Destroy the liposomeFollowing mathematical formula reveals proves that smaller liposomes are more stable. From this calculation result, we think it is possible to destroy liposomes if we provide with energy by trigger. ・Hypothesis We assume that a vesicle of large radius existing underwater changes to the plural vesicles of small radius (fig 1). The free energy of each states F1: large radius, and F2: small radius, probably have the following relationship (fig 2). In our case, the vesicle is basically small state because a small radius is more stable.The decisive factor is, however, that there is the energy gap “δ” that must exceed to change the size of vesicle. In our calculation we have not had the possibility to calculate δ, but to calculate the free energy in each radius. With this values of energy and radius the energy gap "δ" can be deducted.
|Fig.1 Assumed state of vesicle in the system||Fig.2 Assumed relation of each free energy|
・Calculation result Free energy “F” of the vesicle is given as follows*.(*Reference)
In this time,
(pf)bulk is a partition function of the single discrete molecule underwater, and (pf)vesicle is a partition function of the vesicle. Therefore, free energy “F”,
In this calculation, we assume that N, T, and V are constant,
Because of α= 0 at the moment of N0 – N = 0
This time we approximate,
N/n=X means the number of the vesicles in whole of this system. Therefore we calculate free energy by using this,
Here, a vesicle which radius is 100μm changes to each 10 and 100 vesicles, calculate each free energy,
From these results, we get the follow (fig 3). Fig.3 The relation of each free energy by calculation
From fig 3, free energy is smaller when the radius of vesicle is small. ・Discussion When all molecules participate in the formation of the vesicle, the free energy becomes a one-tenth time and the number of vesicle increase 10 times. Smaller radius vesicles are more stable.Therefore, it is possible to destroy vesicles using trigger.
・Reference Yukio Suezaki "Physics of lipid film"