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In this section we want to give a short summary of the project. As we already were able to do some first steps into future experiments we will also give some details how the project will be continued. | |||
=Goals reached= | |||
The first question to answer if we want to qualify our developement is if the goals set at the beginning were reached. Due to the inhomogenity of the material and the foreseeable obstacles that come along with that we limited the goal of our project to a feasible extent. Namely we wanted to develope a proper functionalization of the nanodiamonds surface fulfilling three conditions: | |||
#It has to be biocompatible to allow a future in vivo use.<br /> | |||
#The functionalized nanodiamonds must offer surface groups which can be linked to specific docking sites of the DNA-origami.<br /> | |||
#The functionalization should cause the nanodiamonds to be well dispersed, as single nanodiaomds should be attached to DNA-origami.<br /> | |||
All this conditions are fulfilled by the developed coating. The included materials in the process are fully biocompatible and intended for the use at physiological conditions. In addition to that the coated diamonds dispersed well and thus could be bound specifically to our designed 6HB. This could be verified by TEM measurements. So we can report specific binding of functionalized nanodiamonds to DNA-Origami for the very first time.<br /> | |||
Our experiment was aimed to proof the principle but we are already made some progress in improving the yield of our method for use in further experiments. Additionally we were already able to roughly determine the excitation and absortion spectra of the used fluorescence diamonds. This opens the ground for the next steps heading to realize the structure of Schietinger et al. (2009) in a self arranging structure. | |||
=Next steps= | |||
[[File:goldND.jpg|thumb|400px|right|Model of the self assembling nano diamond gold hybrid structure]] | |||
Since the initial motivation of the project was based on realizing diamond nanoparticles hybrid structures in a self assembling way we are already planning the next steps. For this we will work together with the group of Prof. Högele which has the know-how and necessary equipment on disposal to carry out both detailed optical characterisation and single photon measurements at the nano-scale.<br /> | |||
The long term objective is to synthesize a 6HB structure with nanodiamonds surrounded by nano gold particles on both sides. A visualization of that can be seen on the pictures to the right. <br /> | |||
With this in mind we first tried to make some fluorescence pictures of the assembled nanodiamond 6HB structures. But the spectrum of the emission turned out to be a problem, since it is not in the measurement range of the available fluorescence microscope. Despite this due to the cooperation with Prof. Högele we are very confident to find a solution for that during the next weeks. After that we will be able to do the first single photon measurements which constitute the basis for the interpretation of the following experiments with gold diamond arrangements. Regarding this we have already done some first experiments adding gold particles to our structures but it turned out that the goldparticles clustered with our nanodiamonds and thus inhibited the specific attachment to the 6HB. But once we will have jumped over this last obstacle we will have the set up needed for a demonstration of the expected improvement of both excitation and emission rate of the NVC radiation as already demonstrated in the publication of Schietinger at al. of 2009. If we can realize that it will be the first time to demonstrate that on a self arranged structure. | |||
Revision as of 15:01, 22 October 2013
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In this section we want to give a short summary of the project. As we already were able to do some first steps into future experiments we will also give some details how the project will be continued.
Goals reached
The first question to answer if we want to qualify our developement is if the goals set at the beginning were reached. Due to the inhomogenity of the material and the foreseeable obstacles that come along with that we limited the goal of our project to a feasible extent. Namely we wanted to develope a proper functionalization of the nanodiamonds surface fulfilling three conditions:
- It has to be biocompatible to allow a future in vivo use.
- The functionalized nanodiamonds must offer surface groups which can be linked to specific docking sites of the DNA-origami.
- The functionalization should cause the nanodiamonds to be well dispersed, as single nanodiaomds should be attached to DNA-origami.
All this conditions are fulfilled by the developed coating. The included materials in the process are fully biocompatible and intended for the use at physiological conditions. In addition to that the coated diamonds dispersed well and thus could be bound specifically to our designed 6HB. This could be verified by TEM measurements. So we can report specific binding of functionalized nanodiamonds to DNA-Origami for the very first time.
Our experiment was aimed to proof the principle but we are already made some progress in improving the yield of our method for use in further experiments. Additionally we were already able to roughly determine the excitation and absortion spectra of the used fluorescence diamonds. This opens the ground for the next steps heading to realize the structure of Schietinger et al. (2009) in a self arranging structure.
Next steps
Since the initial motivation of the project was based on realizing diamond nanoparticles hybrid structures in a self assembling way we are already planning the next steps. For this we will work together with the group of Prof. Högele which has the know-how and necessary equipment on disposal to carry out both detailed optical characterisation and single photon measurements at the nano-scale.
The long term objective is to synthesize a 6HB structure with nanodiamonds surrounded by nano gold particles on both sides. A visualization of that can be seen on the pictures to the right.
With this in mind we first tried to make some fluorescence pictures of the assembled nanodiamond 6HB structures. But the spectrum of the emission turned out to be a problem, since it is not in the measurement range of the available fluorescence microscope. Despite this due to the cooperation with Prof. Högele we are very confident to find a solution for that during the next weeks. After that we will be able to do the first single photon measurements which constitute the basis for the interpretation of the following experiments with gold diamond arrangements. Regarding this we have already done some first experiments adding gold particles to our structures but it turned out that the goldparticles clustered with our nanodiamonds and thus inhibited the specific attachment to the 6HB. But once we will have jumped over this last obstacle we will have the set up needed for a demonstration of the expected improvement of both excitation and emission rate of the NVC radiation as already demonstrated in the publication of Schietinger at al. of 2009. If we can realize that it will be the first time to demonstrate that on a self arranged structure.
