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Amino modified DNA and polystyrene conjugation by EDAC

After the protocol, to confirm that amino modified DNA is bind to polystyrene ,we hybridize the DNA with FAM and observe them under blue light by microscope.(Control experiment)

RESULT

Added Reagent	Polystyren beads DNA which bind with beads Complementary FAM	Polystyren beads DNA which bind with beads	Polystyren beads Complementary FAM
Under Transmitted Light
Under Blue Light
Fluorescence	observed	Not observed	Not observed

※We use polystyrene beads whose 1/4 was covered with Gold and 1/2 was covered with chromium.

From this result, we concluded that amino modified DNA is bind to polystyrene

Observation Conditions

ISO6400

Exposure time（Transmitted Light）１／１００seconds

Exposure time（Blue Light）２seconds

Magnification １０×４０＝４００