Biomod/2013/LMU/equipment: Difference between revisions
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==AFM== | ==AFM== | ||
An [http://en.wikipedia.org/wiki/Atomic_force_microscopy AFM] (Atomic Force Microscope) is a type of scanning probe microscope which can have a sub-nanometer resolution. We used it to survey our origami-structures and its modifications. | An [http://en.wikipedia.org/wiki/Atomic_force_microscopy AFM] (Atomic Force Microscope) is a type of scanning probe microscope which can have a sub-nanometer resolution. We used it to survey our origami-structures and its modifications.<br /> | ||
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[[Image:afm2.JPG|350px|center]] | [[Image:afm2.JPG|350px|center]]<br /> | ||
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==Nanodrop (Spectrometer)== | ==Nanodrop (Spectrometer)== | ||
Revision as of 15:48, 25 October 2013
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Lab Equipment
TEM
The transmission electron microscopy (TEM) is used for taking images of the nanodiamonds and/or the resulting DNA-origami structures. As the TEM offers nanometer resolution the nanodiamonds can be easily resolved. However the contrast of DNA structures is too low. In order to make them visible, one has to stain the samples with uranyl acetate.
AFM
An AFM (Atomic Force Microscope) is a type of scanning probe microscope which can have a sub-nanometer resolution. We used it to survey our origami-structures and its modifications.
Nanodrop (Spectrometer)
Nanodrop is a spectrophotometer. One can measure the absorption spectra of any solution in the visible and near UV-range. Many materials have a characterisitc absorption spectrum. We used this fact to check which chemicals are left after purification of a sample. Furthermore one can determine the nucleid acid concentration of a sample.
Centrifuge
In order to purify and/or wash our samples we had to centrifuge them several times for a few minutes (Link to protocols). Typically we used it We used it frequently in order to remove the chemicals which were left over free material after functionlizing the nanodiamonds.
Gel Electropeheresis
Gel electrophoresis is a method to seperate DNA-structures by their size and charge. Depending on the size of the DNA-structures we had to use two different types of gel. As polyacrylamide gel has typically a comparatively small pore size it is ideally suited to seperate small DNA fragments or molecules. In contrast to that, we had to use agarose gel to analyze our origami structures.
Software
caDNAano
caDNAano is 3D design tool for DNA-origami. We used it for designing our origami structures.
