Biomod/2012/Titech/Nano-Jugglers/Methods/Vapor Deposition: Difference between revisions

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=Physical Vapor Deposition Method=
=Vapor Deposition Method=


:'''>>Result page is [[Biomod/2012/Titech/Nano-Jugglers/Project/Results/Vapor_Deposition|here]]'''
:'''>>Result page is [[Biomod/2012/Titech/Nano-Jugglers/Results/Vapor_Deposition|here]]'''


==Overview==
==Overview==
::Deposition is a method for forming a metal thin film on the surface of the substrate.
    Deposition is a method for forming a thin metal film on the surface of the substrate. In this project, Gold and Chromium were deposited on the polystyrene beads in order to conjugate specific DNA onto the determined location on the surface.
::In this project, Gold and Chromium were deposited on the polystyrene surface in order to conjugate DNA onto the specific location on the surface.
==Vapor Deposition Method==
==Method==  
{|
:[[Image: BIOMODPVDM1.png]]
|
===1st Deposition===
===1st Deposition===
:First, we deposit gold on the polystyrene-bead and make bead, which are deposited by gold half.
::First, we deposit gold on the polystyrene beads in order to make gold hemisphered body.
===2nd Deposition===
===2nd Deposition===
:Next, we change the direction of the body, and deposit chromium on the body.
::Next, we change the direction of beads, and deposit chromium on the body.
:Then we pick up polystyrene-bead, which are deposited by quarter gold and half chromium.
::Then we pick up polystyrene-bead, which are deposited by quarter gold and half chromium.
===After PVD===
::By chromium capping, this "head part" is prevented to conjyugate DNA strands, so platinum only mounted at the rear.
:Through twice of physical vapor deposition,
===After Vapor Deposition===
::*we conjugate thiol modified DNA onto gold part.
::Through twice of vapor deposition,
::*we conjugate amino modified DNA onto polystyrene part.
:::*we conjugate thiol modified DNA onto gold part.
::*There are no DNA staple strands on chromium hemisphere.
:::*we conjugate amino modified DNA onto polystyrene part.
:Therefore, platinum particles can't conjugate onto chromium hemisphere.
:::*There are no DNA staple strands on chromium hemisphere.
:As a result, JET starts to move toward chromium hemisphere heading.
::Therefore, platinum particles can't conjugate onto chromium hemisphere.
::As a result, JET starts to move toward chromium hemisphere heading.
|}

Latest revision as of 23:01, 3 October 2012


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} </style> </head> <BODY> <div id="biomodlink"> <<a href="http://openwetware.org/wiki/Biomod">BIOMOD</a>|<a href="http://openwetware.org/wiki/Biomod/2012">2012</a>|Titech Nano-Jugglers </div> <div id="header"> <div id="navigation"> <div id="menu"> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers"><br>Home<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Team/Students"><br>Team<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Project"><br>Project<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Results">Results<br>&<br>Methods</a></font></li> <li class="ach"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Achievements"><br>Achievements<br><br></a> <li class="sup"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Protocols"><br>Suppl. Info.<br><br></a></li> <li class="none"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Acknowledgement"><br>Acknowledgements<br><br></a></li> </ul> </div> </div> </div> </BODY> </html>


Vapor Deposition Method

>>Result page is here

Overview

    Deposition is a method for forming a thin metal film on the surface of the substrate. In this project, Gold and Chromium were deposited on the polystyrene beads in order to conjugate specific DNA onto the determined location on the surface.

Vapor Deposition Method

1st Deposition

First, we deposit gold on the polystyrene beads in order to make gold hemisphered body.

2nd Deposition

Next, we change the direction of beads, and deposit chromium on the body.
Then we pick up polystyrene-bead, which are deposited by quarter gold and half chromium.
By chromium capping, this "head part" is prevented to conjyugate DNA strands, so platinum only mounted at the rear.

After Vapor Deposition

Through twice of vapor deposition,
  • we conjugate thiol modified DNA onto gold part.
  • we conjugate amino modified DNA onto polystyrene part.
  • There are no DNA staple strands on chromium hemisphere.
Therefore, platinum particles can't conjugate onto chromium hemisphere.
As a result, JET starts to move toward chromium hemisphere heading.
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