Biomod/2013/Fukuoka

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[[Image:drop江口.gif|400px|right]]
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=Abstract=
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*Recent years, the technology of DNA origami is attracting keen interest for the synthesis of various nanostructures.    If we integrate movable structures in a DNA origami, various applications such as molecular detection devices, molecular motor devices, and drug delivery systems are expected. However, only a few examples of such a movable system have been reported so far. Other problem is that the synthesis of DNA origami in larger amount is not so easy. Hence, it is unrealistic to make the  macroscopic-scale device which output or input macroscopic signals such as a mechanical one, only by using DNA origamis. Therefore, in this study, we tried to fabricate the device in which an inorganic porous substrate is combined with DNA origami. We synthesize the DNA-origami-based “DNA weathercock” which has a shaft, a blade, and a fluorescence molecule. We then attach the weathercock onto an inorganic porous substrate (anodizing alumina) with regularly arranged perpendicular pores, so that the weathercock can freely rotate. We incorporate fluorescence quencher molecules on to a part of the substrate, so that the device emits fluorescence only when the liquid on the substrate flows along a specific direction.
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=Introduction=
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<table cellspacing="7" cellpadding="10" width="920" >
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==Background==
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<tr align="center">
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*Recently, DNA origami are attracting attentions. DNA-origami is the programmable nanostructure which is synthesized by weaving a very long single strand DNA with a large number of short single strand DNAs, just like the weft of the textile. While the technique originally allowed us to form flat nanostructures, we can recently fabricate complicated three-dimensional nanostructures with bent parts like a bird cage.
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<td width="180" bgcolor="#002233">[[Biomod/2013/Fukuoka|<font face="cursive,fantasy,Arial" color="white">Top</font>]] </td>
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<td width="180" bgcolor="#002233">[[Introduction|<font face="cursive,fantasy,Arial" color="white">Introduction</font>]] </td>
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<td width="180" bgcolor="#002233">[[Our Approach and Goals|<font face="cursive,fantasy,Arial" color="white">Approach and Goals</font>]] </td>
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<td width="180" bgcolor="#002233">[[Method|<font face="cursive,fantasy,Arial" color="white">Method</font>]] </td>
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<td width="180" bgcolor="#002233">[[Results and Discussion|<font face="cursive,fantasy,Arial" color="white">Results and Discussion</font>]] </td>
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<td width="180" bgcolor="#002233">[[Member|<font face="cursive,fantasy,Arial" color="white">Member</font>]] </td>
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<td width="180" bgcolor="#002233">[[Sponsor|<font face="cursive,fantasy" color="white">Sponsor</font>]] </td>
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==Problems==
 
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*However, only a few examples of a DNA-origami system with a movable part in themselves have been reported so far. It is also a problem that the synthesis of DNA origami in a large amount is not so easy, the creation of the macroscopic-scale device only with DNA origami which output or input the macroscopic signals such as a mechanical one.
 
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=Approaches=
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</table>
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*In this study, we tried to fabricate the device in which an inorganic porous substrate is combined with DNA origami. We synthesize the DNA-origami-based “DNA weathercock” which has a shaft, a blade, and a fluorescence molecule. We then attach the weathercock onto the inorganic porous substrate (anodizing alumina) with regularly arranged perpendicular pores, so that the weathercock can freely rotate. We incorporate fluorescence quencher molecules on to a part of the substrate, so that the device emits fluorescence only when the liquid on the substrate flows along a specific direction.
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=Results and Disscution=
 
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*Fig.1 shows the AFM image of the synthesized DNA-weathercock. We observe many small objects, while we also find some aggregated large objects. On the enlarged AFM image (Fig.2), the object with the shape similar to the DNA origami designed with caDNAno (Fig.3) was observed. The Fig.4 is the cross-sectional view for the green line on Fig.2. While the size of the designed object has the length of 28 nm, width of 20 nm and the height 17 nm, the observed image is mostly in the same size.
 
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<gallery>
 
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Image:拡大図左.png|fig.1 AFM image of the synthesized DNA-weathercock
 
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Image:拡大図中.png|fig.2 the enlarged AFM image
 
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Image:拡大図右.png|fig.3 designed with caDNAno
 
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Image:断面図.jpg|fig.4 cross-sectional view for the green line on Fig.2
 
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</gallery>
 
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=Member=
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=Abstract=
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'''Students'''
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*Recent years, the technology of DNA origami is attracting keen interest for the synthesis of various nanostructures. However, there are only few example of DNA origami with a rotating part, although the rotational motion is important in natural and industrial machineries such as  flagellum movement of Eugelena, turbines, and motors. Another problem is that the creation of a macroscopic-scale device only with DNA origami, which works with the macroscopic output or input signals, is difficult because the synthesis of DNA-origami in large amount is too expensive. Here, we present a simple rotating nano-device, DNA-weathercock, which detects the flow of surrounding water, by combining inorganic nanoporous substrate material with nanoscale weathercock made of DNA origami. The DNA weathercock consist of a blade and shaft parts are attached with a fluorescence molecule. We mount the DNA weathercock by sticking the shaft part into the nanopore of the size-tunable anodizing alumina, so that the DNA weathercock can freely rotate. When we give a flow, the DNA weathercock turns to the direction of the flow. We observe the behavior of the fluorescence molecule attached to the weathercock head with a confocal laser microscope or even with our naked-eyes through a polarizers.
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<br><!--<youtube>jD0lJCXIu6U</youtube>-->
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*Maika Kuroki
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<html><iframe width="640" height="360" src="//www.youtube.com/embed/jD0lJCXIu6U?feature=player_detailpage" frameborder="0" allowfullscreen></iframe></html>
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*Shinya Anraku
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*Ryo Iwashita
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*Eiji Horita
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'''Adviser'''
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*Naoya Yamaguchi
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'''Professor'''
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*Hajime Mita
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*Nobuyoshi Miyamoto
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=Sponsor=
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[[Image:JPK_logo2013.jpg|none|200px|JPK Instruments AG (JPKインスツルメンツ)]]
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*http://japan.jpk.com/index.2.ja.html
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[[Image:level5-logo2013.jpg|none|200px|株式会社 レベルファイブ]]
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*http://www.level-five.jp/
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[[Image:RIBM_logo2013.jpg|none|200px| 株式会社 生体分子計測研究所 (RIBM)]]
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*http://www.ribm.co.jp/
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[[Image:和泉テック_logo2013.jpg|none|200px| 株式会社 和泉テック]]
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*http://www.izumi-tech.com/index.html
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Current revision


Top Introduction Approach and Goals Method Results and Discussion Member Sponsor


Abstract

  • Recent years, the technology of DNA origami is attracting keen interest for the synthesis of various nanostructures. However, there are only few example of DNA origami with a rotating part, although the rotational motion is important in natural and industrial machineries such as flagellum movement of Eugelena, turbines, and motors. Another problem is that the creation of a macroscopic-scale device only with DNA origami, which works with the macroscopic output or input signals, is difficult because the synthesis of DNA-origami in large amount is too expensive. Here, we present a simple rotating nano-device, DNA-weathercock, which detects the flow of surrounding water, by combining inorganic nanoporous substrate material with nanoscale weathercock made of DNA origami. The DNA weathercock consist of a blade and shaft parts are attached with a fluorescence molecule. We mount the DNA weathercock by sticking the shaft part into the nanopore of the size-tunable anodizing alumina, so that the DNA weathercock can freely rotate. When we give a flow, the DNA weathercock turns to the direction of the flow. We observe the behavior of the fluorescence molecule attached to the weathercock head with a confocal laser microscope or even with our naked-eyes through a polarizers.