Biomod/2012/Tohoku/Team Sendai

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<h1 align="center";><!--Team Sendai--><img src="http://openwetware.org/images/c/c2/Title-kai.png" ></h1>
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<h1>Abstract</h1>
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All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes.</br>
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A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.</br>
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Here, we decided to create a novel device with such a function. </br>
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We have designed <strong>a cylindrical injector/extractor device made of DNA origami</strong> (we named this device: <strong>CELL-GATE</strong>). Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.</br>
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We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.</br>
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<strong>Our PROJECT CELL-GATE is divided into three sub-projects:</strong><br>
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<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a>: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called  Porter. Porters are designed to transfer target DNA strands into (or out from) the membrane.</br>
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<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a>: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a channel. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.</br>
 +
<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.
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<area shape="rect" coords="310,8,390,45" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER" alt="Porter">
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<area shape="rect" coords="310,95,370,132" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE" alt="Gate">
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Please CLICK ITEMS, GATE, PORTER, AND MEMBRANE in the picture!
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<!-- Menu -->
 
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<ul id="menu">
 
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<li><a href="http://openwetware.org/wiki/Biomod/2012/Tohoku/Team_Sendai ">Top</a></li>
 
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<li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea ">Project</a></li>
 
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<li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Design">Design</a>
 
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<li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Simulation">Simulation</a>
 
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<a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Experiment ">Experiment</a>
 
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<ul>
 
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<a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/ Experiment#Gate">Gate</a> </li>
 
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<a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Experiment#Porter">Porter</a> </li>
 
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<li> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/ Experiment#Membrane">Membrane</a>
 
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<a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Achievement">Achievement</a>
 
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<a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Diary">Diary</a>
 
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<a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Team ">Team</a>
 
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<a href=" http://openwetware.org/wiki/Biomod/2012/Tohoku/Team Sendai/header"></a>
 
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Current revision


Team Sendai Top

Abstract

All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes.
A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.
Here, we decided to create a novel device with such a function.
We have designed a cylindrical injector/extractor device made of DNA origami (we named this device: CELL-GATE). Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.
We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.

Project Image

Our PROJECT CELL-GATE is divided into three sub-projects:
PORTER: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called Porter. Porters are designed to transfer target DNA strands into (or out from) the membrane.
GATE: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a channel. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.
MEMBRANE: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.

D-Heart Porter Gate Membarane Please CLICK ITEMS, GATE, PORTER, AND MEMBRANE in the picture!

Movie



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