Biomod/2012/UTokyo/UT-Komaba/Experiment/Modified Origami
<html>
<style type="text/css"> <!--
- column-one {display:none; width:0px;}
- column-content {margin: 0;}
.container{background-color: #ffffff; margin-top:0px} .OWWNBcpCurrentDateFilled {display: none;}
- globalWrapper{margin: 0 auto; padding: 0; width: 900px;}
.firstHeading {display:none; width:0px;}
- content {margin-left: 0; padding: 0; border: none;}
- sidebar-main {display:none; width:0px;}
- footer{position: center; margin: 0;}
body {
margin: 0; font-family: Calibri, Verdana, helvetica, sans-serif; background: url(http://openwetware.org/images/2/21/Biomod-2012-ut-komaba-Metal-texture.jpg) darkgray repeat-y;
}
- content {
margin: 0;
}
/*title*/
- title{
margin: 10px 0; padding: auto; background-color: black; text-align :center;
}
- navi ul {
font-size: 12px; margin: 0 0 30px; padding: 0; text-align:center; height: 30px; background-color: black}
- navi ul li {
list-style-type: none; float: left; padding: 0; margin: 0; display: block}
- navi ul li a {
display: block; width: 100px; line-height: 30px; text-decoration: none; text-align: center; color: #ffffff; background-color: black;
}
- navi ul li a:hover {
background-color: gainsboro; color: black;
}
- bodyContent{
width: 800px;
margin: auto;
padding: 50px;
background: url("http://openwetware.org/images/c/c2/Biomod_2012_UToyko_UT-Komaba_background.png") white repeat-y;
font-size: 140%;
}
- toc{
font-size: 80%;
}
/* table */ table {
border: solid 1px black; border-collapse: collapse; border-spacing: 0;
}
table.noborder, table.noborder th, table.noborder td{
border: none;
}
table th{
border: solid 1px black; padding: 1px 5px; color: white; background-color: darkgray; text-align: center;
}
table td{
border: solid 1px black; padding: 1px 5px; text-align: center;
}
table.tdleft td{
text-align: left;
}
h1,h2,h3,h4 {
font-family: serif; clear: both;
}
table.dialog{
border: none;
}
table.dialog th{
border: none; padding-top: 0px; background-color: white; color: black; text-decolation: bold; text-align: left;
}
table.dialog td{
border: none; padding: 5px 0; text-align: left;
}
--> </style>
<div id="title"><img src="http://openwetware.org/images/4/47/Biomod_2012_UTokyo_UT-Komaba_Top.png" alt="DNA tablet" width="800" height="120" onClick="this.src='http://openwetware.org/images/7/7d/BIOMOD_2012_UTokyo_UT-Komaba_title-animation.gif'"/></div>
<div id="navi"> <ul>
<li><a href="/wiki/Biomod/2012/UTokyo/UT-Komaba">Home</a></li> <li><a href="/wiki/Biomod/2012/UTokyo/UT-Komaba/Idea">Idea</a></li> <li><a href="/wiki/Biomod/2012/UTokyo/UT-Komaba/Simulation">Simulation</a></li> <li><a href="/wiki/Biomod/2012/UTokyo/UT-Komaba/Experiment">Experiment</a></li> <li><a href="/wiki/Biomod/2012/UTokyo/UT-Komaba/Progress">Progress</a></li> <li><a href="/wiki/Biomod/2012/UTokyo/UT-Komaba/Episode">Episode</a></li> <li><a href="/wiki/Biomod/2012/UTokyo/UT-Komaba/Team">Team</a></li> <li><a href="/wiki/Biomod/2012/UTokyo/UT-Komaba/Supplementary">Supplementary</a></li>
</ul> </div>
</html>
Concept
We combine DNA origami and hammerhead structure so that, when cover DNAs hybridize to the structure, the surface of the modified origami show a picture. Therefore, if we can set up the bistable system so that it produces the cover DNAs for different images, we can realize the DNA tablet. The purpose of the experiment is to make sure that the hammerhead structure DNAs do not disturb during annealing in PCR. We observe the modified DNA origami with cover DNA by AFM.
