Biomod/2013/OSU/protocol/drugloading

From OpenWetWare

< Biomod | 2013 | OSU | protocol
Revision as of 10:29, 26 October 2013 by Mike W. Zoller (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search


<< Back to Team Pages

Loading Efficiency

  1. Setup a 2 mM Daunorubicin Solution (Given Concentration of Solution)
  2. Add Origami shapes at a concentration of 5.0 nM
  3. Incubate for 40 hours
  4. Centrifuge at 20.0 xG for 50 minutes @ 20 deg C
  5. Isolate Orange-red precipitate from solution - PEG (Keep solution—gives concentration of daunorubicin not absorbed by origami/used in loading efficiency calculation)
  6. Read Absorption at 488 nm with a microplate reader (FRET Setting)


Retrieved from "http://openwetware.org/wiki/Biomod/2013/OSU/protocol/drugloading"
Views
Personal tools