Biomod/2013/Komaba/Design: Difference between revisions
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== DNA Spider == | == DNA Spider == | ||
: | : Our DNA screw rotates by using DNA spiders produced by Lund, et al.. DNA Spider consists of a core and three legs. These legs move on the footing by cutting strands with DNAzyme. Since legs orbit the cylinder, they rotate the DNA Ring. | ||
In this paragraph, how to create DNA spiders is described. This method is the same as the way Lund, et al. did. First, Streptavidin(STV) and capture leg [5’ - GCC GAG AAC CTG ACG CAA GT/iSp18//iSp18//3Bio/ - 3] are connected in a solution. To create one-to-one product (“STV-(C)1” ), ion exchange is used. Second, deoxyribozyme legs [5’ - /5BioTEG//iSp18//iSp18//TCT CTT CTC CGA GCC GGT CGA AAT AGT GAA AA – 3’ ] are attached to the STV-(C) i.e. NICK3.4A+1. Third, Cy3 MONO NHS ester dissolved in DMSO is added in the solution containing NICK3.4A+1. | |||
== Rotary Ring == | == Rotary Ring == | ||
Revision as of 01:55, 30 August 2013
Home,Project,Design,Experiment,Supplementation,Team,Sponsors
Cylinder
- The cylinder is put in the center of the motor as an axis supporting the rotation. The material DNA is formed into a cylindrical shape using DNA Origami technology. It was designed using cadnano software, which "simplifies and enhances the process of designing three-dimensional DNA origami nanostructures".(Figure 1)
- In order to bind footing DNAs on its surface spirally, 10mer long DNA strands which we call footings of footings come up from the cylinder's surface. After the cylinder with the footing of footings are formed, one of the three legs of DNA spiders is connected to a specific strand at the start point on the cylinder's surface. Then the footings get connected to the footing of footings. The footing tracks, each of which consists of three lanes of the footings, enable DNA Spiders to orbit the cylinder. In fact, there are two tracks of DNA footings and two spiders can walk on each track. In addition, in order to make it easy to observe the direction of spiders' walk, a small sub-cylinder is attached at the end of the footing tracks.
- The diameter of the cylinder is 25.4 nm and height is 43.5 nm. This is calculated on the assumptions that the cylinder can be observed with an Atomic Force Microscope and that the interval between the two tracks are wide enough for spiders not to jump to next footing lane.
(Figure 2)
- [Figure 1:cadnanoのデータ][Figure 2:シリンダーの絵]
DNA Spider
- Our DNA screw rotates by using DNA spiders produced by Lund, et al.. DNA Spider consists of a core and three legs. These legs move on the footing by cutting strands with DNAzyme. Since legs orbit the cylinder, they rotate the DNA Ring.
In this paragraph, how to create DNA spiders is described. This method is the same as the way Lund, et al. did. First, Streptavidin(STV) and capture leg [5’ - GCC GAG AAC CTG ACG CAA GT/iSp18//iSp18//3Bio/ - 3] are connected in a solution. To create one-to-one product (“STV-(C)1” ), ion exchange is used. Second, deoxyribozyme legs [5’ - /5BioTEG//iSp18//iSp18//TCT CTT CTC CGA GCC GGT CGA AAT AGT GAA AA – 3’ ] are attached to the STV-(C) i.e. NICK3.4A+1. Third, Cy3 MONO NHS ester dissolved in DMSO is added in the solution containing NICK3.4A+1.
Rotary Ring
- It is the DNA ring part that actually rotates. It has shape like the side surface of a disk, and is also composed with DNA Origami technology. 10mer staple strand for connecting DNA Spider is attached on the inner side of the Ring. The diameter of the ring is 50.9 nm, and so it can hold the cylinder and spiders in the inside of the ring. The thickness of ring is 10.9 nm in consideration of AFM visibility.
Cylinder Motor (Combination of above parts)
- The motor is realized by assembling above three parts: Cylinder, footings, DNA Spider, and Rotary Ring.
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<div id="nfooter"> <h1>DNA Screw - Komaba Team</h1> </div> <div id="globalWrapper"><div id="column-content"><div id="content"><div id="bodyContent"></html>
