Biomod/2013/Komaba/Experiment
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- This content is under construction. We have already ordered DNAs of some parts and they will arrive soon. On arrival, we will conduct experiments.
Lab Notes
We conducted a lot experiment at Fuji Laboratory, Komaba Research Campus.
September 11th Cylinder and Ring(first ver.)
Staple mix
(μL)
| Cylinder | Ring | ||
|---|---|---|---|
| Staple | 2.5μL(each) | 50μM | |
| TE buffer | 52.5μL | ||
| Total | 500μL |
Stock of the staples are 50μM.
There were 179 strands for the Cylinder and 80 strands for the Ring.
Cylinder(10 - equivalent)
| Mg[math]\displaystyle{ 2+ }[/math] | TAE10x | M13 | Staple mix | mQ | Total | |
|---|---|---|---|---|---|---|
| Consentraion | 12.5mM | 2.5nM | 25nM | |||
| Amount | 1.25μL | 10μL | 6.25μL | 10μL | 72.5μL | 100μL |
| Stock | 1M | 40nM | 250nM |
