Biomod/2012/Titech/Nano-Jugglers/Results/EDAC conjugation
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:※We use polystyrene beads whose 1/4 was covered with Gold and 1/2 was covered with chromium.<br><br> | :※We use polystyrene beads whose 1/4 was covered with Gold and 1/2 was covered with chromium.<br><br> | ||
Revision as of 03:04, 4 October 2012
Amino modified DNA and polystyrene conjugation by EDAC
- After the protocol, to confirm that amino modified DNA is bind to polystyrene ,we hybridize the DNA with FAM and observe them under blue light by microscope.(Control experiment)
RESULT
Added Reagent Polystyren beads
DNA which bind with beads
Complementary FAMPolystyren beads
DNA which bind with beads
Polystyren beads
Complementary FAMUnder Transmitted Light 
Under Blue Light 
Fluorescence observed Not observed Not observed
- ※We use polystyrene beads whose 1/4 was covered with Gold and 1/2 was covered with chromium.
- From this result, we concluded that amino modified DNA is bind to polystyrene
Observation Conditions
- ISO6400
- Exposure time（Transmitted Light）１／１００seconds
- Exposure time（Blue Light）２seconds
- Magnification １０×４０＝４００
