Biomod/2012/Tohoku/Team Sendai
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<img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px"><br> | <img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px"><br> | ||
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| - | <strong>PORTER</strong>: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is | + | <strong>PORTER</strong>: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called Porter. Once the outer-most Porter binds to a target DNA, the target is passed to the inner ones one by one.</br> |
| - | <strong>GATE</strong>: Cylindrical nanostructure connecting the inside and outside of | + | <strong>GATE</strong>: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a gate. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.</br> |
| - | <strong>MEMBRANE</strong>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane | + | <strong>MEMBRANE</strong>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane. |
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Revision as of 09:16, 27 October 2012
Abstract
All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes. A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell. Here, we decided to create a novel device with such a function. We have designed a cylindrical injector/extractor device made of DNA origami. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones. We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.
Project Image
PORTER: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called Porter. Once the outer-most Porter binds to a target DNA, the target is passed to the inner ones one by one. GATE: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a gate. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor. MEMBRANE: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.
Movie
