Biomod/2012/Titech/Nano-Jugglers/Methods/Design of photoresponsive DNA

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(New page: {{Titech/Nano-Jugglers/HEAD}} ==3-1.Design of photoresponsive DNA== :    We incorporated 4 azobenzene units into photoresponsive DNA strand so that we can quickly disso...)
(3-1.Design of photoresponsive DNA)
Line 1: Line 1:
{{Titech/Nano-Jugglers/HEAD}}
{{Titech/Nano-Jugglers/HEAD}}
==3-1.Design of photoresponsive DNA==
==3-1.Design of photoresponsive DNA==
-
:    We incorporated 4 azobenzene units into photoresponsive DNA strand so that we can quickly dissociated DNA duplex.{Photo-switchable DNA 5'-AATXACXCCXAGXCC-3' (X=Azobenzene)}
+
:&nbsp;&nbsp;&nbsp;&nbsp;We incorporated 4 azobenzene units into photoresponsive DNA<sup>[1]</sup> strand so that we can quickly dissociated DNA duplex.{Photo-switchable DNA 5'-AATXACXCCXAGXCC-3' (X=Azobenzene)}
:{|
:{|
|-
|-
Line 19: Line 19:
:[[Image:Math.png|center|200px]]
:[[Image:Math.png|center|200px]]
:&nbsp;&nbsp;&nbsp;&nbsp;(Abs: absorbance, ε: the absorption coefficient, c: strength, d: length of a visual leg )  
:&nbsp;&nbsp;&nbsp;&nbsp;(Abs: absorbance, ε: the absorption coefficient, c: strength, d: length of a visual leg )  
-
:&nbsp;&nbsp;&nbsp;&nbsp;The value of Abs is proportional to the concentration of corresponding materials. If photoresponsive DNA formed duplex, the concentration of ssDNA decrease, onthe other hand the concentration of dsDNA increase. Reserchin these corresponding values, we could determine whether they formed duplex or not.  
+
:&nbsp;&nbsp;&nbsp;&nbsp;The value of Abs is proportional to the concentration of corresponding materials. If photoresponsive DNA formed duplex, the concentration of ssDNA decrease, onthe other hand the concentration of dsDNA increase. Reserchin these corresponding values, we could determine whether they formed duplex or not.<br>
 +
:[1]Hiroyuki Asanuma, Xingguo Liang, Takayuki Yoshida, and Makoto Komiyama,;CHEMBIOCHEM  2, 39-44(2001)
::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3-1.Design_of_photoresponsive_DNA|Result]]
::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#3-1.Design_of_photoresponsive_DNA|Result]]

Revision as of 22:26, 22 October 2012


3-1.Design of photoresponsive DNA

    We incorporated 4 azobenzene units into photoresponsive DNA[1] strand so that we can quickly dissociated DNA duplex.{Photo-switchable DNA 5'-AATXACXCCXAGXCC-3' (X=Azobenzene)}

photoresponsive DNA

  • 5’ -(HS)-AATxACxCCxAGxCC-3’ (x=azobenzene)

1.We incorporated 4 azobenzene units into 11 bases DNA so that we can quickly dissociated DNA duplex.

cDNA of photoresponsive DNA

  • 5’-(HS)-GGCTGGGTATT-3’

1.This 11 bases DNA are a complementary sequence of DNAⅰ(photoresponsive DNA).

    And then, we confirmed that photoresponsive DNA could hybridize stably at consistent temperature. For verifying hybridization, we use the value of absorbance.
    In spectroscopy, a relational expression about absorbance can be expressed as follows.
    (Abs: absorbance, ε: the absorption coefficient, c: strength, d: length of a visual leg )
    The value of Abs is proportional to the concentration of corresponding materials. If photoresponsive DNA formed duplex, the concentration of ssDNA decrease, onthe other hand the concentration of dsDNA increase. Reserchin these corresponding values, we could determine whether they formed duplex or not.
[1]Hiroyuki Asanuma, Xingguo Liang, Takayuki Yoshida, and Makoto Komiyama,;CHEMBIOCHEM 2, 39-44(2001)
>>back to Result
Personal tools