Biomod/2012/Titech/Nano-Jugglers/Results/EDAC conjugation: Difference between revisions

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|[[Image:BIOMOD-TNJ-CrAu_3_2s-13.jpg|200px]]
|[[Image:BIOMOD-TNJ-CrAu_3_2s-13.jpg|200px]]
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|Fluorescence
|'''Fluorescence'''
|'''observed'''
|'''observed'''
|'''Not observed'''
|'''Not observed'''

Revision as of 05:49, 2 September 2012


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} </style> </head> <BODY> <div id="biomodlink"> <<a href="http://openwetware.org/wiki/Biomod">BIOMOD</a>|<a href="http://openwetware.org/wiki/Biomod/2012">2012</a>|Titech Nano-Jugglers </div> <div id="header"> <div id="navigation"> <div id="menu"> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers"><br>Home<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Team/Students"><br>Team<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Project"><br>Project<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Results">Results<br>&<br>Methods</a></font></li> <li class="ach"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Achievements"><br>Achievements<br><br></a> <li class="sup"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Protocols"><br>Suppl. Info.<br><br></a></li> <li class="none"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Acknowledgement"><br>Acknowledgements<br><br></a></li> </ul> </div> </div> </div> </BODY> </html>


Amino Modified DNA And Polystyrene Conjugation

After the protocol, to confirm that amino modified DNA is bind to polystyrene ,we hybridize the DNA with FAM and observe them under blue light by microscope.(Control experiment)

RESULT

Added Reagent Polystyren beads
DNA which bind with beads
Complementary FAM 		Polystyren beads
DNA which bind with beads

 Polystyren beads

Complementary FAM
Under Transmitted Light
Under Blue Light
Fluorescence observed Not observed Not observed
※We use polystyrene beads whose 1/4 was covered with Gold and 1/2 was covered with chromium.

From this result, we concluded that amino modified DNA is bind to polystyrene

Observation Conditions

ISO6400
Exposure time（Transmitted Light）１／１００seconds
Exposure time（Blue Light）２seconds
Magnification １０×４０＝４００
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