Biomod/2012/Titech/Nano-Jugglers/Methods/Dissociation of photoresponsive DNA - Revision history

Kensuke Hoshi: /* 3.2. Achievement of the photo-switchable DNA system */

Kensuke Hoshi — Sun, 28 Oct 2012 06:39:23 GMT

3.2. Achievement of the photo-switchable DNA system

←Older revision		Revision as of 06:39, 28 October 2012
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	The duplex-forming activities of DNA can be photomodulated by incorporation of an azobenzene unit<sup>[1]</sup>. The duplex is dissociated on isomerizing the trans-azobenzene to the cis form by irradiation with UV light. The duplex is formed again when the cis-azobenzene is converted to the transazobenzene by irradiation with visible light. <br>		The duplex-forming activities of DNA can be photomodulated by incorporation of an azobenzene unit<sup>[1]</sup>. The duplex is dissociated on isomerizing the trans-azobenzene to the cis form by irradiation with UV light. The duplex is formed again when the cis-azobenzene is converted to the transazobenzene by irradiation with visible light. <br>
-	We inserted this function into a bead-body and ~~add~~ the photo-switching function to the ~~body~~.In brief, so that we can ~~detached~~ the engines by irradiation with UV light, we attached ~~a part of~~ engines to the body with DNA duplex incorporating azobenzene unit.	+	We inserted this function into a bead-body and added the photo-switching function to the Biomolecular Rocket.In brief, so that we can detach the engines by irradiation with UV light, we attached engines to the body with DNA duplex incorporating azobenzene unit.
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-	:    We measured DNA absorbance and ascertain the duplex-forming and duplex-dissociation activities of photoresponsive DNA sequence S and S<sup>*</sup>. As the ordered regions of stacked base pairs in the DNA duplex are dissociated, the UV absorbance increases. This difference in absorbance between the duplex and single strand state is the result of nearest neighbor base pair interactions. In other words, when the DNA is in the duplex state, interactions between base pairs decrease the UV absorbance relative to single strands. When the DNA is in the single strand state the interactions are much weaker,due to the decreased proximity, and the UV absorbance is higher than the duplex state. About this time, we analyze the ~~rerationship~~ between the time of dissociation photo-switcable DNA and the strength of the UV light. <br>	+	:    We measured DNA absorbance and ascertain the duplex-forming and duplex-dissociation activities of photoresponsive DNA sequence S and S<sup>*</sup>. As the ordered regions of stacked base pairs in the DNA duplex are dissociated, the UV absorbance increases. This difference in absorbance between the duplex and single strand state is the result of nearest neighbor base pair interactions. In other words, when the DNA is in the duplex state, interactions between base pairs decrease the UV absorbance relative to single strands. When the DNA is in the single strand state the interactions are much weaker,due to the decreased proximity, and the UV absorbance is higher than the duplex state. About this time, we analyze the relationship between the time for dissociation of photo-switcable DNA and the strength of the UV light. <br>
	:Reference		:Reference
	:[1]Xingguo Liang,Hidenori Nishioka, Nobutaka Takenaka, and Hiroyuki Asanuma,;ChemBioChem 9, 702 – 705(2008)		:[1]Xingguo Liang,Hidenori Nishioka, Nobutaka Takenaka, and Hiroyuki Asanuma,;ChemBioChem 9, 702 – 705(2008)
	::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3.2._Achievement_of_the_photo-switchable_DNA_system\|Results]]		::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3.2._Achievement_of_the_photo-switchable_DNA_system\|Results]]

Kensuke Hoshi: /* 3.2. Directional control with photo-switchable DNA system */

Kensuke Hoshi — Sun, 28 Oct 2012 06:01:24 GMT

3.2. Directional control with photo-switchable DNA system

←Older revision		Revision as of 06:01, 28 October 2012
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	{{Titech/Nano-Jugglers/HEAD}}		{{Titech/Nano-Jugglers/HEAD}}
-	==3.2. ~~Directional control with~~ photo-switchable DNA system==	+	==3.2. Achievement of the photo-switchable DNA system==
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Kensuke Hoshi: /* 3.2. Directional control with photo-switchable DNA system */

Kensuke Hoshi — Sun, 28 Oct 2012 06:00:46 GMT

3.2. Directional control with photo-switchable DNA system

←Older revision		Revision as of 06:00, 28 October 2012
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	:Reference		:Reference
	:[1]Xingguo Liang,Hidenori Nishioka, Nobutaka Takenaka, and Hiroyuki Asanuma,;ChemBioChem 9, 702 – 705(2008)		:[1]Xingguo Liang,Hidenori Nishioka, Nobutaka Takenaka, and Hiroyuki Asanuma,;ChemBioChem 9, 702 – 705(2008)
-	::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3.2.~~_Directional_control_with_photo~~-switchable_DNA_system\|Results]]	+	::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3.2._Achievement_of_the_photo-switchable_DNA_system\|Results]]

Kensuke Hoshi: /* 3.2. Directional control with photo-switchable DNA system */

Kensuke Hoshi — Sun, 28 Oct 2012 06:00:05 GMT

3.2. Directional control with photo-switchable DNA system

←Older revision		Revision as of 06:00, 28 October 2012
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	:Reference		:Reference
	:[1]Xingguo Liang,Hidenori Nishioka, Nobutaka Takenaka, and Hiroyuki Asanuma,;ChemBioChem 9, 702 – 705(2008)		:[1]Xingguo Liang,Hidenori Nishioka, Nobutaka Takenaka, and Hiroyuki Asanuma,;ChemBioChem 9, 702 – 705(2008)
-	::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3.2.~~_ Directional_control_with_photo~~-switchable_DNA_system\|Results]]	+	::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3.2._Directional_control_with_photo-switchable_DNA_system\|Results]]

Kensuke Hoshi: /* 3.2. Directional control with photo-switchable DNA system */

Kensuke Hoshi — Sun, 28 Oct 2012 04:35:23 GMT

3.2. Directional control with photo-switchable DNA system

←Older revision		Revision as of 04:35, 28 October 2012
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	We inserted this function into a bead-body and add the photo-switching function to the body.In brief, so that we can detached the engines by irradiation with UV light, we attached a part of engines to the body with DNA duplex incorporating azobenzene unit.		We inserted this function into a bead-body and add the photo-switching function to the body.In brief, so that we can detached the engines by irradiation with UV light, we attached a part of engines to the body with DNA duplex incorporating azobenzene unit.
	\|}		\|}
-	:    We measured DNA absorbance and ascertain the duplex-forming and duplex-dissociation activities of ~~photoswitcable~~ DNA ~~strands~~. As the ordered regions of stacked base pairs in the DNA duplex are dissociated, the UV absorbance increases. This difference in absorbance between the duplex and single strand state is the result of nearest neighbor base pair interactions. In other words, when the DNA is in the duplex state, interactions between base pairs decrease the UV absorbance relative to single strands. When the DNA is in the single strand state the interactions are much weaker,due to the decreased proximity, and the UV absorbance is higher than the duplex state. About this time, we analyze the rerationship between the time of dissociation photo-switcable DNA and the strength of the UV light. <br>	+	:    We measured DNA absorbance and ascertain the duplex-forming and duplex-dissociation activities of photoresponsive DNA sequence S and S<sup>*</sup>. As the ordered regions of stacked base pairs in the DNA duplex are dissociated, the UV absorbance increases. This difference in absorbance between the duplex and single strand state is the result of nearest neighbor base pair interactions. In other words, when the DNA is in the duplex state, interactions between base pairs decrease the UV absorbance relative to single strands. When the DNA is in the single strand state the interactions are much weaker,due to the decreased proximity, and the UV absorbance is higher than the duplex state. About this time, we analyze the rerationship between the time of dissociation photo-switcable DNA and the strength of the UV light. <br>
	:Reference		:Reference
	:[1]Xingguo Liang,Hidenori Nishioka, Nobutaka Takenaka, and Hiroyuki Asanuma,;ChemBioChem 9, 702 – 705(2008)		:[1]Xingguo Liang,Hidenori Nishioka, Nobutaka Takenaka, and Hiroyuki Asanuma,;ChemBioChem 9, 702 – 705(2008)
	::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3.2._ Directional_control_with_photo-switchable_DNA_system\|Results]]		::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3.2._ Directional_control_with_photo-switchable_DNA_system\|Results]]

Kensuke Hoshi: /* 3.2. Dissociation of photoresponsive DNA by UV-light irradiation */

Kensuke Hoshi — Fri, 26 Oct 2012 09:44:42 GMT

3.2. Dissociation of photoresponsive DNA by UV-light irradiation

←Older revision		Revision as of 09:44, 26 October 2012
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	{{Titech/Nano-Jugglers/HEAD}}		{{Titech/Nano-Jugglers/HEAD}}
-	==3.2. ~~Dissociation of photoresponsive DNA by UV~~-~~light irradiation~~==	+	==3.2. Directional control with photo-switchable DNA system==
	:{\|		:{\|
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	:Reference		:Reference
	:[1]Xingguo Liang,Hidenori Nishioka, Nobutaka Takenaka, and Hiroyuki Asanuma,;ChemBioChem 9, 702 – 705(2008)		:[1]Xingguo Liang,Hidenori Nishioka, Nobutaka Takenaka, and Hiroyuki Asanuma,;ChemBioChem 9, 702 – 705(2008)
-	::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3.2.~~_Dissociation_of_photoresponsive_DNA_by_UV~~-~~light_irradiation~~\|Results]]	+	::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3.2._ Directional_control_with_photo-switchable_DNA_system\|Results]]

Kensuke Hoshi: /* 3.2. Dissociation of photoresponsive DNA by UV-light irradiation */

Kensuke Hoshi — Fri, 26 Oct 2012 06:16:12 GMT

3.2. Dissociation of photoresponsive DNA by UV-light irradiation

←Older revision		Revision as of 06:16, 26 October 2012
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	:    We measured DNA absorbance and ascertain the duplex-forming and duplex-dissociation activities of photoswitcable DNA strands. As the ordered regions of stacked base pairs in the DNA duplex are dissociated, the UV absorbance increases. This difference in absorbance between the duplex and single strand state is the result of nearest neighbor base pair interactions. In other words, when the DNA is in the duplex state, interactions between base pairs decrease the UV absorbance relative to single strands. When the DNA is in the single strand state the interactions are much weaker,due to the decreased proximity, and the UV absorbance is higher than the duplex state. About this time, we analyze the rerationship between the time of dissociation photo-switcable DNA and the strength of the UV light. <br>		:    We measured DNA absorbance and ascertain the duplex-forming and duplex-dissociation activities of photoswitcable DNA strands. As the ordered regions of stacked base pairs in the DNA duplex are dissociated, the UV absorbance increases. This difference in absorbance between the duplex and single strand state is the result of nearest neighbor base pair interactions. In other words, when the DNA is in the duplex state, interactions between base pairs decrease the UV absorbance relative to single strands. When the DNA is in the single strand state the interactions are much weaker,due to the decreased proximity, and the UV absorbance is higher than the duplex state. About this time, we analyze the rerationship between the time of dissociation photo-switcable DNA and the strength of the UV light. <br>
		+	:Reference
	:[1]Xingguo Liang,Hidenori Nishioka, Nobutaka Takenaka, and Hiroyuki Asanuma,;ChemBioChem 9, 702 – 705(2008)		:[1]Xingguo Liang,Hidenori Nishioka, Nobutaka Takenaka, and Hiroyuki Asanuma,;ChemBioChem 9, 702 – 705(2008)
-	::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3.2._Dissociation_of_photoresponsive_DNA_by_UV-light_irradiation\|~~Result~~]]	+	::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3.2._Dissociation_of_photoresponsive_DNA_by_UV-light_irradiation\|Results]]

Kensuke Hoshi: /* 3.2. Dissociation of photoresponsive DNA by UV-light irradiation */

Kensuke Hoshi — Fri, 26 Oct 2012 06:15:23 GMT

3.2. Dissociation of photoresponsive DNA by UV-light irradiation

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-	\|<html><body><td align="center" width="150px"><img src="http://openwetware.org/images/6/6f/~~Photo_switching_system~~.jpg" border=0 width=300 height=240></a></td></body></html>	+	\|<html><body><td align="center" width="150px"><img src="http://openwetware.org/images/7/74/Azoazo.jpg" border=0 width=300 height=240></a></td></body></html>
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	The duplex-forming activities of DNA can be photomodulated by incorporation of an azobenzene unit<sup>[1]</sup>. The duplex is dissociated on isomerizing the trans-azobenzene to the cis form by irradiation with UV light. The duplex is formed again when the cis-azobenzene is converted to the transazobenzene by irradiation with visible light. <br>		The duplex-forming activities of DNA can be photomodulated by incorporation of an azobenzene unit<sup>[1]</sup>. The duplex is dissociated on isomerizing the trans-azobenzene to the cis form by irradiation with UV light. The duplex is formed again when the cis-azobenzene is converted to the transazobenzene by irradiation with visible light. <br>

Kensuke Hoshi: /* 3-2.Dissociation of photoresponsive DNA by UV-light irradiation */

Kensuke Hoshi — Wed, 24 Oct 2012 09:04:08 GMT

3-2.Dissociation of photoresponsive DNA by UV-light irradiation

←Older revision		Revision as of 09:04, 24 October 2012
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	{{Titech/Nano-Jugglers/HEAD}}		{{Titech/Nano-Jugglers/HEAD}}
-	==3-2.Dissociation of photoresponsive DNA by UV-light irradiation==	+	==3.2. Dissociation of photoresponsive DNA by UV-light irradiation==
	:{\|		:{\|
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	:    We measured DNA absorbance and ascertain the duplex-forming and duplex-dissociation activities of photoswitcable DNA strands. As the ordered regions of stacked base pairs in the DNA duplex are dissociated, the UV absorbance increases. This difference in absorbance between the duplex and single strand state is the result of nearest neighbor base pair interactions. In other words, when the DNA is in the duplex state, interactions between base pairs decrease the UV absorbance relative to single strands. When the DNA is in the single strand state the interactions are much weaker,due to the decreased proximity, and the UV absorbance is higher than the duplex state. About this time, we analyze the rerationship between the time of dissociation photo-switcable DNA and the strength of the UV light. <br>		:    We measured DNA absorbance and ascertain the duplex-forming and duplex-dissociation activities of photoswitcable DNA strands. As the ordered regions of stacked base pairs in the DNA duplex are dissociated, the UV absorbance increases. This difference in absorbance between the duplex and single strand state is the result of nearest neighbor base pair interactions. In other words, when the DNA is in the duplex state, interactions between base pairs decrease the UV absorbance relative to single strands. When the DNA is in the single strand state the interactions are much weaker,due to the decreased proximity, and the UV absorbance is higher than the duplex state. About this time, we analyze the rerationship between the time of dissociation photo-switcable DNA and the strength of the UV light. <br>
	:[1]Xingguo Liang,Hidenori Nishioka, Nobutaka Takenaka, and Hiroyuki Asanuma,;ChemBioChem 9, 702 – 705(2008)		:[1]Xingguo Liang,Hidenori Nishioka, Nobutaka Takenaka, and Hiroyuki Asanuma,;ChemBioChem 9, 702 – 705(2008)
-	::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3-2.~~Dissociation_of_photoresponsive_DNA_by_UV~~-light_irradiation\|Result]]	+	::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3.2._Dissociation_of_photoresponsive_DNA_by_UV-light_irradiation\|Result]]

Kensuke Hoshi: /* 3-2.Dissociation of photoresponsive DNA by UV-light irradiation */

Kensuke Hoshi — Tue, 23 Oct 2012 03:29:16 GMT

3-2.Dissociation of photoresponsive DNA by UV-light irradiation

←Older revision		Revision as of 03:29, 23 October 2012
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	:    We measured DNA absorbance and ascertain the duplex-forming and duplex-dissociation activities of photoswitcable DNA strands. As the ordered regions of stacked base pairs in the DNA duplex are dissociated, the UV absorbance increases. This difference in absorbance between the duplex and single strand state is the result of nearest neighbor base pair interactions. In other words, when the DNA is in the duplex state, interactions between base pairs decrease the UV absorbance relative to single strands. When the DNA is in the single strand state the interactions are much weaker,due to the decreased proximity, and the UV absorbance is higher than the duplex state. About this time, we analyze the rerationship between the time of dissociation photo-switcable DNA and the strength of the UV light. <br>		:    We measured DNA absorbance and ascertain the duplex-forming and duplex-dissociation activities of photoswitcable DNA strands. As the ordered regions of stacked base pairs in the DNA duplex are dissociated, the UV absorbance increases. This difference in absorbance between the duplex and single strand state is the result of nearest neighbor base pair interactions. In other words, when the DNA is in the duplex state, interactions between base pairs decrease the UV absorbance relative to single strands. When the DNA is in the single strand state the interactions are much weaker,due to the decreased proximity, and the UV absorbance is higher than the duplex state. About this time, we analyze the rerationship between the time of dissociation photo-switcable DNA and the strength of the UV light. <br>
-	:Xingguo Liang,Hidenori Nishioka, Nobutaka Takenaka, and Hiroyuki Asanuma,;ChemBioChem 9, 702 – 705(2008)	+	:[1]Xingguo Liang,Hidenori Nishioka, Nobutaka Takenaka, and Hiroyuki Asanuma,;ChemBioChem 9, 702 – 705(2008)
	::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3-2.Dissociation_of_photoresponsive_DNA_by_UV-light_irradiation\|Result]]		::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3-2.Dissociation_of_photoresponsive_DNA_by_UV-light_irradiation\|Result]]