Biomod/2013/Aarhus/Supplementary/Supplementary Data: Difference between revisions
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[[image:sisi sup 3.png|950px|Cell viability corrected luciferase activities for all singly modified duplexes, transfected with Lipofectamine. Click on the image and choose 'high resolution' for details.]] | [[image:sisi sup 3.png|950px|Cell viability corrected luciferase activities for all singly modified duplexes, transfected with Lipofectamine. Click on the image and choose 'high resolution' for details.]] | ||
[[image:sisi supp 4.png|center| | [[image:sisi supp 4.png|center|thumb|950px|Best fit IC50 values of all knockdown experiments using singly modified duplexes using Lipofectamine.The IC50 value for W179-PEG1K was calculated with a low R2 value and is not conclusive.]] | ||
[[image:sisi supp 5.png|center| | [[image:sisi supp 5.png|center|thumb|950px|Luciferase and MTT measuremetns of all knockdown experiments with singly modified duplexes using Lipofectamine.]] | ||
===System in action=== | ===System in action=== |
Revision as of 13:08, 24 October 2013
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Supplementary data
Origami design
sisiRNA
Yields of RP-HPLC purifications of the modified strands
Estimated yields of the annealing reactions
Luciferase knockdown with all singly modified duplexes
System in action
Radioactive labeling of W376
The band containing the labeled sample appears most similar to that in lane 4 which means that the 6 µL that were run on the gel contained 1.5 pmol W376, corresponding to a concentration of 0.25 µM in the purified sample.
Cholesterol
1H NMR, 13C NMR and IR for compound 4.
1H NMR, 13C NMR and IR for compound 5.
1H NMR and 13C NMR for compound 6.
Photosensitizer
1H NMR and 13C NMR for compound 7.
1H NMR and 13C NMR for compound 8.
1H NMR and 13C NMR for compound 9.
1H NMR and 13C NMR for compound 10.
1H NMR and 13C NMR for compound 11.
1H NMR and 13C NMR for compound 12.
5-propargylamino-ddUTP
1H NMR, 13C NMR and IR for compound 15.
1H NMR, 13C NMR and IR for compound 16.
1H NMR, 13C NMR and IR for compound 17.
1H NMR, 13C NMR and IR for compound 18.
1H NMR and 19F NMR for compound 19.
1H NMR and 19F NMR for compound 21.
Peptide lock
Spectra
(S)-2-((tert-butoxycarbonyl)amino)-5-methoxy-5-oxopentanoic acid
1H NMR File:Exp4HNMRfinishedforrealz-eps-converted-to.pdf
13C NMR File:Exp4CNMRfinishedforrealz-eps-converted-to.pdf
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</style> </head> <body> <div id="indexing"> <div id="sitemap"> <p id="sitemapTitle">SITEMAP | BIOMOD 2013 NANO CREATORS | Aarhus University</p> <div id="footer-contents"> <div class="footer-section"> <p class="footer-section-title">INTRODUCTION</p> <ul> <li><a href="/wiki/Biomod/2013/Aarhus">Home, abstract, animation and video</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Introduction">Introduction</a></li </ul> </div> <div class="footer-section"> <p class="footer-section-title">RESULTS AND DISCUSSION</p> <ul> <li><a href="/wiki/Biomod/2013/Aarhus/Results_And_Discussion/Origami">Origami</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Results_And_Discussion/Peptide_lock">Peptide lock</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Results_And_Discussion/Chemical_Modification">Chemical modification</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Results_And_Discussion/sisiRNA">sisiRNA</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Results_And_Discussion/System_In_Action">System in action</a></li> </ul> </div> <div class="footer-section"> <p class="footer-section-title">MATERIALS AND METHODS</p> <ul> <li><a href="/wiki/Biomod/2013/Aarhus/Materials_And_Methods/Origami">Origami</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Materials_And_Methods/Peptide_lock">Peptide lock</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Materials_And_Methods/Chemical_Modification">Chemical modification</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Materials_And_Methods/sisiRNA">sisiRNA</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Materials_And_Methods/System_In_Action">System in action</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Materials_And_Methods/Methods">Methods</a></li> </ul> </div> <div class="footer-section"> <p class="footer-section-title">SUPPLEMENTARY</p> <ul> <li><a href="/wiki/Biomod/2013/Aarhus/Supplementary/Team_And_Acknowledgments">Team and acknowledgments</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Supplementary/Optimizations">Optimizations</a></li> <li><a href="/wiki/Biomod/2013/Aarhus/Supplementary/Supplementary_Data">Supplementary data</a></li>
<li><a
href="/wiki/Biomod/2013/Aarhus/Supplementary/Supplementary_Informations">Supplementary informations</a> <li><a href="/wiki/Biomod/2013/Aarhus/Supplementary/References">References</a></li> </ul> </div> </div> <div> <p id="copyright">Copyright (C) 2013 | BIOMOD Team Nano Creators @ Aarhus University | Programming by: <a href="mailto:pvskaarup@gmail.com?Subject=BIOMOD 2013:">Peter Vium Skaarup</a>.</p> </div> </div>
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