Biomod/2012/Tohoku/Team Sendai

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A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.</br>
A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.</br>
Here, we decided to create a novel device with such a function. </br>
Here, we decided to create a novel device with such a function. </br>
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We have designed a cylindrical injector/extractor device made of DNA origami. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.</br>
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We have designed a <b>cylindrical injector/extractor device made of DNA origami (we named this device: CELL-GATE)</b>. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.</br>
We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.</br>
We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.</br>
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<img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px"><br>
<img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px"><br>
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<strong>PORTER</strong>: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called  Porter. Once the outer-most Porter binds to a target DNA, the target is passed to the inner ones one by one.</br>
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<strong>Our PROJECT CELL-GATE is divided into three sub-projects:</strong><br>
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<strong>GATE</strong>: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a gate. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.</br>
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<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a></b>: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called  Porter. Once the outer-most Porter binds to a target DNA, the target is passed to the inner ones one by one.</br>
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<strong>MEMBRANE</strong>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.
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<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a></b>: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a gate. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.</br>
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<b><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a></b>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.
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Revision as of 10:38, 27 October 2012

Team Sendai Top

Abstract

All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes.
A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.
Here, we decided to create a novel device with such a function.
We have designed a cylindrical injector/extractor device made of DNA origami (we named this device: CELL-GATE). Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.
We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.

Project Image

D-Heart

Our PROJECT CELL-GATE is divided into three sub-projects:
PORTER: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called Porter. Once the outer-most Porter binds to a target DNA, the target is passed to the inner ones one by one.
GATE: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a gate. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.
MEMBRANE: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.

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