Biomod/2013/Aarhus/Supplementary/Supplementary Data

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Supplementary data

Origami design

Table S1. Details of the different modules of the plate.
Table S2. Details of the dome structure.
Table S3.Details of the different modules.

sisiRNA

Yields of RP-HPLC purifications of the modified strands

Table S4: Yields of RP-HPLC purifications of the 12 modified strands.

Estimated yields of the annealing reactions

Table S5. Estimated yields of the annealing reactions.

Luciferase knockdown with all singly modified duplexes

Fig. S1. Cell viability corrected luciferase activities for all singly modified duplexes, transfected with Lipofectamine. Click on the image and choose 'high resolution' for details.

Table S6. Best fit IC50 values of all knockdown experiments using singly modified duplexes using Lipofectamine.The IC50 value for W179-PEG1K was calculated with a low R2 value and is not conclusive.

Fig. S2. Luciferase and MTT measurements of all knockdown experiments with singly modified duplexes using Lipofectamine.

System in action

Radioactive labeling of W376

Fig. S3. 1) 25 bp DNA ladder, 2) 6 µL labeled W376, 3) 1 pmol W376, 4) 1.5 pmol W376, 5) pmol W376, 6) 2.5 pmol W376, 7) pmol W376.

The band containing the labeled sample appears most similar to that in lane 4 which means that the 6 µL that were run on the gel contained 1.5 pmol W376, corresponding to a concentration of 0.25 µM in the purified sample.

Cholesterol

1H NMR, 13C NMR and IR of compound 4.

<html><A name="NMR4H"/></html>

1H NMR for compound 4

<html><A name="NMR4C"/></html>

13C NMR for compound 4
Zoom of 13C NMR for compound 4

<html><A name="IR4"/></html>

IR for compound 4

1H NMR, 13C NMR and IR of compound 5.

<html><A name="NMR5H"/></html>

1H NMR for compound 5

<html><A name="NMR5C"/></html>

13C NMR for compound 5
Zoom of 13C NMR for compound 5

<html><A name="IR5"/></html>

IR for compound 5

1H NMR and 13C NMR of compound 6.

<html><A name="NMR6H"/></html>

1H NMR for compound 6

<html><A name="NMR6C"/></html>

13C NMR for compound 6
Zoom of 13C NMR for compound 6

Photosensitizer

1H NMR and 13C NMR of compound 7.

<html><A name="NMR7H"/></html>

1H NMR of compound 7

<html><A name="NMR7C"/></html>

13C NMR of compound 7

1H NMR and 13C NMR of compound 8.

<html><A name="NMR8H"/></html>

1H NMR of compound 8

<html><A name="NMR8C"/></html>

13C NMR of compound 8

1H NMR, 13C NMR and UV of compound 9.

<html><A name="NMR9H"/></html>

1H NMR of compound 9

<html><A name="NMR9C"/></html>

13C NMR of compound 9

<html><A name="9UV"/></html> UV of compound 9


1H NMR, 13C NMR and UV of compound 10.

<html><A name="NMR10H"/></html>

1H NMR for compound 10

<html><A name="NMR10C"/></html>

13C NMR for compound 10

<html><A name="10UV"/></html> UV for compound 10


1H NMR and 13C NMR of compound 11.

<html><A name="NMR11H"/></html>

1H NMR for compound 11

<html><A name="NMR11C"/></html>

13C NMR for compound 11

1H NMR and 13C NMR of compound 12.

<html><A name="NMR12H"/></html>

1H NMR for compound 12

<html><A name="NMR12C"/></html>

13C NMR for compound 12

5-propargylamino-ddUTP

1H NMR, 13C NMR and IR of compound 15.

<html><A name="NMR15H"/></html>

1H NMR of compound 15
zoom of 1H NMR of compound 15

<html><A name="NMR15C"/></html>

13C NMR of compound 15

<html><A name="IR15"/></html>

IR of compound 15

1H NMR, 13C NMR and IR of compound 16.

<html><A name="NMR16H"/></html>

1H NMR of compound 16
zoom of 1H NMR of compound 16

<html><A name="NMR16C"/></html>

13C NMR of compound 16

<html><A name="IR16"/></html>

IR of compound 16

1H NMR, 13C NMR and IR of compound 17.

<html><A name="NMR17H"/></html>

1H NMR of compound 17
zoom of 1H NMR for compound 17

<html><A name="NMR17C"/></html>

13C NMR of compound 17

<html><A name="IR17"/></html>

IR for compound 17

1H NMR, 13C NMR and IR of compound 18.

<html><A name="NMR18H"/></html>

1H NMR of compound 18
zoom of 1H NMR for compound 18

<html><A name="NMR18C"/></html>

13C NMR of compound 18

<html><A name="IR18"/></html>

IR for compound 18

1H NMR and 19F NMR of compound 19.

<html><A name="NMR19H"/></html>

1H NMR of compound 19

<html><A name="NMR19F"/></html>

19F NMR of compound 19

1H NMR and 19F NMR of compound 21.

<html><A name="NMR21H"/></html>

1H NMR of compound 21

<html><A name="NMR21F"/></html>

19F NMR of compound 21

Peptide lock

Spectra

(S)-2-((tert-butoxycarbonyl)amino)-5-methoxy-5-oxopentanoic acid

1H NMR

1H NMR of (S)-2-((tert-butoxycarbonyl)amino)-5-methoxy-5-oxopentanoic acid

13C NMR

13C NMR of (S)-2-((tert-butoxycarbonyl)amino)-5-methoxy-5-oxopentanoic acid




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</style> </head> <body> <div id="indexing"> <div id="sitemap"> <p id="sitemapTitle">SITEMAP | BIOMOD 2013 NANO CREATORS | Aarhus University</p> <div id="footer-contents"> <div class="footer-section"> <p class="footer-section-title">INTRODUCTION</p> <ul> <li><a href="/wiki/Biomod/2013/Aarhus">Home, abstract, animation and video</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Introduction">Introduction</a></li </ul> </div> <div class="footer-section"> <p class="footer-section-title">RESULTS AND DISCUSSION</p> <ul> <li><a href="/wiki/Biomod/2013/Aarhus/Results_And_Discussion/Origami">Origami</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Results_And_Discussion/Peptide_lock">Peptide lock</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Results_And_Discussion/Chemical_Modification">Chemical modification</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Results_And_Discussion/sisiRNA">sisiRNA</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Results_And_Discussion/System_In_Action">System in action</a></li> </ul> </div> <div class="footer-section"> <p class="footer-section-title">MATERIALS AND METHODS</p> <ul> <li><a href="/wiki/Biomod/2013/Aarhus/Materials_And_Methods/Origami">Origami</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Materials_And_Methods/Peptide_lock">Peptide lock</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Materials_And_Methods/Chemical_Modification">Chemical modification</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Materials_And_Methods/sisiRNA">sisiRNA</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Materials_And_Methods/System_In_Action">System in action</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Materials_And_Methods/Methods">Methods</a></li> </ul> </div> <div class="footer-section"> <p class="footer-section-title">SUPPLEMENTARY</p> <ul> <li><a href="/wiki/Biomod/2013/Aarhus/Supplementary/Team_And_Acknowledgments">Team and acknowledgments</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Supplementary/Optimizations">Optimizations</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Supplementary/Supplementary_Data">Supplementary data</a></li> 

                                               <li><a

href="/wiki/Biomod/2013/Aarhus/Supplementary/Supplementary_Informations">Supplementary informations</a> <li><a href="/wiki/Biomod/2013/Aarhus/Supplementary/References">References</a></li> </ul> </div> </div> <div> <p id="copyright">Copyright (C) 2013 | BIOMOD Team Nano Creators @ Aarhus University | Programming by: <a href="mailto:pvskaarup@gmail.com?Subject=BIOMOD 2013:">Peter Vium Skaarup</a>.</p> </div> </div>

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