Biomod/2013/StJohns/introduction: Difference between revisions
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*[http://en.wikipedia.org/wiki/Dynamic_light_scattering Dynamic Light Scattering] | *[http://en.wikipedia.org/wiki/Dynamic_light_scattering Dynamic Light Scattering] | ||
====Youtube | ====Youtube Video==== | ||
<html><center><iframe class="youtube-player" style="text-align:center" type="text/html" width="420" height="315" src="http://www.youtube.com/embed/pdfXxF9UCWk" frameborder="0" allowfullscreen></iframe></center></html> | <html><center><iframe class="youtube-player" style="text-align:center" type="text/html" width="420" height="315" src="http://www.youtube.com/embed/pdfXxF9UCWk" frameborder="0" allowfullscreen></iframe></center></html> | ||
Revision as of 11:43, 26 October 2013
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<html><a href="http://lukemanlab.org"><img src="http://openwetware.org/images/thumb/f/f9/Lukemanlab-toehold-conga-nanny-header.jpg/180px-Lukemanlab-toehold-conga-nanny-header.jpg"></a></html>
Motivation
There are a number of techniques for detecting antigenic elements in solution, but we are not aware of any methods of rapidly detecting an entire structure presenting those elements in a particular arrangement as opposed to merely detecting each antigenic region separately. Such a technique would, among other applications, allow more reliable detection of pathogens in vitro or in the environment, potentially allowing rapid diagnosis of viral infection and easy testing for the presence of infectious agents. This project comprises an initial foray into developing such a technique.
Abstract
The goal of this project is to design and characterize a DNA origami[1] (DO) structure that undergoes significant conformational changes when bound to objects ranging in size from 10-100 nm.
Since binding-specific conformational change can be transduced into a signal, this should enable the design of nanometre-scale sensors for viruses.
In our proof-of-principle approach, the origami structure is a three-pronged DO ‘claw’[2] with sticky-ended DNA strands complementary to the surface of a modified[3] bacteriophage MS2 capsid substrate.
In parallel, we will address several other aspects of the longer-term project:
- We will investigate methods of selecting the most avid claws from a ‘library’ of designs with different binding-site geometries.
- We will investigate immunoglobulins as potential binding elements for use in future claw designs.
- We will investigate methods of controlling the vertex angles of DO structures using computer-aided design.
To characterize the conformational changes and binding interactions of this system, we will use four primary methods:
- Gel Electrophoresis
- Atomic Force Microscopy
- Förster Resonance Energy Transfer (FRET) analysis
- Dynamic Light Scattering
Youtube Video
<html><center><iframe class="youtube-player" style="text-align:center" type="text/html" width="420" height="315" src="http://www.youtube.com/embed/pdfXxF9UCWk" frameborder="0" allowfullscreen></iframe></center></html>
