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All linker variations showed a decrease in luminescence level, but they all exhibited significant decrease in activity when exposed to high concentration of calcium. Unfortunately, the protein switch does not respond to increased levels of M13, | All linker variations showed a decrease in luminescence level, but they all exhibited significant decrease in activity when exposed to high concentration of calcium. Unfortunately, the protein switch does not respond to increased levels of M13, but this is a promising start for continued modification to the output domain. | ||
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Revision as of 17:13, 20 October 2013
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HARVARD
BIODESIGN
BIOMOD 2013
-
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard"><li>HOME</li></a></html>
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard/introduction"><li>INTRODUCTION</li></a></html>
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard/design"><li>DESIGN</li></a></html>
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard/methods"><li>METHODS</li></a></html>
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard/protocols"><li>PROTOCOLS</li></a></html>
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard/team"><li>TEAM</li></a></html>
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard/lab"><li>LAB NOTEBOOK</li></a></html>
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard/results"><li>RESULTS</li></a></html>
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard/references"><li>REFERENCES</li></a></html>
Results
Input
Input results
Output
Cysteine Mutations
Linker Variations
Another possibility we explored was varying the linker length and rigidity. The original GLucCam structure has amino acid strands GGGGT and GGGS as the first and second linker sequence. We explored the following linker variations:
Short-rigid: Short-flexible:
All linker variations showed a decrease in luminescence level, but they all exhibited significant decrease in activity when exposed to high concentration of calcium. Unfortunately, the protein switch does not respond to increased levels of M13, but this is a promising start for continued modification to the output domain.
