Biomod/2013/Komaba/Experiment: Difference between revisions
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<tr> | <tr> | ||
<th> | <th> | ||
</th><th><math> | </th><th>Mg<math>2+</math> | ||
</th><th>TAE10x | |||
</th><th>M13 | |||
</th><th>Staple mix | |||
</th><th>mQ | |||
</th><th>Total | |||
</th></tr> | |||
<tr> | |||
<td>Consentraion | |||
</td><td>12.5mM | |||
</td><td> | |||
</td><td>2.5nM | |||
</td><td>25nM | |||
</td><td> | |||
</td><td> | |||
</td></tr> | |||
<tr> | |||
<td>Amount | |||
</td><td>1.25μL | |||
</td><td>10μL | |||
</td><td>6.25μL | |||
</td><td>10μL | |||
</td><td>72.5μL | |||
</td><td>100μL | |||
</td></tr> | |||
<tr> | |||
<td>Stock | |||
</td><td>1M | |||
</td><td> | |||
</td><td>40nM | |||
</td><td>250nM | |||
</td><td> | |||
</td><td> | |||
</td></tr></table> | |||
<p> <br> | |||
</p> | |||
Revision as of 10:31, 16 October 2013
Home,Project,Design,Experiment,Supplementation,Team,Sponsors
- This content is under construction. We have already ordered DNAs of some parts and they will arrive soon. On arrival, we will conduct experiments.
Lab Notes
We conducted a lot experiment at Fuji Laboratory, Komaba Research Campus.
September 11th Cylinder and Ring(first ver.)
Staple mix
(μL)
| Staple | TE buffer | Total Amount | |
|---|---|---|---|
| Cylinder | 2.5(each) | 52.5 | 500 |
| Ring | 5.0(each) | 100 | 500 |
Stock of the staples are 50μM.
There were 179 strands for the Cylinder and 80 strands for the Ring.
Cylinder(10 - equivalent)
| Mg[math]\displaystyle{ 2+ }[/math] | TAE10x | M13 | Staple mix | mQ | Total | |
|---|---|---|---|---|---|---|
| Consentraion | 12.5mM | 2.5nM | 25nM | |||
| Amount | 1.25μL | 10μL | 6.25μL | 10μL | 72.5μL | 100μL |
| Stock | 1M | 40nM | 250nM |
