Biomod/2013/Sendai

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             <a href="http://openwetware.org/wiki/Biomod/2013/Sendai"><h1 style="color:white;" ><b>Biomod<span>2013<br>&emsp; Team</span>Sendai</b></h1></a>  
             <a href="http://openwetware.org/wiki/Biomod/2013/Sendai"><h1 style="color:white;" ><b>Biomod<span>2013<br>&emsp; Team</span>Sendai</b></h1></a>  
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<br><div class="title">Lipo-HANABI</div>
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          <p>私達のプロジェクトはトリガー入力による連鎖的分子放出システムの構築を目指している。 既存のDDSでは薬剤放出のタイミングの制御が困難な事、一度の入力に対して一定の出力しか得られない事が課題である。</br>
 
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この問題を克服する為、私達はある条件でトリガーDNAを放出するシステムと、その放出を起点に連鎖的にリポソームが割れる出力増幅システムの2つを構築する。</br>
 
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1つ目のシステムでは、トリガーを内在したアルギン酸ゲル膜を作り、温度の上昇に伴い自発的なゲルの融解とトリガーの放出が行われる。2つ目のシステムは、リポソーム表面にトリガーをハイブリし物理的な負荷をかけ、リポソームを破壊する。破壊されるリポソームは新たなトリガーを含んでおり、放出されたトリガーは連鎖的に周囲のリポソームを破壊する。</br>
 
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本システムの実現により入力のタイミングが制御可能かつ一度の入力で大量の薬剤を出力できるDDSの創出等への応用が期待できる。</br>
 
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Our project aims to construct a chain-reactive molecule-releasing system in a spontaneous manner. Delivering drugs at appropriate places and controlling the quantity of released drug is very important to develop effective DDS. Our system makes these possible.<br>
 
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The system consists of two sub-systems: “egg-type initiator” and “chain-reactive burst”. <br>
 
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The “egg-type initiator” releases trigger DNAs under a certain condition like warming up to body temperature. The trigger DNAs hybridize to aptamer DNAs on liposomes, and the hybridization causes liposomes collapse. The collapsed liposomes release drugs and new trigger stored inside. These processes are the “chain-reactive burst”, and achieve sequential and exponential release of drugs. <br>
 
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To realize the egg-type initiator, trigger DNAs and chelate compounds are encapsulated in temperature-sensitive liposomes, and the liposomes are encapsulated in alginate hydrogel. Increasing temperature disrupts the liposomes, and then chelate compounds inside the liposomes melt the gels. Collapse of liposomes is realized by rapid deformation of liposomes mediated by hybridization between trigger and aptamer DNAs. <br>
 
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Our system will propose a new place and quantity controlling system of drug release by liposome-based drug delivery systems.<br>
 
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We are aiming at constructing a chain-reactive molecule-releasing system in this project. <br>
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In living organisms, signal transduction is widely used in many situations. <br>
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Here we construct a signal transduction-like system through an approach of DNA nano-engineering. <br>
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In our project, trigger DNAs and payload molecules are encapsulated in a liposome. The trigger DNA is specially designed to collapse the liposome when some initiation stimulus is applied. Once one of the liposomes collapsed, the trigger DNAs are released from the liposome and that break other liposomes in chain-reactive fashion. This mechanism of “chain-reactive burst” enables us to release a large amount of payload molecules all at once, driven by a faint trigger signal. <br>
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We believe this system offers a fundamental technique of signal transduction/amplification of various kinds of chemical or physical signals, applicable to Drug Delivery System, molecular computers and so on.<br>
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Revision as of 23:06, 26 October 2013

Biomod2013 Sendai ver2.0

Biomod2013
  Team
Sendai



Lipo-HANABI

Abstract

We are aiming at constructing a chain-reactive molecule-releasing system in this project.
In living organisms, signal transduction is widely used in many situations.
Here we construct a signal transduction-like system through an approach of DNA nano-engineering.
In our project, trigger DNAs and payload molecules are encapsulated in a liposome. The trigger DNA is specially designed to collapse the liposome when some initiation stimulus is applied. Once one of the liposomes collapsed, the trigger DNAs are released from the liposome and that break other liposomes in chain-reactive fashion. This mechanism of “chain-reactive burst” enables us to release a large amount of payload molecules all at once, driven by a faint trigger signal.
We believe this system offers a fundamental technique of signal transduction/amplification of various kinds of chemical or physical signals, applicable to Drug Delivery System, molecular computers and so on.

Movie

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