Biomod/2012/Titech/Nano-Jugglers/Methods: Difference between revisions
No edit summary |
No edit summary |
||
Line 5: | Line 5: | ||
==Methods== | ==Methods== | ||
==DNA conjugation== | |||
:{| | |||
|- | |||
|width="300px"|[[Image:Nano-jugglers_Thiol.jpg|center|300px]] | |||
| | |||
We bond Au with DNA using thiol. | |||
:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/Thiol_conjugation|Method]] | |||
:>>see [[Biomod/2012/Titech/Nano-Jugglers/Results/Thiol_conjugation|Result]] | |||
|} | |||
:{| | |||
|- | |||
|width="300px"|[[Image:BIOMOD-TNJ-CrAu_1_2_2s-13.jpg|center|300px]] | |||
| | |||
We bond polystyrene-beads with DNA using EDAC. | |||
:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/EDAC_conjugation|Method]] | |||
:>>see [[Biomod/2012/Titech/Nano-Jugglers/Results/EDAC_conjugation|Result]] | |||
|} | |||
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div> | |||
==Physical Vapor Deposition== | |||
:{| | |||
|- | |||
|width="300px"|[[Image:BIOMODTNJPVDR4.jpg|300px]] | |||
| | |||
Deposition is a method for forming a metal thin film on the surface of the substrate.In this project, Gold and Chromium were deposited on the polystyrene surface in order to conjugate DNA onto the specific location on the surface. | |||
:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/Vapor_Deposition|Method]] | |||
:>>see [[Biomod/2012/Titech/Nano-Jugglers/Results/Vapor_Deposition|Result]] | |||
|} | |||
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div> | |||
==Azobenzene Tweezers== | |||
:{|[[ | |||
|- | |||
|width="300px"|[[Image:BIOMOD-TNJ-azobenzen8.png|300px|center]] | |||
| | |||
Azobenzene including DNA can easily dissociate its duplex by irradiating UV-light. We put this switching system in JET body and enabled its control. | |||
:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/Azobenzene_Tweezer|Method]] | |||
:>>see [[Biomod/2012/Titech/Nano-Jugglers/Results/Azobenzene_Tweezer|Result]] | |||
|} | |||
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div> | |||
==DNA hybridization in solution of H<small>2</small>O<small>2</small>== | |||
:{| | |||
|- | |||
|width="300px"|[[Image:BIOMODTNJEPR TITLE.JPG|center|300px]] | |||
| | |||
We used PAGE electrophoresis to ascertain the stability of DNA duplex in thin H₂O₂ solution 1%~5%. | |||
:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/DNA_hybridization|Method]] | |||
:>>see [[Biomod/2012/Titech/Nano-Jugglers/Results/DNA_hybridization|Result]] | |||
|} | |||
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div> | |||
==DNA Design== | |||
:{| | |||
|- | |||
|width="300px"|[[Image:255247_melt.png|center|300px]] | |||
| | |||
We used NUPACK and designed five strands of DNA.<br> | |||
:>>see more [[Biomod/2012/Titech/Nano-Jugglers/Methods/DNA_Design|Method]] | |||
|} | |||
<div align = "right" style="padding-right:200px">[[#TOP|↑]]</div> |
Revision as of 19:19, 25 September 2012
<html> <head> <style type="text/css"> /* ====================
主に全体に関わるCSS
==================== */ body.mediawiki {
font-size: 14px;
background-image:url(http://openwetware.org/images/2/2d/TNJback.png); background-repeat: repeat; font-family: Calibri, Verdana, helvetica, sans-serif; }
h1 { padding: 0px 20px 5px 20px; font-size: 34px; line-height: 40px; font-weight: bold; } h2 { padding: 20px 20px 5px 20px; font-size: 25px; color: #000000; text-decoration: none; font-weight: bold; } h2 a { color: #eb8300; } h3 { padding: 20px 20px 5px 20px; font-size: 20px; color: #000; font-decoration: none; font-weight: bold; } h1.firstHeading {
display: none;
}
p { text-align: justify; } a:link { color: #00a5ea; text-decoration: none } a:visited { color:#00a5ea; text-decoration: none } a:hover {
color: #eb8300;
text-decoration: none } a:active { color:#f29400; text-decoration: none } #content{ margin: 0px 0 0px 0; align: center; padding: 12px 12px 12px 12px; width: 946px; background-color: #ffff; border: 0; } #bodyContent{ width: 970px; margin: 150px auto 0 auto; align: center; background-color: #ffffff; } #globalWrapper{ margin: 20px 0 0 0; width:994px; background-color: #ffffff; margin-left: auto; margin-right: auto } #biomodlink{ position:absolute;top:0px; width:970px;height:20px; background-color: #ffffff; margin:3px auto 3px auto }
/* ====================
メニューの画像を変更できる部分 ==================== */
#header {
margin: 0 auto 0 auto; position:absolute; top:28px; width:968px;height:189px;
background-color: #FFFFFF; background-repeat: no-repeat;
background-image: url(http://openwetware.org/images/7/74/Title-LOGO6.jpg);
} /* ====================
以下、特殊なclassに適用される ==================== */
#navigation { position:absolute;
top:120px; width:970px; height:92px; background-repeat: no-repeat;
color: #0000FF; }
/* ====================
ここからプルダウン周辺のデザイン ==================== */
- menu * {
margin: 0; padding: 0; }
- menu {
position:absolute;
font-family: calibri, verdana, sans-serif;
font-color: #000000;
background-color: transparent; float:left; display: block; }
- menu ul {
position:relative;
float: left; text-align:center; list-style: none; }
- menu li {
background-color:yellow;
position:relative;
float:left;
width: 136px;
font-size: 23px;
font-weight: bold;
border:ridge 0px #FFFFFF; }
- menu li.ach {
background-color:yellow;
position:relative;
float:left;
width: 162px;
font-size: 23px;
font-weight: bold;
border:ridge 0px #FFFFFF; }
- menu li.sup {
background-color:yellow;
position:relative;
float:left;
width: 109px;
font-size: 17px;
font-weight: bold;
border:ridge 0px #FFFFFF; }
- menu li.none {
background-color:yellow;
position:relative;
float:left;
width: 152px;
font-size: 17px;
font-weight: bold;
border:ridge 0px #FFFFFF; }
- menu a {
color: #000000; display:block; text-decoration: none; }
- menu a:hover {
background-color:gold; }
- column-one { display:none; width:0px;}
.container{background-color: #ffffff; margin-top:0px} .OWWNBcpCurrentDateFilled {display: none;}
- footer{position: center; width: 994px}
@media screen {
body { background: #F5F5F5 0 0 no-repeat; /* changed default background */ }
} </style> </head> <BODY> <div id="biomodlink"> <<a href="http://openwetware.org/wiki/Biomod">BIOMOD</a>|<a href="http://openwetware.org/wiki/Biomod/2012">2012</a>|Titech Nano-Jugglers </div> <div id="header"> <div id="navigation"> <div id="menu"> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers"><br>Home<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Team/Students"><br>Team<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Project"><br>Project<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Results">Results<br>&<br>Methods</a></font></li> <li class="ach"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Achievements"><br>Achievements<br><br></a> <li class="sup"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Protocols"><br>Suppl. Info.<br><br></a></li> <li class="none"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Acknowledgement"><br>Acknowledgements<br><br></a></li> </ul> </div> </div> </div> </BODY> </html>
Methods
DNA conjugation
Physical Vapor Deposition
Azobenzene Tweezers
Azobenzene including DNA can easily dissociate its duplex by irradiating UV-light. We put this switching system in JET body and enabled its control.
DNA hybridization in solution of H2O2
We used PAGE electrophoresis to ascertain the stability of DNA duplex in thin H₂O₂ solution 1%~5%.
DNA Design
We used NUPACK and designed five strands of DNA.
- >>see more Method