Biomod/2013/OSU/protocol/custom tem grids
Revision as of 19:58, 24 October 2013 by
Mike W. Zoller
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Preparing Custom Grids for TEM
Coat grids with a thin collodion plastic film
minimum 20 cm diameter bowl/basin
TEM copper grids 200 or 400
vellum paper (Pergament-Papier)
Completely fill basin with ddH2O right to the rim.
Use razor blade to shorten a 200er pipet tip to create a 3mm diameter aperture.
Pipet out 50 μL collodion solution.
Eject the collodion from approx 10 cm onto the calm water surface. CAUTION: collodion is toxic!
Ensure proper ventilation and hold breath while handling the collodion!
A collodion film will spread across the water surface and harden. Wait for 5 min.
Use forcepts to position copper grids onto the collodion film. Put shiny (specular-reflecting) side of the grids facing the collodion. Arrange in a neat hexagonal or square pattern.
Position vellum paper on top of girds. Let soak with water.
Put collodion film around the circumference of the vellum paper by using the razor blade.
Carefully pick up the vellum paper at one corner/edge and pull out of the water.
Turn the vellum paper with the grids facing up, put into petri dish, partially cover and let dry overnight in a clean dust free area.
Please direct all inquiries to our group email at
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Mike W. Zoller
Paul M Gruenbacher
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