Biomod/2013/OSU/protocol/folding reaction

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<h1>Folding Reaction</h1>
<h1>Folding Reaction</h1>
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<td>p01</td>
<td>p01</td>
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<td>p085</td>
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<td>A1</td>
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<td>H12</td>
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<td>CoreP085</td>
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<td>96</td>
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<td>1.04</td>
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<td>core 1</td>
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<td>p02</td>
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<td>p085</td>
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<td>A1</td>
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<td>H12</td>
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<td>CoreP086</td>
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<td>41</td>
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<td>2.44</td>
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<td>core 2</td>
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<td>p03</td>
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<td>p086</td>
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<td>Neighbors</td>
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<td>7.69</td>
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<td>Neighbors</td>
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<td>p04</td>
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<td>p086</td>
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<td>Polymers</td>
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<td>13</td>
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<td>7.69</td>
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<td>Replaced by polymers</td>
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Revision as of 22:19, 6 October 2013


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Folding Reaction

A folding reaction is a mixture containing scaffold DNA, staple DNA, water, a buffer mastermix, and magnesium ions. The folding involves subjecting the sample mixture to thermal denaturation followed by renaturing. We will usually have the folding reactions occur in PCR strip tubes of either 50-100 μL aliquots.

Pre-stocks

A folding reaction is a mixture containing scaffold DNA, staple DNA, water, a buffer mastermix, and magnesium ions. The folding involves subjecting the sample mixture to thermal denaturation followed by renaturing. We will usually have the folding reactions occur in PCR strip tubes of either 50-100 μL aliquots.

Index Plate From Well To Well Name No. of Oligos Conc. (um) Element
p01 p085 A1 H12 CoreP085 96 1.04 core 1
p02 p085 A1 H12 CoreP086 41 2.44 core 2
p03 p086 E1 F1 Neighbors 13 7.69 Neighbors
p04 p086 G1 H1 Polymers 13 7.69 Replaced by polymers


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