Biomod/2013/Komaba/Experiment: Difference between revisions
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<tr> | <tr> | ||
<th> | <th> | ||
</th><th>Concentration</th><th>TAE10x | </th><th> Concentration</th><th>TAE10x | ||
</th><th>Amount | </th><th> Amount | ||
</th><th>Stock | </th><th> Stock | ||
</th></tr> | </th></tr> | ||
<tr> | <tr> | ||
<td>Mg<math>2+</math> | <td> Mg<math>2+</math> | ||
</td><td>12.5mM | </td><td> 12.5mM | ||
</td><td>1.25μL | </td><td> 1.25μL | ||
</td><td>1M | </td><td> 1M | ||
</td></tr> | </td></tr> | ||
<tr> | <tr> | ||
<td>TAE10x | <td> TAE10x | ||
</td><td> | </td><td> | ||
</td><td>10μL | </td><td> 10μL | ||
</td></tr> | </td></tr> | ||
<tr> | <tr> | ||
<td>M13 | <td> M13 | ||
</td><td>2.5nM | </td><td> 2.5nM | ||
</td><td>6.25μL | </td><td> 6.25μL | ||
</td><td>40nM | </td><td> 40nM | ||
</td></tr> | </td></tr> | ||
<tr> | <tr> | ||
<td>Staple Mix | <td> Staple Mix | ||
</td><td>25nM | </td><td> 25nM | ||
</td><td>10μL | </td><td> 10μL | ||
</td><td>250nM | </td><td> 250nM | ||
</td></tr> | </td></tr> | ||
<tr> | <tr> | ||
<td>mQ | <td> mQ | ||
</td><td> | </td><td> | ||
</td><td>72.5μL | </td><td> 72.5μL | ||
</td><td> | </td><td> | ||
</td></tr> | </td></tr> | ||
<tr> | <tr> | ||
<td>Total | <td> Total | ||
</td><td> | </td><td> | ||
</td><td>100μL | </td><td> 100μL | ||
</td><td> | </td><td> | ||
</td></tr></table> | </td></tr></table> | ||
<p> <br> | <p> <br> | ||
</p> | </p> | ||
Revision as of 11:16, 16 October 2013
Home,Project,Design,Experiment,Supplementation,Team,Sponsors
- This content is under construction. We have already ordered DNAs of some parts and they will arrive soon. On arrival, we will conduct experiments.
Lab Notes
We conducted a lot experiment at Fuji Laboratory, Komaba Research Campus.
September 11th Cylinder and Ring(first ver.)
Staple mix (Cylinder)
| Amount | Stock | ||
|---|---|---|---|
| Staple | 2.5μL(each) | 50μM | |
| TE buffer | 52.5μL | ||
| Total | 500μL |
Stock of the staples are 50μM.
There were 179 strands for the Cylinder.
Cylinder(10 - equivalent)
| Concentration | TAE10x | Amount | Stock | |
|---|---|---|---|---|
| Mg[math]\displaystyle{ 2+ }[/math] | 12.5mM | 1.25μL | 1M | |
| TAE10x | 10μL | |||
| M13 | 2.5nM | 6.25μL | 40nM | |
| Staple Mix | 25nM | 10μL | 250nM | |
| mQ | 72.5μL | |||
| Total | 100μL |
