Biomod/2012/Tohoku/Team Sendai
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| - | <h1 | + | <h1>Abstract</h1> |
| - | Abstract</h1> | + | <p> |
| - | <p | + | All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes.</br> |
| - | All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes. | + | A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.</br> |
| - | A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.<br> | + | Here, we decided to create a novel device with such a function. </br> |
| - | Here, we decided to create a novel device with such a function. <br> | + | We have designed a cylindrical injector/extractor device made of DNA origami. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.</br> |
| - | We have designed a cylindrical injector/extractor device made of DNA origami. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.<br> | + | We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.</br> |
| - | We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.< | + | |
| - | + | ||
</p> | </p> | ||
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<h2><!--Abstract-->Project Image</h2> | <h2><!--Abstract-->Project Image</h2> | ||
| - | <img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width=" | + | <img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px"><br> |
| + | <p> | ||
<strong>Porter</strong>: Single stranded DNA. Once the outer-most ssDNA binds to a target, the target is passed to the inner-ones one by one.</br> | <strong>Porter</strong>: Single stranded DNA. Once the outer-most ssDNA binds to a target, the target is passed to the inner-ones one by one.</br> | ||
<strong>Gate</strong>: Cylindrical structure connecting the inside and outside like gates. A cascade of the "Selector" is planted in this. We can use this as an injector or extractor.</br> | <strong>Gate</strong>: Cylindrical structure connecting the inside and outside like gates. A cascade of the "Selector" is planted in this. We can use this as an injector or extractor.</br> | ||
<strong>Membrane</strong>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane. | <strong>Membrane</strong>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane. | ||
| + | </p> | ||
</div> | </div> | ||
Revision as of 03:50, 27 October 2012
Tohoku University
Abstract
All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes. A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell. Here, we decided to create a novel device with such a function. We have designed a cylindrical injector/extractor device made of DNA origami. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones. We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.
Project Image
Porter: Single stranded DNA. Once the outer-most ssDNA binds to a target, the target is passed to the inner-ones one by one. Gate: Cylindrical structure connecting the inside and outside like gates. A cascade of the "Selector" is planted in this. We can use this as an injector or extractor. Membrane: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.
Movie
| Coming soon... |
