Biomod/2012/Titech/Nano-Jugglers/Results

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} </style> </head> <BODY> <div id="biomodlink"> <<a href="http://openwetware.org/wiki/Biomod">BIOMOD</a>|<a href="http://openwetware.org/wiki/Biomod/2012">2012</a>|Titech Nano-Jugglers </div> <div id="header"> <div id="navigation"> <div id="menu"> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers"><br>Home<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Team/Students"><br>Team<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Project"><br>Project<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Results">Results<br>&<br>Methods</a></font></li> <li class="ach"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Achievements"><br>Achievements<br><br></a> <li class="sup"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Protocols"><br>Suppl. Info.<br><br></a></li> <li class="none"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Acknowledgement"><br>Acknowledgements<br><br></a></li> </ul> </div> </div> </div> </BODY> </html>

    We have set 3 goals, that is "Energy production for rail-free movement", "Speeding up", and "Direction control".

Experimental results

<html><A href=#TOP title="Vapor deposition"><IMG width="300px" src="http://openwetware.org/images/a/a5/Vapor_deposition.jpg"></A></html> <html><A href=#TOP title="SAM conjugation"><IMG width="300px" src="http://openwetware.org/images/4/48/Thiol_.jpg"></A></html> <html><A href=#TOP title="EDAC conjugation"><IMG width="300px" src="http://openwetware.org/images/3/3d/EDAC_conjugation.jpg"></A></html>
<html><A href=#TOP title="DNA design"><IMG width="300px" src="http://openwetware.org/images/b/b2/DNA_design.jpg"></A></html> <html><A href=#TOP title="半白金　1μの動画"><IMG width="300px" src="http://openwetware.org/images/8/80/Damy.png"></A></html> <html><A href=#TOP title=DNA hybrudization><IMG width="300px"

src="http://openwetware.org/images/f/f1/DNA_hybrudization.jpg"></A></html>

<html><A href=#TOP title="ハイスピードカメラ"><IMG width="300px" src="http://openwetware.org/images/8/80/Damy.png"></A></html> <html><A href=#TOP><IMG width="300px" src="http://openwetware.org/images/e/ed/%E3%82%AB%E3%82%BF%E3%83%A9%E3%83%BC%E3%82%BC.jpg"></A></html> <html><A href=#TOP title="Azobenzene"><IMG width="300px" src="http://openwetware.org/images/e/ea/Azobenzene.jpg"></A></html>

Vapor deposition of Au and Cr on the polystyrene body

>>see Method
File:Body construction.png
  • Figure1
This picture shows the observation results of the 40 micron polystyrene beads.


  • Figure2
This picture shows the observation results of 40 micron polystyrene beads with completely covered by gold.


  • Figure3
This picture shows the observation results of the 40 micron silica beads with half vapor deposition of gold.


  • Figure4
This picture shows the observation result of 40 micron polystyrene beads with half vapor deposition of gold.


  • Discussion
From these pictures, we observed that PVD can make hemisphere membrane of gold. By comparing the color of figure1 and figure2, micro-beads completely covered by gold are more black and have a metallic luster.　 From figure3 , it is possible that the size of 40μm beads can deposit gold as hemisphere in that the colors of beads sphere’s limbs are different .Figure 4 shows that 10 micron sized beads can also deposit gold as hemisphere, because metallic luster color and white color are dotted in one sphere.
Figure1 40 micron polystyrene beads
Figure2 40 micron polystyrene beads with completely covered by gold
Figure3 40 micron silica beads with half vapor deposition of gold
Figure4 40 micron polystyrene beads with half vapor deposition of gold

DNA conjugation

File:Body construction.png
SAM

    We bond Au with DNA using thiol.

>>see more Method

EDAC     We bond polystyrene-beads with DNA using EDAC.

>>see more Method

DNA Design

    We used NUPACK and designed five strands of DNA.

>>see more Method

Observation of platinum hemisphere

DNA hybridization in solution of H2O2

    We used PAGE electrophoresis to ascertain the stability of DNA duplex in thin H₂O₂ solution 1%~5%.

>>see more Method

Analysis of platinum by High-speed camera

Energy production by using catalase

Dissociation of azobenzene-modified DNA by UV-light irradiation

    Azobenzene including DNA can easily dissociate its duplex by irradiating UV-light. We put this switching system in JET body and enabled its control.

>>see more Method
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