Biomod/2012/Titech/Nano-Jugglers/Methods/Polystyrene conjugation

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0.3. Selective DNA conjugation to the microbead body of Biomolecular Rocket

0.3.1. Selective conjugation of DNA to polystyrene surface area


    We bond polystyrene-beads with amino-modified DNA sequence L* with amino bond. For amino bond reactions, we use EDAC conjugation. EDAC is known as water-soluble carbodiimide (WSC) and is used as coupling agent to form amide bond. EDAC crosslinks nucleic acids to the surfaces, which have carboxy group. In this project, we used this method to conjugate amino-modified DNA onto carboxylated polystyrene-beads. EDAC reacted with carboxylated polystyrene-beads to form active intermediate on the body. Then it reacted to amino-modified DNA. The beads are coupled by nucleic acid. We divide the body into three parts including polystyrene surface, so it is possible to connect each DNA with position classification. By using this method, we were able to connect platinum to aircraft body part of polystyrene in a region-specific manner.

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