Biomod/2012/Titech/Nano-Jugglers/Methods: Difference between revisions

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<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div>
==Physical Vapor Deposition==
==Vapor deposition of Au and Cr on the polystyrene body==
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&nbsp;&nbsp;&nbsp;&nbsp;Deposition is a method for forming a metal thin film on the surface of the substrate.In this project, Gold and Chromium were deposited on the polystyrene surface in order to conjugate DNA onto the specific location on the surface.
&nbsp;&nbsp;&nbsp;&nbsp;Deposition is a method for forming a metal thin film on the surface of the substrate.In this project, Gold and Chromium were deposited on the polystyrene surface in order to conjugate DNA onto the specific location on the surface.

Revision as of 22:09, 28 September 2012


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} </style> </head> <BODY> <div id="biomodlink"> <<a href="http://openwetware.org/wiki/Biomod">BIOMOD</a>|<a href="http://openwetware.org/wiki/Biomod/2012">2012</a>|Titech Nano-Jugglers </div> <div id="header"> <div id="navigation"> <div id="menu"> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers"><br>Home<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Team/Students"><br>Team<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Project"><br>Project<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Results">Results<br>&<br>Methods</a></font></li> <li class="ach"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Achievements"><br>Achievements<br><br></a> <li class="sup"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Protocols"><br>Suppl. Info.<br><br></a></li> <li class="none"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Acknowledgement"><br>Acknowledgements<br><br></a></li> </ul> </div> </div> </div> </BODY> </html>


Methods

<html><A href=#TOP title="SAM"><IMG width="300px" src="http://openwetware.org/images/4/48/Thiol_.jpg"></A></html> <html><A href=#TOP><IMG width="300px" src="http://openwetware.org/images/d/da/BIOMOD-TNJ-CrAu_1_2_2s-13.jpg"></A></html> <html><A href=#TOP><IMG width="300px" src="http://openwetware.org/images/f/fa/BIOMODTNJPVDR4.jpg"></A></html>
<html><A href=#TOP><IMG width="300px" src="http://openwetware.org/images/e/ea/BIOMOD-TNJ-azobenzen8.png"></A></html> <html><A href=#TOP><IMG width="300px" src="http://openwetware.org/images/3/30/BIOMODTNJEPR_TITLE.JPG"></A></html> <html><A href=#TOP><IMG width="300px" src="http://openwetware.org/images/1/19/255247_melt.png"></A></html>
<html><A href=#TOP><IMG width="300px" src="http://openwetware.org/images/8/80/Damy.png"></A></html> <html><A href=#TOP><IMG width="300px" src="http://openwetware.org/images/8/80/Damy.png"></A></html> <html><A href=#TOP><IMG width="300px" src="http://openwetware.org/images/8/80/Damy.png"></A></html>

DNA conjugation

    We bond Au with DNA using thiol.

>>see more Method
>>see Result

    We bond polystyrene-beads with DNA using EDAC.

>>see more Method
>>see Result

Vapor deposition of Au and Cr on the polystyrene body

    Deposition is a method for forming a metal thin film on the surface of the substrate.In this project, Gold and Chromium were deposited on the polystyrene surface in order to conjugate DNA onto the specific location on the surface.

>>see more Method
>>see Result

Azobenzene Tweezers

    Azobenzene including DNA can easily dissociate its duplex by irradiating UV-light. We put this switching system in JET body and enabled its control.

>>see more Method
>>see Result

DNA hybridization in solution of H2O2

    We used PAGE electrophoresis to ascertain the stability of DNA duplex in thin H₂O₂ solution 1%~5%.

>>see more Method
>>see Result

DNA Design

    We used NUPACK and designed five strands of DNA.

>>see more Method
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