Biomod/2012/Titech/Nano-Jugglers/Methods/Metal area: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
 
(8 intermediate revisions by the same user not shown)
Line 1: Line 1:
{{Titech/Nano-Jugglers/HEAD}}
{{Titech/Nano-Jugglers/HEAD}}
==0.3. DNA conjugation==
==0.3. Selective DNA conjugation to the microbead body of Biomolecular Rocket==
===0.3.2. Selective conjugation of DNA to metal surface area===
===0.3.2. Selective conjugation of DNA to metal surface area===
<hr>
<hr>
Line 7: Line 7:
|<html><body><td align="center" width="150px"><img src="http://openwetware.org/images/0/01/BIOMODTNJSAM3.png" border=0 width=300 height=240></a></td></body></html>
|<html><body><td align="center" width="150px"><img src="http://openwetware.org/images/0/01/BIOMODTNJSAM3.png" border=0 width=300 height=240></a></td></body></html>
|
|
&nbsp;&nbsp;&nbsp;&nbsp;'''Self-assembled monolayers (SAM)<sup>[1],[2]</sup>''' of molecules are molecular assemblies formed spontaneously on substrate surfaces by chemical adsorption. This chemical reactions can be organized into large ordered domains and it is also possible to cover the substrate with functional end group that has some modification. In addition, few steps and experimental technique are required. SAMs are created by adsorption of head groups onto a substrate followed by a slow organization of tail groups.<br><br>
&nbsp;&nbsp;&nbsp;&nbsp;Selective conjugation of DNA to metal surface area is realized with Self-assembled monolayers (SAM)<sup>[1],[2]</sup>. SAM of molecules are molecular assemblies formed spontaneously on substrate surfaces by chemical adsorption. This chemical reactions can be organized into large ordered domains and it is also possible to cover the substrate with functional end group that has some modification. In addition, few steps and experimental technique are required. SAMs are created by adsorption of head groups onto a substrate followed by a slow organization of tail groups. First, head groups are absorbed onto substrate over. In this phase, the surface of substrate is patterned with spots by dsorbeate molecules rather than beautiful membranes.  
&nbsp;&nbsp;&nbsp;First, head groups are absorbed onto substrate over . In this phase, the surface of substrate is patterned with spots by dsorbeate molecules rather than beautiful membranes.  
|-
|-
|}
|}
Line 16: Line 15:
|<html><body><td align="center" width="150px"><img src="http://openwetware.org/images/4/48/Thiol_.jpg" border=0 width=300 height=240></a></td></body></html>
|<html><body><td align="center" width="150px"><img src="http://openwetware.org/images/4/48/Thiol_.jpg" border=0 width=300 height=240></a></td></body></html>
|
|
SAM conjugation onto gold<br>
DNA conjugation onto gold<br>
&nbsp;&nbsp;&nbsp;&nbsp;Thiolated compound and gold substrate are one of the most famous research of SAMs<sup>[3]</sup>. Thiol modified DNA is conjugate to gold particles by this chemical reaction. Initially, substrate gold are cleaned by water or acid-base buffer in order to washout organic impurities. Gold particles are dissolved in 10mM phosphate buffer pH8, 10μM thiol modified DNA. Then in addition to NaCl, intended to raise the concentration of NaCl every 2 hours after 24 hours of incubation for 6 hours, then incubate more 16 hoursraise up to 0.7M salt concentration '''(Salt aging process)'''.Last,clean up again by proper buffer and dry. This reaction is also use ​​with platinum    In order to accomplish our project ,we need platinum-DNA and gold-DNA conjugation, so we use thiol modified DNA.  
&nbsp;&nbsp;&nbsp;&nbsp;Thiolated DNA sequence S<sup>*</sup> compound and gold substrate are one of the most famous research of SAMs<sup>[3]</sup>. Thiol modified DNA was conjugate to gold particles by this chemical reaction. Initially, substrate gold were cleaned by water or acid-base buffer in order to washout organic impurities. Gold particles were dissolved in 10 mM phosphate buffer pH8, 10 μM thiol modified DNA.  
|-
|-
|}
|}

Latest revision as of 20:16, 27 October 2012


<html> <head> <style type="text/css"> /* ==================== 

  主に全体に関わるCSS

　　　==================== */ body.mediawiki { 

                       font-size: 14px;

background-image:url(http://openwetware.org/images/2/2d/TNJback.png); background-repeat: repeat; font-family: Calibri, Verdana, helvetica, sans-serif; } 

               h1 {
                       padding: 0px 20px 5px 20px;
                       font-size: 34px;
                       line-height: 40px;
                       font-weight: bold;
               }
               h2 {
                       padding: 20px 20px 5px 20px;
                       font-size: 25px;
                       color: #000000;
                       text-decoration: none;
                       font-weight: bold; 
              }
               h2 a {
                       color: #eb8300;
               }
               h3 {
                       padding: 20px 20px 5px 20px;
                       font-size: 20px;
                       color: #000;
                       font-decoration: none;
                       font-weight: bold;
               }
               h1.firstHeading {

display: none; 

               }

p { text-align: justify; } a:link { color: #00a5ea; text-decoration: none } a:visited { color:#00a5ea; text-decoration: none } a:hover { 

                       color: #eb8300;

text-decoration: none } a:active { color:#f29400; text-decoration: none } #content{ margin: 0px 0 0px 0; align: center; padding: 12px 12px 12px 12px; width: 946px; background-color: #ffff; border: 0; } #bodyContent{ width: 970px; margin: 150px auto 0 auto; align: center; background-color: #ffffff; } #globalWrapper{ margin: 20px 0 0 0; width:994px; background-color: #ffffff; margin-left: auto; margin-right: auto } #biomodlink{ position:absolute;top:0px; width:970px;height:20px; background-color: #ffffff; margin:3px auto 3px auto }


/* ==================== 

  メニューの画像を変更できる部分
  ==================== */

#header { 

                       margin: 0 auto 0 auto;
                       position:absolute;
                       top:28px; width:968px;height:189px;

background-color: #FFFFFF; background-repeat: no-repeat; 

                       background-image: url(http://openwetware.org/images/7/74/Title-LOGO6.jpg);

} /* ==================== 

  以下、特殊なclassに適用される
  ==================== */

#navigation { position:absolute; 

                       top:120px; width:970px; height:92px;
                       background-repeat: no-repeat;

color: #0000FF; }

/* ==================== 

  ここからプルダウン周辺のデザイン
  ==================== */
  1. menu * {

margin: 0; padding: 0; }

  1. menu {
       position:absolute;

font-family: calibri, verdana, sans-serif; 

       font-color: #000000;

background-color: transparent; float:left; display: block; }

  1. menu ul {
       position:relative;

float: left; text-align:center; list-style: none; }

  1. menu li {

background-color:yellow; 

       position:relative;

float:left; 

       width: 136px;

font-size: 23px; 

       font-weight: bold;

border:ridge 0px #FFFFFF; }

  1. menu li.ach {

background-color:yellow; 

       position:relative;

float:left; 

       width: 162px;

font-size: 23px; 

       font-weight: bold;

border:ridge 0px #FFFFFF; }

  1. menu li.sup {

background-color:yellow; 

       position:relative;

float:left; 

       width: 109px;

font-size: 17px; 

       font-weight: bold;

border:ridge 0px #FFFFFF; }

  1. menu li.none {

background-color:yellow; 

       position:relative;

float:left; 

       width: 152px;

font-size: 17px; 

       font-weight: bold;

border:ridge 0px #FFFFFF; }

  1. menu a {

color: #000000; display:block; text-decoration: none; }

  1. menu a:hover {

background-color:gold; }

  1. column-one { display:none; width:0px;}

.container{background-color: #ffffff; margin-top:0px} .OWWNBcpCurrentDateFilled {display: none;}

  1. footer{position: center; width: 994px}

@media screen { 

   body { background: #F5F5F5 0 0 no-repeat;  /* changed default background */ }

} </style> </head> <BODY> <div id="biomodlink"> <<a href="http://openwetware.org/wiki/Biomod">BIOMOD</a>|<a href="http://openwetware.org/wiki/Biomod/2012">2012</a>|Titech Nano-Jugglers </div> <div id="header"> <div id="navigation"> <div id="menu"> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers"><br>Home<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Team/Students"><br>Team<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Project"><br>Project<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Results">Results<br>&<br>Methods</a></font></li> <li class="ach"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Achievements"><br>Achievements<br><br></a> <li class="sup"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Protocols"><br>Suppl. Info.<br><br></a></li> <li class="none"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Acknowledgement"><br>Acknowledgements<br><br></a></li> </ul> </div> </div> </div> </BODY> </html>

0.3. Selective DNA conjugation to the microbead body of Biomolecular Rocket

0.3.2. Selective conjugation of DNA to metal surface area

<html><body><td align="center" width="150px"><img src="http://openwetware.org/images/0/01/BIOMODTNJSAM3.png" border=0 width=300 height=240></a></td></body></html>

    Selective conjugation of DNA to metal surface area is realized with Self-assembled monolayers (SAM)[1],[2]. SAM of molecules are molecular assemblies formed spontaneously on substrate surfaces by chemical adsorption. This chemical reactions can be organized into large ordered domains and it is also possible to cover the substrate with functional end group that has some modification. In addition, few steps and experimental technique are required. SAMs are created by adsorption of head groups onto a substrate followed by a slow organization of tail groups. First, head groups are absorbed onto substrate over. In this phase, the surface of substrate is patterned with spots by dsorbeate molecules rather than beautiful membranes.

   Over the period of disordering form, the head groups assemble together on the substrate, while the tail groups assemble far from the substrate. Areas of concentrated molecules nucleate and grow until the surface of the substrate is covered in a single monolayer.
<html><body><td align="center" width="150px"><img src="http://openwetware.org/images/4/48/Thiol_.jpg" border=0 width=300 height=240></a></td></body></html>

DNA conjugation onto gold
    Thiolated DNA sequence S* compound and gold substrate are one of the most famous research of SAMs[3]. Thiol modified DNA was conjugate to gold particles by this chemical reaction. Initially, substrate gold were cleaned by water or acid-base buffer in order to washout organic impurities. Gold particles were dissolved in 10 mM phosphate buffer pH8, 10 μM thiol modified DNA.

Reference
[1] A. Ulman, Chem Rev 96, 1533 (1996).
[2] G. E. Poirier, Chem Rev 97, 1117 (1997).
[3]Dmitri Y. Petrovykh,; Hiromi Kimura-Suda,; Aric Opdahl,; Lee J. Richter,; Michael J. Tarlov,; Lloyd J. Whitman.Langmuir 22, 2578-2587(2006)
>>back to Results
Retrieved from "https://openwetware.org/mediawiki/index.php?title=Biomod/2012/Titech/Nano-Jugglers/Methods/Metal_area&oldid=647797"