Biomod/2013/Komaba/Experiment: Difference between revisions
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| Line 13: | Line 13: | ||
<tr> | <tr> | ||
<th> | <th> | ||
</th><th> | </th><th>Cylinder | ||
</th><th> | </th><th>Ring | ||
</th></tr> | </th></tr> | ||
<tr> | <tr> | ||
<td> | <td>Staple | ||
</td><td>2. | </td><td>2.5μL(each) | ||
</td><td> | </td><td>50μM | ||
</td></tr> | </td></tr> | ||
<tr> | <tr> | ||
<td> | <td>TE buffer | ||
</td><td> | </td><td>52.5μL | ||
</td><td> | </td><td> | ||
</td><td> | |||
</td></tr> | |||
<tr> | |||
<td>Total | |||
</td><td>500μL | |||
</td><td> | |||
</td><td> | |||
</td></tr></table> | </td></tr></table> | ||
<p>Stock of the staples are 50μM.<br>There were 179 strands for the Cylinder and 80 strands for the Ring. | <p>Stock of the staples are 50μM.<br>There were 179 strands for the Cylinder and 80 strands for the Ring. | ||
Revision as of 11:02, 16 October 2013
Home,Project,Design,Experiment,Supplementation,Team,Sponsors
- This content is under construction. We have already ordered DNAs of some parts and they will arrive soon. On arrival, we will conduct experiments.
Lab Notes
We conducted a lot experiment at Fuji Laboratory, Komaba Research Campus.
September 11th Cylinder and Ring(first ver.)
Staple mix
(μL)
| Cylinder | Ring | ||
|---|---|---|---|
| Staple | 2.5μL(each) | 50μM | |
| TE buffer | 52.5μL | ||
| Total | 500μL |
Stock of the staples are 50μM.
There were 179 strands for the Cylinder and 80 strands for the Ring.
Cylinder(10 - equivalent)
| Mg[math]\displaystyle{ 2+ }[/math] | TAE10x | M13 | Staple mix | mQ | Total | |
|---|---|---|---|---|---|---|
| Consentraion | 12.5mM | 2.5nM | 25nM | |||
| Amount | 1.25μL | 10μL | 6.25μL | 10μL | 72.5μL | 100μL |
| Stock | 1M | 40nM | 250nM |
