (DO) structure that undergoes significant conformational changes when bound to objects ranging in size from 10-100 nm.

Since binding-specific conformational change can be transduced into a signal, this should enable the design of nanometre-scale sensors for viruses.

In our proof-of-principle approach, the origami structure is a three-pronged DO ‘claw’[2] with sticky-ended DNA strands complementary to the surface of a modified[3] bacteriophage MS2 capsid substrate.

To characterize the conformational changes and binding interactions of this system, we will use four primary methods:

Near-Term Goals

Youtube Video

Personal tools