Biomod/2012/Tohoku/Team Sendai
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<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a></b>: Cascade of ssDNA capable of capturing & transporting a target DNA <br> | <b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a></b>: Cascade of ssDNA capable of capturing & transporting a target DNA <br> | ||
<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a></b>: Nanostructural channel made of DNA origami that includes PORTER inside. GATE is inserted in the cell membrane <br> | <b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a></b>: Nanostructural channel made of DNA origami that includes PORTER inside. GATE is inserted in the cell membrane <br> | ||
| - | <b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a></b>: We use liposome as model for cell membrane. | + | <b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a></b>: We use liposome as model for the cell membrane. |
</p> | </p> | ||
<img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px"><br> | <img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px"><br> | ||
Revision as of 11:43, 27 October 2012
Abstract
All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes. A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell. Here, we decided to create a novel device with such a function. We have designed a cylindrical injector/extractor device made of DNA origami (we named this device: CELL-GATE). Inside the cylinder, a cascade of single stranded DNAs (ssDNA) is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones. We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.
Project Image
We divided CELL-GATE into three sub-projects with the following functions: (Please click to see more details)
PORTER: Cascade of ssDNA capable of capturing & transporting a target DNA
GATE: Nanostructural channel made of DNA origami that includes PORTER inside. GATE is inserted in the cell membrane
MEMBRANE: We use liposome as model for the cell membrane.
Movie
