Biomod/2013/Harvard/lab: Difference between revisions
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| Line 17: | Line 17: | ||
'''I. Bacterial Solution: LB Agar Plates''' | '''I. Bacterial Solution: LB Agar Plates''' | ||
:purpose: to make agar plates for 1L LB-agar | :purpose: to make agar plates for 1L LB-agar | ||
# | # Mix stock | ||
## 960mL DI water (860 mL if using glucose optional) | ## 960mL DI water (860 mL if using glucose optional) | ||
## 10g of Tryptone | ## 10g of Tryptone | ||
| Line 24: | Line 24: | ||
## 15g agar | ## 15g agar | ||
# | # Autoclave | ||
## | ## liquid (not dry) 30 min. sterilization/20 min drying | ||
## cool to ~50°C in water bath | ## cool to ~50°C in water bath | ||
Revision as of 07:30, 18 June 2013
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HARVARD
BIODESIGN
BIOMOD 2013
-
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard"><li>HOME</li></a></html>
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard/introduction"><li>INTRODUCTION</li></a></html>
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard/design"><li>DESIGN</li></a></html>
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard/methods"><li>METHODS</li></a></html>
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard/protocols"><li>PROTOCOLS</li></a></html>
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard/team"><li>TEAM</li></a></html>
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard/lab"><li>LAB NOTEBOOK</li></a></html>
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard/results"><li>RESULTS</li></a></html>
<html><a href="http://openwetware.org/wiki/Biomod/2013/Harvard/references"><li>REFERENCES</li></a></html>
Laboratory Notebook
Week 1
6/10/13
General BioMOD Cloning
- purpose: clone for DH5α pDIMC8 BlaCaM His and DH5α pDIMC8 DsbA BlaCaM
- 5 mL LB
- 100µL 20% glucose
- Cm (34 mg/mL working stock), 7.35 µL of this, final concentration 50 µg/mL
- Add cells
- Inoculate overnight
6/11/13
I. Bacterial Solution: LB Agar Plates
- purpose: to make agar plates for 1L LB-agar
- Mix stock
- 960mL DI water (860 mL if using glucose optional)
- 10g of Tryptone
- 10g of NaCl
- 5g yeast extract
- 15g agar
- Autoclave
- liquid (not dry) 30 min. sterilization/20 min drying
- cool to ~50°C in water bath
Week 2
Bla
