Biomod/2013/Komaba/Experiment: Difference between revisions
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<h2> <span class="mw-headline">September 11th Cylinder and Ring(first ver.)</span></h2> | <h2> <span class="mw-headline">September 11th Cylinder and Ring(first ver.)</span></h2> | ||
===Staple mix=== | ===Staple mix (Cylinder)=== | ||
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<p>Stock of the staples are 50μM.<br>There were 179 strands for the Cylinder | <p>Stock of the staples are 50μM.<br>There were 179 strands for the Cylinder. | ||
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Revision as of 11:06, 16 October 2013
Home,Project,Design,Experiment,Supplementation,Team,Sponsors
- This content is under construction. We have already ordered DNAs of some parts and they will arrive soon. On arrival, we will conduct experiments.
Lab Notes
We conducted a lot experiment at Fuji Laboratory, Komaba Research Campus.
September 11th Cylinder and Ring(first ver.)
Staple mix (Cylinder)
| Amount | Stock | ||
|---|---|---|---|
| Staple | 2.5μL(each) | 50μM | |
| TE buffer | 52.5μL | ||
| Total | 500μL |
Stock of the staples are 50μM.
There were 179 strands for the Cylinder.
Cylinder(10 - equivalent)
| Mg[math]\displaystyle{ 2+ }[/math] | TAE10x | M13 | Staple mix | mQ | Total | |
|---|---|---|---|---|---|---|
| Consentraion | 12.5mM | 2.5nM | 25nM | |||
| Amount | 1.25μL | 10μL | 6.25μL | 10μL | 72.5μL | 100μL |
| Stock | 1M | 40nM | 250nM |
