Biomod/2012/Titech/Nano-Jugglers/Results - Revision history

Kensuke Hoshi at 07:04, 28 October 2012

2012-10-28T07:04:23Z

←Older revision		Revision as of 07:04, 28 October 2012
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	:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Simulation\|simulation models]]		:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Simulation\|simulation models]]
	:    The simulation also demonstrated that the Biomolecular Rocket with many platinum catalysts on the body can move twice faster than a microbead with a hemispherical platinum surface moves because of the difference in the catalytic surface areas between them (Fig. 2.2).		:    The simulation also demonstrated that the Biomolecular Rocket with many platinum catalysts on the body can move twice faster than a microbead with a hemispherical platinum surface moves because of the difference in the catalytic surface areas between them (Fig. 2.2).
-	:    Figure 2.2 shows instantaneous speed at each time steps of kinesin motor, 10 μm beads with a hemispherical platinum surface (catalytic motor without DNA), and 10 μm of the Biomolecular Rocket. We take the average of 100 time step's instantaneous speed of each molecular ~~motor　and~~ make a comparison among them.	+	:    Figure 2.2 shows instantaneous speed at each time steps of kinesin motor, 10 μm beads with a hemispherical platinum surface (catalytic motor without DNA), and 10 μm of the Biomolecular Rocket. We take the average of 100 time step's instantaneous speed of each molecular motor and make a comparison among them.
	:    As a result, the average speed of the Biomolecular Rocket is calculated as 11.2 μm/s, and the average speed of catalytic motor without DNA is 6.8 μm/s. We assumed that Kinesin moves straightforward at 1.0 μm/s all the time, Kinesin's average speed is calculated as 1.0 μm/s.		:    As a result, the average speed of the Biomolecular Rocket is calculated as 11.2 μm/s, and the average speed of catalytic motor without DNA is 6.8 μm/s. We assumed that Kinesin moves straightforward at 1.0 μm/s all the time, Kinesin's average speed is calculated as 1.0 μm/s.
	:    Therefore, we estimate that the Biomolecular Rocket moves faster than kinesin. Moreover, the Biomolecular Rocket can get more speed by surface enlargement of catalyst with DNA.		:    Therefore, we estimate that the Biomolecular Rocket moves faster than kinesin. Moreover, the Biomolecular Rocket can get more speed by surface enlargement of catalyst with DNA.

Kensuke Hoshi: /* 0.1. Selective coating of the body */

2012-10-28T06:57:03Z

0.1. Selective coating of the body

←Older revision		Revision as of 06:57, 28 October 2012
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	:    Figure 0.1a, b and c are microscope images of 40 μm microbeads. Figure 0.1a shows microbeads before vapor deposition of metals. Figure 0.1b shows microbeads after vapor deposition of Au (gold) on the microbeads. Figure 0.1c shows microbeads after additional vapor deposition of Cr (chromium) on the Au-deposited microbeads. The microbeads had three types of surface areas because the angular alignment of the beads was changed when Cr was deposited on the Au-deposited microbeads.		:    Figure 0.1a, b and c are microscope images of 40 μm microbeads. Figure 0.1a shows microbeads before vapor deposition of metals. Figure 0.1b shows microbeads after vapor deposition of Au (gold) on the microbeads. Figure 0.1c shows microbeads after additional vapor deposition of Cr (chromium) on the Au-deposited microbeads. The microbeads had three types of surface areas because the angular alignment of the beads was changed when Cr was deposited on the Au-deposited microbeads.
-	:    Similarly, Figure 0.1e, f and g are microscope images of 10 μm microbeads. Figure 0.1e shows microbeads before vapor deposition of metals. Figure 0.1f shows microbeads after vapor deposition of Au on the microbeads. Figure 0.1g shows microbeads after additional vapor deposition of Cr on the Au-deposited microbeads. The 10 μm microbeads probably had three types of surface areas. From these results, we conclude that selective coating of microbeads for Biomolecular Rocket was achieved.	+	:    Similarly, Figure 0.1e, f and g are microscope images of 10 μm microbeads. Figure 0.1e shows microbeads before vapor deposition of metals. Figure 0.1f shows microbeads after vapor deposition of Au on the microbeads. Figure 0.1g shows microbeads after additional vapor deposition of Cr on the Au-deposited microbeads. The 10 μm microbeads probably had three types of surface areas. From these results, we conclude that selective coating of microbeads for the Biomolecular Rocket was achieved.
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	\|  <html><body><nobr>40 μm silica beads</nobr></body></html>		\|  <html><body><nobr>40 μm silica beads</nobr></body></html>

Kensuke Hoshi: /* 2.1. Power supply for the high-speed movement with platinum catalytic engines */

2012-10-28T06:19:52Z

2.1. Power supply for the high-speed movement with platinum catalytic engines

←Older revision		Revision as of 06:19, 28 October 2012
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	:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/High-speed_camera\|methods and full length movie]]<br><br>		:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/High-speed_camera\|methods and full length movie]]<br><br>
	:    Movie 2.1.1a shows the movement of 0.15-0.40 μm platinum particles in 3% H<sub>2</sub>O<sub>2</sub> solution. Movie 2.1.1b is the movement of platinum particles observed by a high-speed camera (the upper left of the movie) and its analyzed results (the right of the movie). The analyzed results show the values of acceleration (top), velocity (the second from the top), coordinate x (third), and coordinate y (bottom) of platinum movement. From the results, we found the relationships between the growth of bubble radius and the speed of platinum. Using the relationships and the kinetic parameters, we did numerical simulations of the movement of the Biomolecular Rocket (see Section 2.2).		:    Movie 2.1.1a shows the movement of 0.15-0.40 μm platinum particles in 3% H<sub>2</sub>O<sub>2</sub> solution. Movie 2.1.1b is the movement of platinum particles observed by a high-speed camera (the upper left of the movie) and its analyzed results (the right of the movie). The analyzed results show the values of acceleration (top), velocity (the second from the top), coordinate x (third), and coordinate y (bottom) of platinum movement. From the results, we found the relationships between the growth of bubble radius and the speed of platinum. Using the relationships and the kinetic parameters, we did numerical simulations of the movement of the Biomolecular Rocket (see Section 2.2).
-	:    Figure. 2.1.2 shows the graph of the mean square displacement of the platinum particle. We can calculate mean speed of platinum particle by making a quadratic curve fitting and calculating square root of coefficient of the curve. We calculated that the value of quadratic coefficient was 115.5 mm<sup>2</sup>/s<sup>2</sup>, and the average speed was calculated as 10.7 mm/s. Platinum particles in H<sub>2</sub>O<sub>2</sub> solution can moved at about '''''10,000 time’s faster speed than kinesin.'''''	+	:    Figure. 2.1.2 shows the graph of the mean square displacement of the platinum particle. We can calculate mean speed of platinum particle by making a quadratic curve fitting and calculating square root of coefficient of the curve. We calculated that the value of quadratic coefficient was 115.5 mm<sup>2</sup>/s<sup>2</sup>, and the average speed was calculated as 10.7 mm/s. Assuming that kinesin moves at 1μm/s, Platinum particles in H<sub>2</sub>O<sub>2</sub> solution can moved at about '''''10,000 time’s faster speed than kinesin.'''''

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Kensuke Hoshi: /* 2.1. Power supply for the high-speed movement with platinum catalytic engines */

2012-10-28T06:17:52Z

2.1. Power supply for the high-speed movement with platinum catalytic engines

←Older revision		Revision as of 06:17, 28 October 2012
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	:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/High-speed_camera\|methods and full length movie]]<br><br>		:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/High-speed_camera\|methods and full length movie]]<br><br>
	:    Movie 2.1.1a shows the movement of 0.15-0.40 μm platinum particles in 3% H<sub>2</sub>O<sub>2</sub> solution. Movie 2.1.1b is the movement of platinum particles observed by a high-speed camera (the upper left of the movie) and its analyzed results (the right of the movie). The analyzed results show the values of acceleration (top), velocity (the second from the top), coordinate x (third), and coordinate y (bottom) of platinum movement. From the results, we found the relationships between the growth of bubble radius and the speed of platinum. Using the relationships and the kinetic parameters, we did numerical simulations of the movement of the Biomolecular Rocket (see Section 2.2).		:    Movie 2.1.1a shows the movement of 0.15-0.40 μm platinum particles in 3% H<sub>2</sub>O<sub>2</sub> solution. Movie 2.1.1b is the movement of platinum particles observed by a high-speed camera (the upper left of the movie) and its analyzed results (the right of the movie). The analyzed results show the values of acceleration (top), velocity (the second from the top), coordinate x (third), and coordinate y (bottom) of platinum movement. From the results, we found the relationships between the growth of bubble radius and the speed of platinum. Using the relationships and the kinetic parameters, we did numerical simulations of the movement of the Biomolecular Rocket (see Section 2.2).
-	:    Figure. 2.1.2 shows the graph of the mean square displacement of the platinum particle~~. We assumed diffusion coefficient of this platinum particle and took away displacement by Brownian movement from total mean square displacement~~. We can calculate mean ~~square~~ speed of platinum particle by making a quadratic curve fitting and calculating square root of coefficient of the curve. We calculated that the value of quadratic coefficient was 77.2 mm<sup>2</sup>/s<sup>2</sup>, and the average speed was calculated as 8.8 mm/s. Platinum particles moved at about '''''8,~~800~~ time’s faster speed than kinesin.'''''	+	:    Figure. 2.1.2 shows the graph of the mean square displacement of the platinum particle. We can calculate mean speed of platinum particle by making a quadratic curve fitting and calculating square root of coefficient of the curve. We calculated that the value of quadratic coefficient was 115.5 mm<sup>2</sup>/s<sup>2</sup>, and the average speed was calculated as 10.7 mm/s. Platinum particles in H<sub>2</sub>O<sub>2</sub> solution can moved at about '''''10,000 time’s faster speed than kinesin.'''''

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Kensuke Hoshi: /* 3.2. Achievement of the photo-switchable DNA system */

2012-10-28T04:41:33Z

3.2. Achievement of the photo-switchable DNA system

←Older revision		Revision as of 04:41, 28 October 2012
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	<html><body><font size="5">We have succeeded in development of a photo-switchable DNA system.</font></body></html><br>		<html><body><font size="5">We have succeeded in development of a photo-switchable DNA system.</font></body></html><br>
	>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/Dissociation_of_photoresponsive_DNA\|methods]]<br><br>		>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/Dissociation_of_photoresponsive_DNA\|methods]]<br><br>
-	We ~~introduce~~ the photo-switching DNA system into Biomolecular Rocket in order to control its moving direction.	+	We introduced the photo-switching DNA system into Biomolecular Rocket in order to control its moving direction.
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	:   To investigate the relationship between the strength of UV light and the irradiation time for dissociation of the photoresponsive DNA and determine the valid time for UV light irradiation, we examined two types of strength of UV light.		:   To investigate the relationship between the strength of UV light and the irradiation time for dissociation of the photoresponsive DNA and determine the valid time for UV light irradiation, we examined two types of strength of UV light.

Kensuke Hoshi: /* 3. Introduction of a photo-switchable DNA system for the directional control */

2012-10-28T04:36:42Z

3. Introduction of a photo-switchable DNA system for the directional control

←Older revision		Revision as of 04:36, 28 October 2012
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	<li>Design of a photo-switchable DNA system for the directional control		<li>Design of a photo-switchable DNA system for the directional control
-	<li>Investigation of the dissociation rate of the photo-~~switchahble~~ DNA duplex by UV light irradiation experiments	+	<li>Investigation of the dissociation rate of the photo-switchable DNA duplex by UV light irradiation experiments
	<li>Investigation of the directional control of Biomolecular Rocket with the photo-switchable DNA system by numerical simulations		<li>Investigation of the directional control of Biomolecular Rocket with the photo-switchable DNA system by numerical simulations
	</ol></body></html>		</ol></body></html>

Kensuke Hoshi: /* 2.2. Numerical estimation of the speed of Biomolecular Rocket */

2012-10-28T04:33:36Z

2.2. Numerical estimation of the speed of Biomolecular Rocket

←Older revision		Revision as of 04:33, 28 October 2012
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-	==2.2. Numerical estimation of the speed of Biomolecular Rocket==	+	==2.2. Numerical estimation of the speed of the Biomolecular Rocket==
-	:<html><body><font size="5">The numerical simulation revealed that Biomolecular Rocket can move ten times faster than kinesin.</font></body></html><br>	+	:<html><body><font size="5">The numerical simulation revealed that the Biomolecular Rocket can move ten times faster than kinesin.</font></body></html><br>
	:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Simulation\|simulation models]]		:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Simulation\|simulation models]]
-	:    The simulation also demonstrated that Biomolecular Rocket with many platinum catalysts on the body can move twice faster than a microbead with a hemispherical platinum surface moves because of the difference in the catalytic surface areas between them (Fig. 2.2).	+	:    The simulation also demonstrated that the Biomolecular Rocket with many platinum catalysts on the body can move twice faster than a microbead with a hemispherical platinum surface moves because of the difference in the catalytic surface areas between them (Fig. 2.2).
-	:    Figure 2.2 shows instantaneous speed at each time steps of kinesin motor, 10 μm beads with a hemispherical platinum surface (catalytic motor without DNA), and 10 μm of Biomolecular Rocket. We take the average of 100 time step's instantaneous speed of each molecular motor　and make a comparison among them.	+	:    Figure 2.2 shows instantaneous speed at each time steps of kinesin motor, 10 μm beads with a hemispherical platinum surface (catalytic motor without DNA), and 10 μm of the Biomolecular Rocket. We take the average of 100 time step's instantaneous speed of each molecular motor　and make a comparison among them.
-	:    As a result, the average speed of Biomolecular Rocket is calculated as 11.2 μm/s, and the average speed of catalytic motor without DNA is 6.8 μm/s. We assumed that Kinesin moves straightforward at 1.0 μm/s all the time, Kinesin's average speed is calculated as 1.0 μm/s.	+	:    As a result, the average speed of the Biomolecular Rocket is calculated as 11.2 μm/s, and the average speed of catalytic motor without DNA is 6.8 μm/s. We assumed that Kinesin moves straightforward at 1.0 μm/s all the time, Kinesin's average speed is calculated as 1.0 μm/s.
-	:    Therefore, we estimate that ~~biomolecular rocket~~ moves faster than kinesin. Moreover, Biomolecular ~~rocket~~ can get more speed by surface enlargement of catalyst with DNA.	+	:    Therefore, we estimate that the Biomolecular Rocket moves faster than kinesin. Moreover, the Biomolecular Rocket can get more speed by surface enlargement of catalyst with DNA.
	:{\|style="margin-left:100px" border="1"		:{\|style="margin-left:100px" border="1"
	\|[[Image:Hi_speed.png\|Simulation of high-speed\|700px]]		\|[[Image:Hi_speed.png\|Simulation of high-speed\|700px]]

Kensuke Hoshi: /* 3.3. Directional control of Biomolecular Rocket by the photo-switchable DNA system */

2012-10-28T04:33:19Z

3.3. Directional control of Biomolecular Rocket by the photo-switchable DNA system

←Older revision		Revision as of 04:33, 28 October 2012
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-	==3.3. Directional control of Biomolecular Rocket by the photo-switchable DNA system==	+	==3.3. Directional control of the Biomolecular Rocket by the photo-switchable DNA system==
-	:    <html><body><font size="5">By numerical simulations, we found that Biomolecular Rocket changes its direction by the UV light irradiation even though Biomolecular Rocket is affected by the viscous resistance force and Brownian dynamics of water.</font></body></html><br>	+	:    <html><body><font size="5">By numerical simulations, we found that the Biomolecular Rocket changes its direction by the UV light irradiation even though the Biomolecular Rocket is affected by the viscous resistance force and Brownian dynamics of water.</font></body></html><br>
	:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Simulation\|Simulation Models]]		:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Simulation\|Simulation Models]]
-	:    Figure 3.3 shows the trajectories of Biomolecular Rockets of 10 μm in diameter by the numerical simulation. Each trajectory shows the movement of Biomolecular Rocket that detaches catalytic engines after the irradiation of UV light when 10 seconds, 15 seconds, 20 seconds, and 25 seconds passed. This simulation results suggested that Biomolecular Rocket can change its direction immediately after all catalytic engines are dissociated even though the viscous resistance and Brownian movement prevented the controlled movement of Biomolecular Rocket. From these results, we conclude that Biomolecular Rocket changes its direction by UV light irradiation.	+	:    Figure 3.3 shows the trajectories of the the Biomolecular Rockets of 10 μm in diameter by the numerical simulation. Each trajectory shows the movement of the Biomolecular Rocket that detaches catalytic engines after the irradiation of UV light when 10 seconds, 15 seconds, 20 seconds, and 25 seconds passed. This simulation results suggested that the Biomolecular Rocket can change its direction immediately after all catalytic engines are dissociated even though the viscous resistance and Brownian movement prevented the controlled movement of the Biomolecular Rocket. From these results, we conclude that the Biomolecular Rocket changes its direction by UV light irradiation.
	{\|style="margin-left:170px" width="600px" border="1"		{\|style="margin-left:170px" width="600px" border="1"
	\|[[Image:ControlResult画像.png\|Control simulation\|600px]]		\|[[Image:ControlResult画像.png\|Control simulation\|600px]]

Kensuke Hoshi: /* 2.2. Numerical estimation of the speed of Biomolecular Rocket */

2012-10-28T04:30:41Z

2.2. Numerical estimation of the speed of Biomolecular Rocket

←Older revision		Revision as of 04:30, 28 October 2012
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	==2.2. Numerical estimation of the speed of Biomolecular Rocket==		==2.2. Numerical estimation of the speed of Biomolecular Rocket==
-	:<html><body><font size="5">The numerical simulation revealed that Biomolecular Rocket can move at ten times faster than kinesin ~~moves~~.</font></body></html><br>	+	:<html><body><font size="5">The numerical simulation revealed that Biomolecular Rocket can move ten times faster than kinesin.</font></body></html><br>
	:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Simulation\|simulation models]]		:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Simulation\|simulation models]]
	:    The simulation also demonstrated that Biomolecular Rocket with many platinum catalysts on the body can move twice faster than a microbead with a hemispherical platinum surface moves because of the difference in the catalytic surface areas between them (Fig. 2.2).		:    The simulation also demonstrated that Biomolecular Rocket with many platinum catalysts on the body can move twice faster than a microbead with a hemispherical platinum surface moves because of the difference in the catalytic surface areas between them (Fig. 2.2).

Kensuke Hoshi: /* 2.1. Power supply for the high-speed movement with platinum catalytic engines */

2012-10-28T04:27:19Z

2.1. Power supply for the high-speed movement with platinum catalytic engines

←Older revision		Revision as of 04:27, 28 October 2012
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	\|style="padding:10px" colspan="2"\|Movie. 2.1.1    Analyses of the speed of platinum in solution of H<sub>2</sub>O<sub>2</sub>.<br>		\|style="padding:10px" colspan="2"\|Movie. 2.1.1    Analyses of the speed of platinum in solution of H<sub>2</sub>O<sub>2</sub>.<br>
-	(a)  Platinum movement in solution of H<sub>2</sub>O<sub>2</sub>          (b)  ~~Analises~~ of the speed of plutinum by High-speed camera	+	(a)  Platinum movement in solution of H<sub>2</sub>O<sub>2</sub>          (b)  Analyses of the speed of plutinum by High-speed camera
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