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==September 24th== | ==September 24th== |
Revision as of 15:12, 21 October 2012
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Lab Notes
We conducted a lot of experiments at Komaba Research Campus.
September 12th
DNA Origami
We made a solution of DNA origami.
(µL)
M13 | Staple Mix | TAE 10x | Mg2+ | mQ | Total Amount | |
---|---|---|---|---|---|---|
A | 3.00 | 7.20 | 1.80 | 2.25 | 3.75 | 20.00 |
B | 3.00 | 3.60 | 1.80 | 2.25 | 7.35 | 20.00 |
C | 3.00 | 10.80 | 1.80 | 2.25 | 0.15 | 20.00 |
September 13th
DNA Origami
We observed DNA origami which we made September 12th by AFM.
- AFM image of A
- AFM image of C
September 19th
DNA Origami
We prepared two types of DNA origami solution. The purpose of the experiment was to compare the different result from the different concentration of the solutions.
(µL)
Staple Mix | M13 | TAE 10x | Mg2+ | mQ | Total Amount | |
---|---|---|---|---|---|---|
A | 8.00 | 3.33 | 2.00 | 0.25 | 6.42 | 20.00 |
B | 12.00 | 5.00 | 2.00 | 0.25 | 0.75 | 20.00 |
DNA Origami with Enzymes
We use enzymes in bistable system, therefore we make sure that the enzymes do not destroy the DNA origami. We put nickase, polymerase and exnuclease with DNA origami and left them in the PCR. The concentration of original DNA origami was 10x and 15x.
(µL)
BST | NBI | tt-RecJ | DTT | BSA | Smix | DNA origami | mQ | Total Amount | |
---|---|---|---|---|---|---|---|---|---|
DNA origami 10x | 0.20 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 10.00 | 3.30 | 20.00 |
DNA origami 15x | 0.20 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 10.00 | 3.30 | 20.00 |
DNA Origami with BSA
We are also worried if BSA interrupt the AFM and we can not get clear images of DNA origami. Therefore, we prepare the solution for September 20th. The concentration of original DNA origami was 10x and 15x.
(µL)
DNA origami | Smix 4x | BSA | mQ | Total Amount | |
---|---|---|---|---|---|
DNA origami 10x | 2.00 | 5.00 | 2.00 | 11.00 | 20.00 |
DNA origami 15x | 2.00 | 5.00 | 2.00 | 11.00 | 20.00 |
Bistable System
We wanted to know the best concentration for the bistable system.
Two different DNA strands, "XII" and "VII", were used for the experiment.
We searched the best concentration of CvII and CxII separately.
CvII is the template for doubling V, and CxII is the one of X.
"X to inhV" is the template for making inhV DNA from X, and "V to inhX" is the one of V.
We change the concentration of CvII and CxII in order to find out the best concentration of them.
We keep PCR in 42°C.
- The Experiment of VII
(µL)
Bst | NBI | tt-RecJ | DTT | BSA | Smix 4x | V to inhX | X to inhV | dTTP | VII | CvII | mQ | Total Amount | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
5x | 0.21 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.42 | 0.21 | 0.11 | 0.11 | 12.69 | 21.00 |
10x | 0.21 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.42 | 0.21 | 0.11 | 0.21 | 12.59 | 21.00 |
15x | 0.21 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.42 | 0.21 | 0.11 | 0.32 | 12.48 | 21.00 |
20x | 0.21 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.42 | 0.21 | 0.11 | 0.43 | 12.37 | 21.00 |
- The Experiment of XII
(µL)
Bst | NBI | tt-RecJ | DTT | BSA | Smix 4x | V to inhX | X to inhV | dTTP | XII | CxII | mQ | Total Amount | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
5x | 0.21 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.42 | 0.21 | 0.11 | 0.11 | 12.69 | 21.00 |
10x | 0.21 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.42 | 0.21 | 0.11 | 0.21 | 12.59 | 21.00 |
15x | 0.21 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.42 | 0.21 | 0.11 | 0.32 | 12.48 | 21.00 |
20x | 0.21 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.42 | 0.21 | 0.11 | 0.43 | 12.37 | 21.00 |
When several hours passed after keeping these tubes in 42°C, we injected XII 0.40µL in each VII tubes and VII 0.40µL in each XII tubes.
Then we kept these tubes in 42°C for about 1 day.
September 20th
DNA origami
September 24th
Bistable System
We change the condition of the bistable experiment and put some tubes in PCR. The concentration of Bst and X to inhV were different from the same experiment of September 19th. We kept these tubes in 42°C.
- The Experiment of VII
(µL)
Bst | NBI | tt-RecJ | dTTP | BSA | Smix 4x | V to inhX | X to inhV | DTTP | VII | CvII | mQ | Total Amount | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
5x | 0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.11 | 12.83 | 21.00 |
10x | 0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.21 | 12.73 | 21.00 |
15x | 0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.32 | 12.62 | 21.00 |
20x | 0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.43 | 12.51 | 21.00 |
- The Experiment of XII
(µL)
Bst | NBI | tt-RecJ | DTT | BSA | Smix 4x | V to inhX | X to inhV | dTTP | XII | CxII | mQ | Total Amount | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
5x | 0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.11 | 12.83 | 21.00 |
10x | 0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.21 | 12.73 | 21.00 |
15x | 0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.32 | 12.62 | 21.00 |
20x | 0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.43 | 12.51 | 21.00 |
When several hours had passed since we put them in PCR, we injected XII 0.40µL in each VII tubes and VII 0.40µL in each XII tubes.
Then we kept these tubes in 42°C for about 1 day as the bistable experiment we did before.
September 28th
Indirect Bistable System
We could not get a good result of the bistable system so that we change several part of the system. We introduce two types of DNA, "DII" and "NII", and two types of templates, "D to V" and "N to X" in the system. We change the concentration of "D to V" and "N to X", which are always the saame concentration. We also kept these tubes in 42°C.
- The Experiment of XII
(µL)
BST | NBI | tt-RecJ | DTT | BSA | Smix 4x | V to inhX | X to inhv | dTTP | XII | CxII | CvII | D to V | N to X | mQ | Total Amount | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2.5nM | 0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.11 | 0.42 | 0.05 | 0.05 | 12.20 | 21.00 |
5.0nM | 0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.11 | 0.42 | 0.11 | 0.11 | 12.08 | 21.00 |
7.5nM | 0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.11 | 0.42 | 0.16 | 0.16 | 11.98 | 21.00 |
10.0nM | 0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.11 | 0.42 | 0.21 | 0.21 | 11.88 | 21.00 |
- The Experiment of VII
(µL)
BST | NBI | tt-RecJ | DTT | BSA | Smix 4x | V to inhX | X to inhv | dTTP | VII | CxII | CvII | D to V | N to X | mQ | Total Amount | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2.5nM | 0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.11 | 0.42 | 0.05 | 0.05 | 12.20 | 21.00 |
5.0nM | 0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.11 | 0.42 | 0.11 | 0.11 | 12.08 | 21.00 |
7.5nM | 0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.11 | 0.42 | 0.16 | 0.16 | 11.98 | 21.00 |
10.0nM | 0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.11 | 0.42 | 0.21 | 0.21 | 11.88 | 21.00 |
When several hours had passed since we put these tubes in PCR, we put DII in tubes of X and NII in that of V so that the concentration of X or V might not change radically.
After putting DNAs, we kept them in PCR.
October 1st
Indirect Bistable System
We conducted the same experiment as what we did in September 28th. We got enough information about the experiment of VII, so we only conducted that of VII. The purpose of the experiment is to find out the best condition of the experiment of XII. We changed the concentration of CvII and X to inhV and compared the results.
- The Concentration of X to inhV: 10.00nM.
(µL)
BST | NBI | tt-RecJ | DTT | BSA | Smix 4x | V to inhX | X to inhv | dTTP | XII | CxII | CvII | D to V | N to X | mQ | Total Amount | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CvII: 20.00nM | 0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.20 | 0.20 | 0.10 | 0.10 | 0.40 | 0.20 | 0.20 | 11.54 | 20.00 |
CvII: 22.50nM | 0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.20 | 0.20 | 0.10 | 0.10 | 0.45 | 0.20 | 0.20 | 11.49 | 20.00 |
CvII: 25.00nM | 0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.20 | 0.20 | 0.10 | 0.10 | 0.50 | 0.20 | 0.20 | 11.44 | 20.00 |
- The Concentration of X to inhV: 15.00nM
(µL)
BST | NBI | tt-RecJ | DTT | BSA | Smix 4x | V to inhX | X to inhv | dTTP | XII | CxII | CvII | D to V | N to X | mQ | Total Amount | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CvII: 20.00nM | 0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.30 | 0.20 | 0.10 | 0.10 | 0.40 | 0.20 | 0.20 | 11.44 | 20.00 |
CvII: 22.50nM | 0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.30 | 0.20 | 0.10 | 0.10 | 0.45 | 0.20 | 0.20 | 11.39 | 20.00 |
CvII: 25.00nM | 0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.30 | 0.20 | 0.10 | 0.10 | 0.50 | 0.20 | 0.20 | 11.34 | 20.00 |
October 2nd
Bistable System
We lowered the concentratinons of D to V, and N to X. Today, we conducted experiments in order to find out the ideal concentration of "X to inhV". We kept these tubes in 42°C, and injected once each wells.
- The Experiment of XII
(µL)
BST | NBI | tt-RecJ | DTT | BSA | Smix 4x | V to inhX | X to inhv | dTTP | XII | CxII | CvII | mQ | Total Amount |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.21 | 0.21 | 0.11 | 0.11 | 0.42 | 12.54 | 21 |
0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.25 | 0.21 | 0.11 | 0.11 | 0.42 | 12.50 | 21 |
0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.28 | 0.21 | 0.11 | 0.11 | 0.42 | 12.47 | 21 |
0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.11 | 0.42 | 12.43 | 21 |
injection: VII 0.6µL each
- The Experiment of VII
(µL)
BST | NBI | tt-RecJ | DTT | BSA | Smix 4x | V to inhX | X to inhv | dTTP | VII | CxII | CvII | mQ | Total Amount |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.21 | 0.21 | 0.11 | 0.11 | 0.42 | 12.54 | 21 |
0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.25 | 0.21 | 0.11 | 0.11 | 0.42 | 12.50 | 21 |
0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.28 | 0.21 | 0.11 | 0.11 | 0.42 | 12.47 | 21 |
0.17 | 0.84 | 0.32 | 0.21 | 0.21 | 5.25 | 0.42 | 0.32 | 0.21 | 0.11 | 0.11 | 0.42 | 12.43 | 21 |
injection: XII 0.6µL each
Trioscillate System
To investigate ideal condition of trioscillate system, we conducted experiments which checks three parts of this system.
(µL)
BST | NBI | tt-RecJ | DTT | BSA | Smix 4x | inhmix | dTTP | XII | CxII | mQ | Total Amount |
---|---|---|---|---|---|---|---|---|---|---|---|
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.50 | 0.20 | 0.10 | 0.10 | 12.44 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.50 | 0.20 | 0.10 | 0.20 | 12.34 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.50 | 0.20 | 0.10 | 0.30 | 12.24 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.50 | 0.20 | 0.10 | 0.40 | 12.14 | 20.00 |
injection: VII 0.80µL each
(µL)
BST | NBI | tt-RecJ | DTT | BSA | Smix 4x | inhmix | dTTP | VII | CvII | mQ | Total Amount |
---|---|---|---|---|---|---|---|---|---|---|---|
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.50 | 0.20 | 0.10 | 0.10 | 12.44 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.50 | 0.20 | 0.10 | 0.20 | 12.34 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.50 | 0.20 | 0.10 | 0.30 | 12.24 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.50 | 0.20 | 0.10 | 0.40 | 12.14 | 20.00 |
injection: QII 0.80µL each
(µL)
BST | NBI | tt-RecJ | DTT | BSA | Smix 4x | inhmix | dTTP | QII | CqII | mQ | Total Amount |
---|---|---|---|---|---|---|---|---|---|---|---|
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.50 | 0.20 | 0.10 | 0.10 | 12.44 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.50 | 0.20 | 0.10 | 0.20 | 12.34 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.50 | 0.20 | 0.10 | 0.30 | 12.24 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.50 | 0.20 | 0.10 | 0.40 | 12.14 | 20.00 |
injection: XII 0.80µL each
October 3rd
Bistable System
In today's experiment, we investigated the ideal temperature for the bistable system. We made two solutions (one starts from "X", and the other from "Y"), and observed at 6 different temperatures - 41.6~47.6℃.
(µL)
BST | NBI | tt-RecJ | DTT | BSA | Smix 4x | D to V,N to X | V to inhX | X to inhv | dTTP | XII | VII | CxII | CvII | mQ | Total Amount |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 1.50 | 0.40 | 0.30 | 0.20 | 0.10 | 0.00 | 0.10 | 0.40 | 10.34 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 1.50 | 0.40 | 0.30 | 0.20 | 0.00 | 0.10 | 0.10 | 0.40 | 10.34 | 20.00 |
injection: XII or VII 0.60µL each
October 11th
Trioscillate system
Today, we tested whole trioscillate system. To investigate the ideal condition, we tested both direction of inhibition (V-|X-|Q, V-|Q-|X) and each concentration of CxII, CvII, CqII.
- inhibit direction: V-|X-|Q
(µL)
BST | NBI | tt-RecJ | DTT | BSA | Smix 4x | inhmix | dTTP | XII | VII | CxII | CvII | CqII | mQ | Total Amount |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.50 | 0.40 | 0.50 | 11.22 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.50 | 0.40 | 1.00 | 10.72 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.50 | 0.40 | 1.50 | 10.22 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.50 | 0.40 | 2.00 | 9.72 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 1.00 | 0.40 | 0.50 | 10.72 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 1.00 | 0.40 | 1.00 | 10.22 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 1.00 | 0.40 | 1.50 | 9.72 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 1.00 | 0.40 | 2.00 | 8.22 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 1.50 | 0.40 | 0.50 | 10.22 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 1.50 | 0.40 | 1.00 | 9.72 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 1.50 | 0.40 | 1.50 | 9.22 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 1.50 | 0.40 | 2.00 | 8.72 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 2.00 | 0.40 | 0.50 | 9.72 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 2.00 | 0.40 | 1.00 | 9.22 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 2.00 | 0.40 | 1.50 | 8.72 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 2.00 | 0.40 | 2.00 | 8.22 | 20.00 |
- inhibit direction: V-|Q-|X
(µL)
BST | NBI | tt-RecJ | DTT | BSA | Smix 4x | inhmix | dTTP | XII | VII | CxII | CvII | CqII | mQ | Total Amount |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.10 | 0.50 | 0.50 | 11.52 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.10 | 0.50 | 1.00 | 11.02 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.10 | 0.50 | 1.50 | 10.52 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.10 | 0.50 | 2.00 | 10.02 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.10 | 1.00 | 0.50 | 11.02 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.10 | 1.00 | 1.00 | 10.52 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.10 | 1.00 | 1.50 | 10.02 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.10 | 1.00 | 2.00 | 9.52 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.10 | 1.50 | 0.50 | 10.52 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.10 | 1.50 | 1.00 | 10.02 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.10 | 1.50 | 1.50 | 9.52 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.10 | 1.50 | 2.00 | 9.02 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.10 | 2.00 | 0.50 | 10.02 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.10 | 2.00 | 1.00 | 9.52 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.10 | 2.00 | 1.50 | 9.02 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.10 | 2.00 | 2.00 | 8.52 | 20.00 |
October 12th
Trioscillate system
We tested again the wells which worked well in before experiment.
- inhibit direction: V-|X-|Q
(µL)
BST | NBI | tt-RecJ | DTT | BSA | Smix 4x | inhmix | dTTP | XII | VII | CxII | CvII | CqII | mQ | Total Amount |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.50 | 0.40 | 0.50 | 11.22 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.50 | 0.40 | 1.00 | 10.72 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.50 | 0.40 | 1.50 | 10.22 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 0.50 | 0.40 | 2.00 | 9.72 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 1.00 | 0.40 | 0.50 | 10.72 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 1.00 | 0.40 | 1.00 | 10.22 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 1.00 | 0.40 | 1.50 | 9.72 | 20.00 |
0.16 | 0.80 | 0.30 | 0.20 | 0.20 | 5.00 | 0.40 | 0.10 | 0.11 | 0.11 | 1.00 | 0.40 | 2.00 | 8.22 | 20.00 |