Biomod/2012/Titech/Nano-Jugglers/Results: Difference between revisions

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{{Titech/Nano-Jugglers/HEAD}}
{{Titech/Nano-Jugglers/HEAD}}
__NOTOC__


:__TOC__
:__TOC__
{|style="margin-left:10px"
|-
|[[Image:Goals.jpg|400px]]
|[[Image:Goals.png|500px]]
    We have set 3 goals, that is "Energy production for rail-free movement", "Speeding up", and "Direction control".
|}
=Methods=
:{|
|-
|width = "300px"|<HTML><A href=#TOP title="Vapor deposition"><IMG width="300px" src="http://openwetware.org/images/a/a5/Vapor_deposition.jpg"></A></HTML>
|width = "300px"|<HTML><A href=#TOP title="SAM conjugation"><IMG width="300px" src="http://openwetware.org/images/4/48/Thiol_.jpg"></A></HTML>
|width = "300px"|<HTML><A href=#TOP title="EDAC conjugation"><IMG width="300px" src="http://openwetware.org/images/3/3d/EDAC_conjugation.jpg"></A></HTML>
|-
|width = "300px"|<HTML><A href=#TOP title="DNA design"><IMG width="300px" src="http://openwetware.org/images/b/b2/DNA_design.jpg"></A></HTML>
|width = "300px"|<HTML><A href=#TOP title="半白金　1μの動画"><IMG width="300px" src="http://openwetware.org/images/8/80/Damy.png"></A></HTML>
|width = "300px"|<HTML><A href=#TOP title=DNA hybrudization><IMG width="300px"
src="http://openwetware.org/images/f/f1/DNA_hybrudization.jpg"></A></HTML>
|-
|width = "300px"|<HTML><A href=#TOP title="ハイスピードカメラ"><IMG width="300px" src="http://openwetware.org/images/8/80/Damy.png"></A></HTML>
|width = "300px"|<HTML><A href=#TOP><IMG width="300px" src="http://openwetware.org/images/e/ed/%E3%82%AB%E3%82%BF%E3%83%A9%E3%83%BC%E3%82%BC.jpg"></A></HTML>
|width = "300px"|<HTML><A href=#TOP title="Azobenzene"><IMG width="300px" src="http://openwetware.org/images/e/ea/Azobenzene.jpg"></A></HTML>
|}
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>
==Vapor deposition of Au and Cr on the polystyrene body==
==Vapor deposition of Au and Cr on the polystyrene body==
:{|
|-
|-
|width="300px"|[[Image:Body　construction.png|300px]]
|-
|width="300px"|[[Image:Vapor_deposition.jpg|300px]]
|&nbsp;&nbsp;&nbsp;&nbsp;Deposition is a method for forming a metal thin film on the surface of the substrate.In this project, Gold and Chromium were deposited on the polystyrene surface in order to conjugate DNA onto the specific location on the surface.
:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/Vapor_Deposition|Method]]
|}
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>
==DNA conjugation==
:{|
|-
|width="300px"|[[Image:Body　construction.png|300px]]
|-
|width="300px"|[[Image:Thiol_.jpg|center|300px]]
|'''SAM'''
&nbsp;&nbsp;&nbsp;&nbsp;We bond Au with DNA using thiol.
:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/Thiol_conjugation|Method]]
|-
|}


:{|
|-
|width="300px"|[[Image:EDAC_conjugation.jpg|center|300px]]
|
'''EDAC'''
&nbsp;&nbsp;&nbsp;&nbsp;We bond polystyrene-beads with DNA using EDAC.
:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/EDAC_conjugation|Method]]
|}


<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>
==DNA Design==
==DNA Design==
:{|
|-
|width="300px"|[[Image:Body and Control.png|center|300px]]
|-
|width="300px"|[[Image:DNA_design.jpg|center|300px]]
|
&nbsp;&nbsp;&nbsp;&nbsp;We used NUPACK and designed five strands of DNA.<br>
:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/DNA_Design|Method]]
|}
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>


==Observation of platinum hemisphere==
:{|
|-
|width="300px"|[[Image:Rail-free and High-speed.png|center|300px]]
|-
|width="300px"|[[Image:Damy.png|center|300px]]
|
|}
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>
==DNA Conjugation==


==DNA hybridization in solution of H<small>2</small>O<small>2</small>==
:{|
|-
|width="300px"|[[Image:Body and Rail-free and High-speed.png|center|300px]]
|-
|width="300px"|[[Image:BIOMODTNJEPR TITLE.JPG|center|300px]]
|
&nbsp;&nbsp;&nbsp;&nbsp;We used PAGE electrophoresis to ascertain the stability of DNA duplex in thin H₂O₂ solution 1%~5%.
:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/DNA_hybridization|Method]]
|}
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>
半カタラーゼ半白金の進行計画


==Analysis of platinum by High-speed camera==
:{|
|-
|width="300px"|[[Image:Rail-free and High-speed.png|center|300px]]
|-
|width="300px"|[[Image:Damy.png|center|300px]]
|


|}
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>
==DNA hybridization in solution of H2O2==


==Energy production by using catalase==
:{|
|-
|width="300px"|[[Image:High-speed.png|center|300px]]
|-
|width="300px"|[[Image:Damy.png|center|300px]]
|
|}
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>
速度計算及びハイスピードカメラの解析


 
==Dissociation of azobenzene-modified DNA by UV-light irradiation==
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>
:{|[[
シミュレーション
|-
|width="300px"|[[Image:Directional control.png|300px|center]]
|-
|width="300px"|[[Image:Azobenzene.jpg|300px|center]]
|
&nbsp;&nbsp;&nbsp;&nbsp;Azobenzene including DNA can easily dissociate its duplex by irradiating UV-light. We put this switching system in JET body and enabled its control.
:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/Azobenzene_Tweezer|Method]]
|}
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>

Revision as of 09:13, 30 September 2012


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} </style> </head> <BODY> <div id="biomodlink"> <<a href="http://openwetware.org/wiki/Biomod">BIOMOD</a>|<a href="http://openwetware.org/wiki/Biomod/2012">2012</a>|Titech Nano-Jugglers </div> <div id="header"> <div id="navigation"> <div id="menu"> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers"><br>Home<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Team/Students"><br>Team<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Project"><br>Project<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Results">Results<br>&<br>Methods</a></font></li> <li class="ach"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Achievements"><br>Achievements<br><br></a> <li class="sup"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Protocols"><br>Suppl. Info.<br><br></a></li> <li class="none"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Acknowledgement"><br>Acknowledgements<br><br></a></li> </ul> </div> </div> </div> </BODY> </html>

    We have set 3 goals, that is "Energy production for rail-free movement", "Speeding up", and "Direction control".

Methods

<html><A href=#TOP title="Vapor deposition"><IMG width="300px" src="http://openwetware.org/images/a/a5/Vapor_deposition.jpg"></A></html> <html><A href=#TOP title="SAM conjugation"><IMG width="300px" src="http://openwetware.org/images/4/48/Thiol_.jpg"></A></html> <html><A href=#TOP title="EDAC conjugation"><IMG width="300px" src="http://openwetware.org/images/3/3d/EDAC_conjugation.jpg"></A></html>
<html><A href=#TOP title="DNA design"><IMG width="300px" src="http://openwetware.org/images/b/b2/DNA_design.jpg"></A></html> <html><A href=#TOP title="半白金　1μの動画"><IMG width="300px" src="http://openwetware.org/images/8/80/Damy.png"></A></html> <html><A href=#TOP title=DNA hybrudization><IMG width="300px"

src="http://openwetware.org/images/f/f1/DNA_hybrudization.jpg"></A></html>

<html><A href=#TOP title="ハイスピードカメラ"><IMG width="300px" src="http://openwetware.org/images/8/80/Damy.png"></A></html> <html><A href=#TOP><IMG width="300px" src="http://openwetware.org/images/e/ed/%E3%82%AB%E3%82%BF%E3%83%A9%E3%83%BC%E3%82%BC.jpg"></A></html> <html><A href=#TOP title="Azobenzene"><IMG width="300px" src="http://openwetware.org/images/e/ea/Azobenzene.jpg"></A></html>

Vapor deposition of Au and Cr on the polystyrene body

File:Body construction.png
    Deposition is a method for forming a metal thin film on the surface of the substrate.In this project, Gold and Chromium were deposited on the polystyrene surface in order to conjugate DNA onto the specific location on the surface.
>>see more Method

DNA conjugation

File:Body construction.png
SAM

    We bond Au with DNA using thiol.

>>see more Method

EDAC     We bond polystyrene-beads with DNA using EDAC.

>>see more Method

DNA Design

    We used NUPACK and designed five strands of DNA.

>>see more Method

Observation of platinum hemisphere

DNA hybridization in solution of H2O2

    We used PAGE electrophoresis to ascertain the stability of DNA duplex in thin H₂O₂ solution 1%~5%.

>>see more Method

Analysis of platinum by High-speed camera

Energy production by using catalase

Dissociation of azobenzene-modified DNA by UV-light irradiation

    Azobenzene including DNA can easily dissociate its duplex by irradiating UV-light. We put this switching system in JET body and enabled its control.

>>see more Method
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