Biomod/2012/Titech/Nano-Jugglers/Methods/Vapor Deposition: Difference between revisions

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::In this project, Gold and Chromium were deposited on the polystyrene surface in order to conjugate DNA onto the specific location on the surface.
::In this project, Gold and Chromium were deposited on the polystyrene surface in order to conjugate DNA onto the specific location on the surface.
==Method==
==Method==
::Deposition is carried out in a vacuum.
 
::Vacuum state reduces the boiling point of the metal and makes it easy to deposition.<br>
Vacuum state reduces the boiling point of the metal and makes it easy to deposition.
::In this project, we use DNA for connection of Pt to polystyrene-beads.
In this project, we use DNA to connect platinum with the body.  
::It is possible to connect polystyrene-beads with DNA by method of EDAC.
===1st Deposition===
::However, when we are using only one type of DNA, it is impossible to complex control.
:First, we deposit gold on the polystyrene-bead and make bead, which are deposited by gold half.
::By depositing Au and Cr to polystyrene-beads, we were bound to different DNA position-specific.  
===2nd Deposition===
::And we were able to control photo-controlled JET more complex by this.
:Next, we change the direction of the body, and deposit chromium on the body.
:Then we pick up polystyrene-bead, which are deposited by quarter gold and half chromium.
===After PVD===
:Through twice of physical vapor deposition,
::*we conjugate thiol modified DNA onto gold part.
::*we conjugate amino modified DNA onto polystyrene part.
::*There are no DNA staple strands on chromium hemisphere.
:Therefore, platinum particles can't conjugate onto chromium hemisphere.
:As a result, JET starts to move toward chromium hemisphere heading.

Revision as of 06:21, 2 September 2012


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} </style> </head> <BODY> <div id="biomodlink"> <<a href="http://openwetware.org/wiki/Biomod">BIOMOD</a>|<a href="http://openwetware.org/wiki/Biomod/2012">2012</a>|Titech Nano-Jugglers </div> <div id="header"> <div id="navigation"> <div id="menu"> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers"><br>Home<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Team/Students"><br>Team<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Project"><br>Project<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Results">Results<br>&<br>Methods</a></font></li> <li class="ach"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Achievements"><br>Achievements<br><br></a> <li class="sup"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Protocols"><br>Suppl. Info.<br><br></a></li> <li class="none"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Acknowledgement"><br>Acknowledgements<br><br></a></li> </ul> </div> </div> </div> </BODY> </html>


Physical Vapor Deposition Method

>>Result page is here

Overview

Deposition is a method for forming a metal thin film on the surface of the substrate.
In this project, Gold and Chromium were deposited on the polystyrene surface in order to conjugate DNA onto the specific location on the surface.

Method

Vacuum state reduces the boiling point of the metal and makes it easy to deposition. In this project, we use DNA to connect platinum with the body.

1st Deposition

First, we deposit gold on the polystyrene-bead and make bead, which are deposited by gold half.

2nd Deposition

Next, we change the direction of the body, and deposit chromium on the body.
Then we pick up polystyrene-bead, which are deposited by quarter gold and half chromium.

After PVD

Through twice of physical vapor deposition,
  • we conjugate thiol modified DNA onto gold part.
  • we conjugate amino modified DNA onto polystyrene part.
  • There are no DNA staple strands on chromium hemisphere.
Therefore, platinum particles can't conjugate onto chromium hemisphere.
As a result, JET starts to move toward chromium hemisphere heading.
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