Biomod/2012/Tohoku/Team Sendai
From OpenWetWare
| Line 13: | Line 13: | ||
A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.</br> | A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.</br> | ||
Here, we decided to create a novel device with such a function. </br> | Here, we decided to create a novel device with such a function. </br> | ||
| - | We have designed a <b>cylindrical injector/extractor device made of DNA origami (we named this device: CELL-GATE)</b>. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.</br> | + | We have designed a <b>cylindrical injector/extractor device made of DNA origami (we named this device: CELL-GATE)</b>. Inside the cylinder, a cascade of single stranded DNAs (ssDNA) is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.</br> |
We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.</br> | We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.</br> | ||
</p> | </p> | ||
| Line 20: | Line 20: | ||
<div id="pop"> | <div id="pop"> | ||
<h2><!--Abstract-->Project Image</h2> | <h2><!--Abstract-->Project Image</h2> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
<p> | <p> | ||
| - | <strong> | + | <strong>We divided CELL-GATE into three sub-projects with the following functions:</strong> (Please click to see more details) <br> |
| - | <b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a></b>: | + | <b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a></b>: Cascade of ssDNA capable of capturing & transporting a target DNA <br> |
| - | <b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a></b>: | + | <b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a></b>: Nanostructural channel made of DNA origami that includes PORTER inside. GATE is inserted in the cell membrane <br> |
| - | <b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a></b>: | + | <b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a></b>: We use liposome as model for cell membrane. |
</p> | </p> | ||
| + | <img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px"><br> | ||
</div> | </div> | ||
| Line 45: | Line 39: | ||
<br clear = "left"> | <br clear = "left"> | ||
| - | |||
| - | |||
| - | |||
| - | |||
</div> | </div> | ||
</body> | </body> | ||
</html> | </html> | ||
Revision as of 11:42, 27 October 2012
Abstract
All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes. A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell. Here, we decided to create a novel device with such a function. We have designed a cylindrical injector/extractor device made of DNA origami (we named this device: CELL-GATE). Inside the cylinder, a cascade of single stranded DNAs (ssDNA) is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones. We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.
Project Image
We divided CELL-GATE into three sub-projects with the following functions: (Please click to see more details)
PORTER: Cascade of ssDNA capable of capturing & transporting a target DNA
GATE: Nanostructural channel made of DNA origami that includes PORTER inside. GATE is inserted in the cell membrane
MEMBRANE: We use liposome as model for cell membrane.
Movie
