Biomod/2012/Titech/Nano-Jugglers/Project

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} </style> </head> <BODY> <div id="biomodlink"> <<a href="http://openwetware.org/wiki/Biomod">BIOMOD</a>|<a href="http://openwetware.org/wiki/Biomod/2012">2012</a>|Titech Nano-Jugglers </div> <div id="header"> <div id="navigation"> <div id="menu"> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers"><br>Home<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Team/Students"><br>Team<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Project"><br>Project<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Results">Results<br>&<br>Methods</a></font></li> <li class="ach"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Achievements"><br>Achievements<br><br></a> <li class="sup"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Protocols"><br>Suppl. Info.<br><br></a></li> <li class="none"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Acknowledgement"><br>Acknowledgements<br><br></a></li> </ul> </div> </div> </div> </BODY> </html>

Goals

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    Our goal is to build an unprecedented biomolecular rocket that achieves the following system functions.

  • Rail-free movement of a biomolecular rocket, whose journey is not restricted by the pre-determined rail
  • Extremely high-speed movement of a micrometer-sized biomolecular rocket, accomplished by accumulating nanometer-scale catalytic reactions
  • Directional control, based on a photo-switchable DNA system as a steering gear

Background

    Speeding-up is always on demand. In DNA nanotechnology, some synthetic molecular motors, such as a DNA spider, were proposed to execute nano- to micro-meter scale tasks including molecular tranportation. However, the speed of their movement is slow, compared to a kinesin that is one of the fastest natural molecular motor. Therefore, we decided to challenge the construction of a moleculer vehicle, that can exceed the speed of the kinesin.
    Among the vehicles that human beings have so far constructed, a rocket is the fastest one. In addition, a rocket can freely travel across the universe. So, we set our goal to construct a high-speed and remotely controllable biomolecular rocket that does not require any rail system, limiting the route of the rocket.
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Solution

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    A biomolecular rocket consists of a micrometer-sized bead as its body, submicrometer-sized beads or catalases as its catalytic engines, and DNA as its steering gear. The rail-free movement is accomplished by taking advantage of bubble propulsion like jet engines. To realize the high-speed movement of our rocket, we conjugated numerous catalytic engines by utilizing sequence-specific DNA hybridization. As a consquence, the directional control of our rocket movement is acheived by triggering region-specific DNA denaturation. As a proof of concept, we constructed a simple version of a bimomolecular rocket, as described below.

1. Power supply for the rail-free movement

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    Biomolecular rocket moves straightforward by emitting bubbles.

    We utilized the submicrometer-sized platinum beads or catalases that catalyze decomposition of H2O2 into H2O and O2. The generated O2 gas forms a bubble. The dissolved O2 gas surrounding a bubble continues to diffuse into the bubble, causing it to grow while the buoyancy force and surface adhesion compete against one another. When the O2 bubble is emitted from the surface of platinum beads, the momentum changes induces a driving force to put the bubble away from the surface. Therefore, by placing the catalytic engines on the back of the rocket body, the molecular rocket moves straightforward to the opposite direction, and thus, the rail-free movement is accomplished.

2. Increasing driving force for the high-speed movement

    Biomolecular rocket gets stronger driving force by increasing catalytic surface area.

    Since the driving force generated upon emission of bubbles depends on the surface area of catalyst, it is expected to speed our rocket up by increasing the surface area of platinum beads and enable it to emit more bubbles. For aiming at the high-speed record of the biomolecular rocket, we conjugated numerous catalytic engines to a micrometer-sized rocket body, instead of the conventional strategy to use a hemisphere of a bead as a rocket body for the catalytic suface.
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    In comparison, the surface area of a bead hemisphere, we can increase the surface area of platinum, therefore speed up in that we can increase the emission of O2 bubbles from the rocket.

3. Introduction of a photo-switchable DNA system for the directional control

    Direction of the rail-free movement of our rocket can be controlled, since we designed the photoresponsive DNA. Photoresponsive DNA structure is changed by UV light irradiation, then dissociation of double strand DNA will happen.
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    This reaction allows the detachment of the engines from the body upon the UV light irradiation. We designed all DNA strands that hybridization of each DNA are stable at room temperature, and only photoresponsive DNA can easily dissociate when UV irradiatting. By adjusting the position of photoresponsive DNA, we can control the direction of biomolecular rocket’s moving. We bond photoresponsive DNA with Au, and normal DNA with polystyrene and also each complementary DNAs with Pt.Difference in the amount of bubbles are generate at the position of the body. So, we can change the direction of our rocket by only irradiating UV light.
    Not only does Biomolecular Rocket have rail-free movement, we can control the directions, therefore we can say our rocket has a high-controlability.

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