Biomod/2012/Tohoku/Team Sendai
From OpenWetWare
(Difference between revisions)
| Line 14: | Line 14: | ||
<div id="Container"> | <div id="Container"> | ||
<!—二段組み本文 --> | <!—二段組み本文 --> | ||
| - | < | + | |
| - | <p> | + | <h1 style="width:900px;clear:left;margin:15px;"> |
| - | + | Abstract</h1> | |
| - | + | <p style="width:900px;clear:left;margin:15px;"> | |
| - | + | All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes. | |
| - | + | A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.<br> | |
| - | + | Here, we decided to create a novel device with such a function. <br> | |
| - | + | We have designed a cylindrical injector/extractor device made of DNA origami. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.<br> | |
| + | We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.<br> | ||
| + | <br> | ||
</p> | </p> | ||
| Line 28: | Line 30: | ||
<h2><!--Abstract-->Project Image</h2> | <h2><!--Abstract-->Project Image</h2> | ||
<img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="400px"><br> | <img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="400px"><br> | ||
| + | <strong>Porter</strong>: Single stranded DNA. Once the outer-most ssDNA binds to a target, the target is passed to the inner-ones one by one.</br> | ||
| + | <strong>Gate</strong>: Cylindrical structure connecting the inside and outside like gates. A cascade of the "Selector" is planted in this. We can use this as an injector or extractor.</br> | ||
| + | <strong>Membrane</strong>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane. | ||
</div> | </div> | ||
| Line 45: | Line 50: | ||
</p> | </p> | ||
</div> | </div> | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<a href=" http://openwetware.org/wiki/Biomod/2012/Team_Sendai_Old">Test page</a> | <a href=" http://openwetware.org/wiki/Biomod/2012/Team_Sendai_Old">Test page</a> | ||
Revision as of 00:03, 27 October 2012
Tohoku University
Abstract
All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes.
A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.
Here, we decided to create a novel device with such a function.
We have designed a cylindrical injector/extractor device made of DNA origami. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.
We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.
Project Image
Porter: Single stranded DNA. Once the outer-most ssDNA binds to a target, the target is passed to the inner-ones one by one. Gate: Cylindrical structure connecting the inside and outside like gates. A cascade of the "Selector" is planted in this. We can use this as an injector or extractor. Membrane: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.
Movie
| Coming soon... |
