Biomod/2012/UCSD/tRiton Nano Architects/Project introduction: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 1: Line 1:
{{Biomod/2012/UCSD/UCSDtemplate}}
{{Biomod/2012/UCSD/UCSDtemplate}}


=Motivation=
=Concepts=


Why did we do this project?
==What is an Aptamer?==


===Summer Goals===
An aptamer is a stable DNA or RNA molecule that has been selected from random pools based on it’s ability to bind with high affinity to target molecules such as small organics, proteins, and even entire cells. Unlike antibodies, aptamers are selected in vitro, are capable of higher specificity and affinity, can tolerate pH and temperatures that their protein counterparts cannot. These features allow them to be highly specific detectors and to be stored at ambient temperatures with much higher shelf life.


What was our goals in this project for the summer?
==References==


=Future Expectations=
1. Stoltenburg R, Reinemann C, Strehlitz B: SELEX–a (r)evolutionary method to generate high-affinity nucleic acid ligands. Biomolecular engineering 2007, 24:381-403.
 
2. Jayasena SD: Aptamers: An Emerging Class of Molecules That Rival Antibodies in Diagnostics. Clinical Chemistry 1999, 45:1628-1650.


Coming Soon




Revision as of 23:04, 27 October 2012

<html> <head> 

 <style>
  1. column-one { display:none; width:0px;}

.container{background-color: #ececec; margin-top:50px} .OWWNBcpCurrentDateFilled {display: none;}

  1. content {width: 0px; margin: 0 auto auto 0; padding: 1em 1em 1em 1em; align: center;}
  2. column-content {width: 0px; float: left; margin: 0 0 0 0;padding: 0;}

.firstHeading {display:none; width:0px;}

  1. globalWrapper{width:1280px; margin:auto}

body {background: #ececec!important;}

  1. column-one {display:none; width:0px;background-color: #ececec;}
  2. content{border:none;margin: 0 0 0 0; padding: 1em 1em 1em 1em; position: center; width: 800px;background-color: #ececec; }

.container{ width: 800px; margin: auto; background-color: #ececec; text-align:justify; font-family: helvetica, arial, sans-serif; color:#ececec; margin-top:25px; }

  1. bodyContent{ width: 1267px; align: center; background-color: #ececec;}
  2. column-content{width: 1280px;background-color: #ececec;}

.firstHeading { display:none;width:0px;background-color: #ececec;}

  1. header{position: center; width: 800px;background-color: #ececec;}
  2. footer{position: center; width:1280px;}
 </style>

</head> </html>

<html><center><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects'><img src=http://openwetware.org/images/a/a0/Trna_logo_with_title5.png width=1118px></a></center></html> <html><center><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects'><img src=http://openwetware.org/images/e/e5/Menuspacer2.png width=8px></a></center></html>

<html> <head> 

 <style type="text/css">

@import url(http://fonts.googleapis.com/css?family=Open+Sans:600);

/* Menu CSS */

  1. cssmenu, #cssmenu > ul{

background: url(http://openwetware.org/images/a/a0/Highlight-bg.png) repeat; padding-bottom: 3px; font-family: 'Open Sans', sans-serif; font-weight: 600;

}

  1. cssmenu:before, #cssmenu:after, #cssmenu > ul:before, #cssmenu > ul:after {

content: ''; display: table;

}


  1. cssmenu:after, #cssmenu > ul:after {

clear: both;

}

  1. cssmenu {

zoom:1;

}

  1. cssmenu > ul{

background: url(http://openwetware.org/images/0/04/Menu-bg2.png) repeat; margin: 0; padding: 0; position: relative;

}

  1. cssmenu > ul li{

margin: 0; padding: 0; list-style: none;

}

  1. cssmenu > ul > li{

float: left; position: relative;

}

  1. cssmenu > ul > li > a{

padding: 23px 26px; display: block; color: white; font-size: 13px; text-decoration: none; text-transform: uppercase; text-shadow: 0 -1px 0 #9e3825; text-shadow: 0 -1px 0 rgba(10, 12, 42, .70); line-height: 18px;

}


  1. cssmenu > ul > li:hover > a{

background: url(http://openwetware.org/images/4/48/Hover5.png) repeat; text-shadow: 0 -1px 0 #97321f; text-shadow: 0 -1px 0 rgba(32, 56, 112, .64);

}

  1. cssmenu > ul > li > a > span{

line-height: 18px;

}

  1. cssmenu > ul > li.active > a, #cssmenu > ul > li > a:active{

background: url(http://openwetware.org/images/b/b1/Active3.png) repeat;

}


/* Childs */

  1. cssmenu > ul ul{

opacity: 0; visibility: hidden; position: absolute; top: 120px; background: url(http://openwetware.org/images/a/a0/Highlight-bg.png) repeat; margin: 0; padding: 0; z-index: -1; -webkit-transition: all .35s .2s ease-in-out; -moz-transition: all .35s .2s ease-in-out; -ms-transition: all .35s .2s ease-in-out; transition: all .35s .2s ease-in-out;

}

  1. cssmenu > ul li:hover ul{

opacity: 1; visibility: visible; margin: 0; color: #000; z-index: 2; top:64px; left: 0;

}

  1. cssmenu > ul ul:before{

content: ''; position: absolute; top: -10px; width: 100%; height: 20px; background: transparent;

}


  1. cssmenu > ul ul li{

list-style: none; padding: 0; margin: 0; width: 100%;

}

  1. cssmenu > ul ul li a{

padding: 18px 26px; display: block; color: #393939; font-size: 13px; text-decoration: none; text-transform: uppercase; width: 150px; border-left: 4px solid transparent; -webkit-transition: all .35s ease-in-out; -moz-transition: all .35s ease-in-out; -ms-transition: all .35s ease-in-out; transition: all .35s ease-in-out; text-shadow: 0 1px 0 white;

}

  1. cssmenu > ul ul li a:hover{

border-left: 4px solid #0f2858; background: url(http://openwetware.org/images/a/a0/Highlight-bg.png) repeat; color: #20295C; text-shadow: 0 1px 0 black;

}

  1. cssmenu > ul ul li a:active{

background: url(http://openwetware.org/images/0/04/Menu-bg2.png) repeat;

} 

 </style type="text/css">

<div id='cssmenu'> <ul> 

  <li class='active has-sub '><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects'><span>Home</span></a>
     <ul>
        <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects#Abstract:_Fluorescent_DNA_Aptamer_for_lysozyme_detection'><span>Abstract</span></a></li>
        <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects#Video'><span>Video</span></a></li>
     </ul>
  </li>
  <li class='has-sub '><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Project_introduction'><span>Project Introduction</span></a>
     <ul>
        <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Project_introduction#Concepts'><span>Concepts</span></a></li>
     </ul>
  </li>
  <li class='has-sub '><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Experiment'><span>Experiment</span></a>
     <ul>
        <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Experiment#Methods'><span>Methods</span></a></li>
     </ul>
  </li>
  <li class='has-sub '><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Resources'><span>Supplementary Resources</span></a>
     <ul>
        <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Brainstorm#DNA_architecture_log'><span>DNA Architecture Log</span></a></li>
        <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Brainstorm#Project_Ideas'><span>Project Ideas</span></a></li>
        <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Literature'><span>Literature</span></a></li>
     </ul>
  </li>
  <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Team'><span>Team</span></a></li>

</ul> </div>

</head> </html>

<html><center><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects'><img src=http://openwetware.org/images/a/ac/School.png width=1126px></a></center></html>

Concepts

What is an Aptamer?

An aptamer is a stable DNA or RNA molecule that has been selected from random pools based on it’s ability to bind with high affinity to target molecules such as small organics, proteins, and even entire cells. Unlike antibodies, aptamers are selected in vitro, are capable of higher specificity and affinity, can tolerate pH and temperatures that their protein counterparts cannot. These features allow them to be highly specific detectors and to be stored at ambient temperatures with much higher shelf life.

References

1. Stoltenburg R, Reinemann C, Strehlitz B: SELEX–a (r)evolutionary method to generate high-affinity nucleic acid ligands. Biomolecular engineering 2007, 24:381-403.

2. Jayasena SD: Aptamers: An Emerging Class of Molecules That Rival Antibodies in Diagnostics. Clinical Chemistry 1999, 45:1628-1650.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Biomod/2012/UCSD/tRiton_Nano_Architects/Project_introduction&oldid=648406"