Biomod/2012/Team Sendai Old: Difference between revisions
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All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes. A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell. Here, we decided to create a novel device with such a function. We have designed a cylindrical injector/extractor device made of DNA origami. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones. We also | All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes. A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell. Here, we decided to create a novel device with such a function. We have designed a cylindrical injector/extractor device made of DNA origami. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones. We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane. | ||
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<div style="text-align:center;"><img src="http://openwetware.org/images/4/46/Format_D-Heart.jpg" alt="D-Heart" width="600"></div> | <div style="text-align:center;"><img src="http://openwetware.org/images/4/46/Format_D-Heart.jpg" alt="D-Heart" width="600"></div> | ||
Revision as of 00:27, 18 October 2012
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<!-- コンテナ --> <div id="Container">
<!-- ヘッダー --> <div id="Header"> <h1><!--Team Sendai--><img src="http://openwetware.org/images/1/1c/Headtitle2.png"></h1> <p>Tohoku University</p> </div>
<!—トップメニュー --> <ul id="Topmenu"> <li><a href=" http://openwetware.org/wiki/Biomod/2012/Tohoku/Team_Sendai">Top</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Project">Project</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Simulation">Simulation</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Results_%26_Discussion">Experiment</a></li> <li><a href=" http://openwetware.org/wiki/BIOMOD/2012/Team_Sendai/Movie">Diary</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Member ">Team</a></li> </ul>
<!-- コンテンツ --> <div id="Content">
<h2>Abstract</h2>
<p> All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes. A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell. Here, we decided to create a novel device with such a function. We have designed a cylindrical injector/extractor device made of DNA origami. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones. We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane. </p> <div style="text-align:center;"><img src="http://openwetware.org/images/4/46/Format_D-Heart.jpg" alt="D-Heart" width="600"></div>
<p> <strong>Selector</strong>: Single stranded DNA. Once the outer-most ssDNA binds to a target, the target is passed to the inner-ones one by one.</br> <strong>Gate</strong>: Cylindrical structure connecting the inside and outside like gates. A cascade of the "Selector" is planted in this. We can use this as an injector or extractor.</br> <strong>Membrane</strong>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.</br> </p>
<h2>Movie</h2> <p>Coming soon</p>
</div>
<!—フッターメニュー --> <ul id="Menu2"> <li><a href=" http://openwetware.org/wiki/Biomod/2012/Tohoku/Team_Sendai">Top</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Project">Project</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Simulation">Simulation</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Results_%26_Discussion">Experiment</a></li> <li><a href=" http://openwetware.org/wiki/BIOMOD/2012/Team_Sendai/Movie">Diary</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Member ">Team</a></li> </ul>
<!-- フッター --> <div id="Footer"> <address>Copyright (C) Team Sendai, All rights reserved.</address> </div>
<a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Top
">Legacy--Team Sendai B HP</a> </div>
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