Biomod/2013/Fukuoka

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*In this study, we tried to fabricate the device in which an inorganic porous substrate is combined with DNA origami. We synthesize the “DNA weathercock” which has a shaft, a blade, and a fluorescence molecule made of DNA origami. We then attach the weathercock onto the inorganic porous substrate (anodizing alumina) with regularly arranged perpendicular pores, so that the weathercock can freely rotate. We incorporate fluorescence quencher molecules on to a part of the substrate, so that the device emit fluorescence only when the liquid on the substrate flows along a specific direction.
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*In this study, we tried to fabricate the device in which an inorganic porous substrate is combined with DNA origami. We synthesize the DNA-origami-based “DNA weathercock” which has a shaft, a blade, and a fluorescence molecule We then attach the weathercock onto the inorganic porous substrate (anodizing alumina) with regularly arranged perpendicular pores, so that the weathercock can freely rotate. We incorporate fluorescence quencher molecules on to a part of the substrate, so that the device emits fluorescence only when the liquid on the substrate flows along a specific direction.
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=Methods=
=Methods=
*Create a DNA origami of fluorescent molecules likened to weathercock, attach it to the hole of the anodizing alumina. Attach the fluorescent molecule to weathercock to detect fine flow as light. Also include the quencher molecule in a part of the anodizing alumina. How to install the quencher molecule is something to block the pores of the part in one direction by the DNA origami obtained by modifying a fluorescent molecule on a substrate, and incorporate the quencher molecule to it. Quencher molecule is mounted on hole of the substrate not blocked by the DNA origami. Weathercock of DNA with fluorescent molecules to free rotation on the substrate incorporated the quencher molecule. If there is a flow to the left as a, the flash fires because it is in a part without a quencher molecule is the tip of the DNA origami. If there is a flow to the right as b, does not flash because it is in a part quencher molecule is the tip of the DNA origami. This way, detect the flow.  
*Create a DNA origami of fluorescent molecules likened to weathercock, attach it to the hole of the anodizing alumina. Attach the fluorescent molecule to weathercock to detect fine flow as light. Also include the quencher molecule in a part of the anodizing alumina. How to install the quencher molecule is something to block the pores of the part in one direction by the DNA origami obtained by modifying a fluorescent molecule on a substrate, and incorporate the quencher molecule to it. Quencher molecule is mounted on hole of the substrate not blocked by the DNA origami. Weathercock of DNA with fluorescent molecules to free rotation on the substrate incorporated the quencher molecule. If there is a flow to the left as a, the flash fires because it is in a part without a quencher molecule is the tip of the DNA origami. If there is a flow to the right as b, does not flash because it is in a part quencher molecule is the tip of the DNA origami. This way, detect the flow.  

Revision as of 10:56, 31 August 2013

Contents

Abstract

  • Recent years, the technology of DNA origami is attracting keen interest for the synthesis of various nanostructures. If we integrate movable structures in a DNA origami, various applications such as molecular detection devices, molecular motor devices, and drug delivery systems are expected. However, only a few examples of such a movable system have been reported so far. Other problem is that the synthesis of DNA origami in larger amount is not so easy. Hence, it is unrealistic to make the macroscopic-scale device which output or input macroscopic signals such as a mechanical one, only by using DNA origamis. Therefore, in this study, we tried to fabricate the device in which an inorganic porous substrate is combined with DNA origami. We synthesize the DNA-origami-based “DNA weathercock” which has a shaft, a blade, and a fluorescence molecule. We then attach the weathercock onto an inorganic porous substrate (anodizing alumina) with regularly arranged perpendicular pores, so that the weathercock can freely rotate. We incorporate fluorescence quencher molecules on to a part of the substrate, so that the device emits fluorescence only when the liquid on the substrate flows along a specific direction.

Introduction

Background

  • Recently, DNA origami are attracting attentions. DNA-origami is the programmable nanostructure which is synthesized by weaving a very long single strand DNA with a large number of short single strand DNAs, just like the weft of the textile. While the technique originally allowed us to form flat nanostructures, we can recently fabricate complicated three-dimensional nanostructures with bent parts like a bird cage.

Problems

  • However, only a few examples of a DNA-origami system with a movable part in themselves have been reported so far. It is also a problem that the synthesis of DNA origami in a large amount is not so easy, the creation of the macroscopic-scale device only with DNA origami which output or input the macroscopic signals such as a mechanical one.

Approaches

  • In this study, we tried to fabricate the device in which an inorganic porous substrate is combined with DNA origami. We synthesize the DNA-origami-based “DNA weathercock” which has a shaft, a blade, and a fluorescence molecule We then attach the weathercock onto the inorganic porous substrate (anodizing alumina) with regularly arranged perpendicular pores, so that the weathercock can freely rotate. We incorporate fluorescence quencher molecules on to a part of the substrate, so that the device emits fluorescence only when the liquid on the substrate flows along a specific direction.

Methods

  • Create a DNA origami of fluorescent molecules likened to weathercock, attach it to the hole of the anodizing alumina. Attach the fluorescent molecule to weathercock to detect fine flow as light. Also include the quencher molecule in a part of the anodizing alumina. How to install the quencher molecule is something to block the pores of the part in one direction by the DNA origami obtained by modifying a fluorescent molecule on a substrate, and incorporate the quencher molecule to it. Quencher molecule is mounted on hole of the substrate not blocked by the DNA origami. Weathercock of DNA with fluorescent molecules to free rotation on the substrate incorporated the quencher molecule. If there is a flow to the left as a, the flash fires because it is in a part without a quencher molecule is the tip of the DNA origami. If there is a flow to the right as b, does not flash because it is in a part quencher molecule is the tip of the DNA origami. This way, detect the flow.

Results and Disscution

  • We compared to design this shape. An enlarged view portion surrounded by a blue figure on the left is the figure on the right. Of irregular shape similar to DNA origami design was observed. The figure below is a cross-sectional view of a portion of the green line in the right figure. The size of the design is Length 28 nm, Side 20 nm and Height 17 nm. Cross-sectional view of the observed image is Length about 30 nm, Side about 21 nm, Height 2.3 nm. Diameter is almost the same but height is not match. It is seems to be because the base pairs of the DNA with each other are aggregated.

Sponsor

JPK Instruments AG (JPKインスツルメンツ)
株式会社 レベルファイブ
株式会社 生体分子計測研究所 (RIBM)
株式会社 和泉テック
右に流れがある場合
右に流れがある場合
左に流れがある場合
左に流れがある場合
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