Biomod/2012/Titech/Nano-Jugglers/Results: Difference between revisions
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:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/EDAC_conjugation|Method]] | :>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/EDAC_conjugation|Method]] | ||
|} | |} | ||
::After the protocol, to confirm that amino modified DNA is bind to polystyrene ,we hybridize the DNA with FAM and observe them under blue light by microscope.(Control experiment) | |||
:{| border="1" | |||
|'''Added Reagent | |||
|'''Polystyren beads'''<br>'''DNA which bind with beads'''<br>'''Complementary FAM''' | |||
|'''Polystyren beads'''<br>'''DNA which bind with beads'''<br><br> | |||
|'''Polystyren beads'''<br><br>'''Complementary FAM''' | |||
|- | |||
|'''Under Transmitted Light''' | |||
|[[Image:BIOMOD-TNJ-CrAu_1_2.jpg|250px]] | |||
|[[Image:BIOMOD-TNJ-CrAu_2.jpg|250px]] | |||
|[[Image:BIOMOD-TNJ-CrAu_3.jpg|250px]] | |||
|- | |||
|'''Under Blue Light''' | |||
|[[Image:BIOMOD-TNJ-CrAu_1_2_2s.jpg|250px]] | |||
|[[Image:BIOMOD-TNJ-CrAu_2_2s.jpg|250px]] | |||
|[[Image:BIOMOD-TNJ-CrAu_3_2s.jpg|250px]] | |||
|- | |||
|'''Fluorescence''' | |||
|'''observed''' | |||
|'''Not observed''' | |||
|'''Not observed''' | |||
|} | |||
[[Image:EDAC conjugation.jpg|400px]] | |||
:※We use polystyrene beads whose 1/4 was covered with Gold and 1/2 was covered with chromium.<br><br> | |||
:<big>From this result, we concluded that amino modified DNA is bind to polystyrene</big> | |||
===Observation Conditions=== | |||
:ISO6400<br> | |||
:Exposure time(Transmitted Light)1/100seconds<br> | |||
:Exposure time(Blue Light)2seconds<br> | |||
:Magnification 10×40=400<br> | |||
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div> | <div align = "right" style="padding-right:200px">[[#TOP|↑]]</div> |
Revision as of 00:52, 4 October 2012
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} </style> </head> <BODY> <div id="biomodlink"> <<a href="http://openwetware.org/wiki/Biomod">BIOMOD</a>|<a href="http://openwetware.org/wiki/Biomod/2012">2012</a>|Titech Nano-Jugglers </div> <div id="header"> <div id="navigation"> <div id="menu"> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers"><br>Home<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Team/Students"><br>Team<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Project"><br>Project<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Results">Results<br>&<br>Methods</a></font></li> <li class="ach"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Achievements"><br>Achievements<br><br></a> <li class="sup"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Protocols"><br>Suppl. Info.<br><br></a></li> <li class="none"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Acknowledgement"><br>Acknowledgements<br><br></a></li> </ul> </div> </div> </div> </BODY> </html>
We have set 3 goals, that is "Energy production for rail-free movement", "Speeding up", and "Direction control". |
Experimental results
<html><A href=#TOP title="Vapor deposition"><IMG width="300px" src="http://openwetware.org/images/a/a5/Vapor_deposition.jpg"></A></html> <html><A href=#TOP title="SAM conjugation"><IMG width="300px" src="http://openwetware.org/images/4/48/Thiol_.jpg"></A></html> <html><A href=#TOP title="EDAC conjugation"><IMG width="300px" src="http://openwetware.org/images/3/3d/EDAC_conjugation.jpg"></A></html> <html><A href=#TOP title="DNA design"><IMG width="300px" src="http://openwetware.org/images/b/b2/DNA_design.jpg"></A></html> <html><A href=#TOP title="半白金 1μの動画"><IMG width="300px" src="http://openwetware.org/images/8/80/Damy.png"></A></html> <html><A href=#TOP title=DNA hybrudization><IMG width="300px" src="http://openwetware.org/images/f/f1/DNA_hybrudization.jpg"></A></html>
<html><A href=#TOP title="ハイスピードカメラ"><IMG width="300px" src="http://openwetware.org/images/8/80/Damy.png"></A></html> <html><A href=#TOP><IMG width="300px" src="http://openwetware.org/images/e/ed/%E3%82%AB%E3%82%BF%E3%83%A9%E3%83%BC%E3%82%BC.jpg"></A></html> <html><A href=#TOP title="Azobenzene"><IMG width="300px" src="http://openwetware.org/images/e/ea/Azobenzene.jpg"></A></html>
Vapor deposition of Au and Cr on the polystyrene body
- >>see Method
- From these pictures, we observed that PVD can make hemisphere membrane of gold. By comparing the color of figure1 and figure2, micro-beads completely covered by gold are more black and have a metallic luster. From figure3 , it is possible that the size of 40μm beads can deposit gold as hemisphere in that the colors of beads sphere’s limbs are different .Figure 4 shows that 10 micron sized beads can also deposit gold as hemisphere, because metallic luster color and white color are dotted in one sphere.
DNA conjugation
File:Body construction.png
SAM conjugation
We bond Au with DNA using thiol.
- >>see more Method
- We observe that there were few signs anything after washing in ①,②,④ and ⑤.On the other hand, ③ and ⑥ were left water. This is expected that this spot of gold surface is covered with DNA by thiol conjugation, and became partially hydrophilic. Other spots are still hydrophobic in that there are no conjugations of DNA.
- From this result, we were determined that thiol conjugation is completed.
EDAC conjugation
We bond polystyrene-beads with DNA using EDAC.
- >>see more Method
- After the protocol, to confirm that amino modified DNA is bind to polystyrene ,we hybridize the DNA with FAM and observe them under blue light by microscope.(Control experiment)
- ※We use polystyrene beads whose 1/4 was covered with Gold and 1/2 was covered with chromium.
- From this result, we concluded that amino modified DNA is bind to polystyrene
Observation Conditions
- ISO6400
- Exposure time(Transmitted Light)1/100seconds
- Exposure time(Blue Light)2seconds
- Magnification 10×40=400
DNA Design
We used NUPACK and designed five strands of DNA.
- >>see more Method
Observation of platinum hemisphere
DNA hybridization in solution of H2O2
We used PAGE electrophoresis to ascertain the stability of DNA duplex in thin H₂O₂ solution 1%~5%.
- >>see more Method
- This image shows the observation results of DNA hybridization in solution of Hydrogen peroxide for 90minutes.From the picture, the hybridized DNA band appear in lane 1,2,3&8. In lane 4,5,6&7 appears single strand band. Lane1&8 are the same, lane 2 & 3 are added hydrogen peroxide solution (1% and 5% for 1h)for 90 minutes. In Lane 5&7 shows the observation results of single strand DNA (ssDNA) in solution of Hydrogen peroxide. In lane 4&6 are the control band of ssDNA.
- Upper white dotted line represents the same position of Lane 1&8 in that they are the same sample. Comparing between 4.5.6&7, these 4 lines appear lower than the white line, and 4-5and 6-7 are few differences. If hydrogen peroxide affects ssDNA, destroy, tear up or denature, line 5 and 7 will appear in the lower position or becomes unclear. Judging from appearances, differences between positive and negative control ware few.Comparing 1,2,3 and 8,these lines are completely appear in the white bar.Differences between these lines are only concentration of hydrogen peroxide. So, we conclude that there is no influence of hydrogen peroxide for DNA hybridizations however DNA is exposed to hydrogen peroxide within 90minutes.So, we could determine that there is no effect of hydrogen peroxide for dsDNA as well as ssDNA.
Analysis of platinum by High-speed camera
Energy production by using catalase
Dissociation of azobenzene-modified DNA by UV-light irradiation
Azobenzene including DNA can easily dissociate its duplex by irradiating UV-light. We put this switching system in JET body and enabled its control.
- >>see more Method