Biomod/2012/Tohoku/Team Sendai - Revision history

Kazuki Hirahara at 02:43, 28 October 2012

2012-10-28T02:43:29Z

←Older revision		Revision as of 02:43, 28 October 2012
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	{{:Biomod/2012/Tohoku/Team_Sendai/header}}		{{:Biomod/2012/Tohoku/Team_Sendai/header}}
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Kazuki Hirahara at 00:01, 28 October 2012

2012-10-28T00:01:08Z

←Older revision		Revision as of 00:01, 28 October 2012
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	<area shape="rect" coords="310,8,390,45" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER" alt="Porter">		<area shape="rect" coords="310,8,390,45" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER" alt="Porter">
	<area shape="rect" coords="310,95,370,132" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE" alt="Gate">		<area shape="rect" coords="310,95,370,132" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE" alt="Gate">
-	<area shape="rect" coords="33,213,165,245" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#~~MEMBRANEl~~" alt="Membarane">	+	<area shape="rect" coords="33,213,165,245" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE" alt="Membarane">
	</map>		</map>
	Please CLICK ITEMS, GATE, PORTER, AND MEMBRANE in the picture!		Please CLICK ITEMS, GATE, PORTER, AND MEMBRANE in the picture!

Kazuki Hirahara at 22:29, 27 October 2012

2012-10-27T22:29:49Z

←Older revision		Revision as of 22:29, 27 October 2012
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	<area shape="rect" coords="33,213,165,245" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANEl" alt="Membarane">		<area shape="rect" coords="33,213,165,245" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANEl" alt="Membarane">
	</map>		</map>
		+	Please CLICK ITEMS, GATE, PORTER, AND MEMBRANE in the picture!
	</div>		</div>

Kazuki Hirahara at 20:42, 27 October 2012

2012-10-27T20:42:47Z

←Older revision		Revision as of 20:42, 27 October 2012
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-	~~<p>~~	+
-	~~<a href=" http://openwetware.org/wiki/Biomod/2012/Team_Sendai_Old">test</a>~~	+
-	~~</p>~~	+
	</div>		</div>

	</body>		</body>
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Kazuki Hirahara at 19:18, 27 October 2012

2012-10-27T19:18:29Z

←Older revision		Revision as of 19:18, 27 October 2012
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	<p>		<p>
	</br>		</br>
-	<iframe width="420" height="315" src="http://www.youtube.com/embed/~~9k-Xpl3sRCQ~~" frameborder="0" allowfullscreen></iframe>	+	<iframe width="420" height="315" src="http://www.youtube.com/embed/8OB8ytltxwY" frameborder="0" allowfullscreen></iframe>
	</p>		</p>
	</div>		</div>

Kazuki Hirahara at 17:25, 27 October 2012

2012-10-27T17:25:32Z

←Older revision		Revision as of 17:25, 27 October 2012
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	A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.</br>		A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.</br>
	Here, we decided to create a novel device with such a function. </br>		Here, we decided to create a novel device with such a function. </br>
-	We have designed a cylindrical injector/extractor device made of DNA origami (we named this device: CELL-GATE). Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.</br>	+	We have designed <strong>a cylindrical injector/extractor device made of DNA origami</strong> (we named this device: <strong>CELL-GATE</strong>). Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.</br>
	We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.</br>		We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.</br>
	</p>		</p>
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	<p>		<p>
	<strong>Our PROJECT CELL-GATE is divided into three sub-projects:</strong><br>		<strong>Our PROJECT CELL-GATE is divided into three sub-projects:</strong><br>
-	<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a>: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called Porter. ~~Once the outer-most Porter binds~~ to a target DNA, the ~~target is passed to the inner ones one by one~~.</br>	+	<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a>: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called Porter. Porters are designed to transfer target DNA strands into (or out from) the membrane.</br>
-	<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a>: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a ~~gate~~. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.</br>	+	<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a>: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a channel. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.</br>
	<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.		<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.
	</p>		</p>

Kazuki Hirahara at 16:19, 27 October 2012

2012-10-27T16:19:46Z

←Older revision		Revision as of 16:19, 27 October 2012
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	<p>		<p>
	<strong>Our PROJECT CELL-GATE is divided into three sub-projects:</strong><br>		<strong>Our PROJECT CELL-GATE is divided into three sub-projects:</strong><br>
-	~~<b>~~<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a~~></b~~>: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called Porter. Once the outer-most Porter binds to a target DNA, the target is passed to the inner ones one by one.</br>	+	<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a>: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called Porter. Once the outer-most Porter binds to a target DNA, the target is passed to the inner ones one by one.</br>
-	~~<b>~~<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a~~></b~~>: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a gate. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.</br>	+	<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a>: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a gate. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.</br>
-	~~<b>~~<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a~~></b~~>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.	+	<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.
	</p>		</p>

Kazuki Hirahara at 16:18, 27 October 2012

2012-10-27T16:18:27Z

←Older revision		Revision as of 16:18, 27 October 2012
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	A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.</br>		A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.</br>
	Here, we decided to create a novel device with such a function. </br>		Here, we decided to create a novel device with such a function. </br>
-	We have designed a ~~<b>~~cylindrical injector/extractor device made of DNA origami (we named this device: CELL-GATE)~~</b>~~. Inside the cylinder, a cascade of single stranded DNAs ~~(ssDNA)~~ is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.</br>	+	We have designed a cylindrical injector/extractor device made of DNA origami (we named this device: CELL-GATE). Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.</br>
	We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.</br>		We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.</br>
	</p>		</p>
		+


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	<h2><!--Abstract-->Project Image</h2>		<h2><!--Abstract-->Project Image</h2>
	<p>		<p>
-	<strong>~~We divided~~ CELL-GATE into three sub-projects ~~with the following functions~~:</strong> ~~(Please click to see more details)~~ <br>	+	<strong>Our PROJECT CELL-GATE is divided into three sub-projects:</strong><br>
-	<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a></b>: ~~Cascade~~ of ssDNA ~~capable of capturing & transporting~~ a target DNA <br>	+	<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a></b>: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called Porter. Once the outer-most Porter binds to a target DNA, the target is passed to the inner ones one by one.</br>
-	<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a></b>: ~~Nanostructural channel made of~~ DNA ~~origami that includes PORTER~~ inside. ~~GATE is inserted in~~ the ~~cell membrane~~ <br>	+	<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a></b>: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a gate. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.</br>
-	<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a></b>: We use liposome as model ~~for the cell~~ membrane.	+	<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a></b>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.
	</p>		</p>
-	<img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px"><br>	+
		+	<img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px" usemap="#D-Heart">
		+	<map name="D-Heart">
		+	<area shape="rect" coords="310,8,390,45" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER" alt="Porter">
		+	<area shape="rect" coords="310,95,370,132" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE" alt="Gate">
		+	<area shape="rect" coords="33,213,165,245" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANEl" alt="Membarane">
		+	</map>
	</div>		</div>

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	<br clear = "left">		<br clear = "left">

		+
		+	<p>
		+	<a href=" http://openwetware.org/wiki/Biomod/2012/Team_Sendai_Old">test</a>
		+	</p>
	</div>		</div>

	</body>		</body>
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Keitel Cervantes Salguero at 15:43, 27 October 2012

2012-10-27T15:43:30Z

←Older revision		Revision as of 15:43, 27 October 2012
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	<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a></b>: Cascade of ssDNA capable of capturing & transporting a target DNA <br>		<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a></b>: Cascade of ssDNA capable of capturing & transporting a target DNA <br>
	<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a></b>: Nanostructural channel made of DNA origami that includes PORTER inside. GATE is inserted in the cell membrane <br>		<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a></b>: Nanostructural channel made of DNA origami that includes PORTER inside. GATE is inserted in the cell membrane <br>
-	<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a></b>: We use liposome as model for cell membrane.	+	<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a></b>: We use liposome as model for the cell membrane.
	</p>		</p>
	<img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px"><br>		<img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px"><br>

Keitel Cervantes Salguero at 15:42, 27 October 2012

2012-10-27T15:42:36Z

←Older revision		Revision as of 15:42, 27 October 2012
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	A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.</br>		A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.</br>
	Here, we decided to create a novel device with such a function. </br>		Here, we decided to create a novel device with such a function. </br>
-	We have designed a <b>cylindrical injector/extractor device made of DNA origami (we named this device: CELL-GATE)</b>. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.</br>	+	We have designed a <b>cylindrical injector/extractor device made of DNA origami (we named this device: CELL-GATE)</b>. Inside the cylinder, a cascade of single stranded DNAs (ssDNA) is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.</br>
	We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.</br>		We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.</br>
	</p>		</p>
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	<div id="pop">		<div id="pop">
	<h2><!--Abstract-->Project Image</h2>		<h2><!--Abstract-->Project Image</h2>
-	~~<img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px" usemap="#D-Heart">~~
-	~~<map name="D-Heart">~~
-	~~<area shape="rect" coords="310,8,390,45" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER" alt="Porter">~~
-	~~<area shape="rect" coords="310,95,370,132" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE" alt="Gate">~~
-	~~<area shape="rect" coords="33,213,165,245" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANEl" alt="Membarane">~~
-	~~</map>~~
-
	<p>		<p>
-	<strong>~~Our PROJECT~~ CELL-GATE ~~is divided~~ into three sub-projects:</strong><br>	+	<strong>We divided CELL-GATE into three sub-projects with the following functions:</strong> (Please click to see more details) <br>
-	<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a></b>: ~~Composed~~ of ~~single stranded DNA (~~ssDNA~~) sequences. Each ssDNA is called Porter. Once the outer-most Porter binds to~~ a target DNA~~, the target is passed to the inner ones one by one.~~</br>	+	<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a></b>: Cascade of ssDNA capable of capturing & transporting a target DNA <br>
-	<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a></b>: ~~Cylindrical~~ DNA ~~nanostructure connecting the~~ inside ~~and outside of membrane like a gate~~. ~~A cascade of~~ the ~~Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.~~</br>	+	<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a></b>: Nanostructural channel made of DNA origami that includes PORTER inside. GATE is inserted in the cell membrane <br>
-	<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a></b>: ~~The exterior and the interior of the cell are compartmentalized by biomembranes.~~ We use liposome as a model membrane.	+	<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a></b>: We use liposome as model for cell membrane.
	</p>		</p>
		+	<img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px"><br>
	</div>		</div>

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-
-	~~<p>~~
-	~~<a href=" http://openwetware.org/wiki/Biomod/2012/Team_Sendai_Old">test</a>~~
-	~~</p>~~
	</div>		</div>

	</body>		</body>
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